Turunçgil Bahçelerinde Meyve Dökümüne Neden Olan Fungal Patojenlerin Tanısı ve Bazı Bileşiklerinin Antifungal Etkileri

Aysun Uysal; Şener Kurt; Soner Soylu; Merve Kara; Emine Mine Soylu

doi:10.18016/ksutarimdoga.vi.1457700

EN TR

Identification of Fungal Pathogens Causing Fruit Drop in Citrus Orchards and Antifungal Effects of Some Compounds

Abstract

This study identified the fungal agents causing fruit drop in citrus orchards in Hatay province and investigated the in vitro antifungal effects of boron compounds and pyroligneous acid (PA) against these pathogens. Thirty isolates were obtained from orange, mandarin, lemon, and grapefruit orchards in the Erzin, Dörtyol, Arsuz, and Samandağ districts of Hatay. Pathogen isolation was performed on the PDA medium. The isolates were then identified through microscopic examinations, DNA isolation, PCR, and sequencing studies. Morphological and molecular identification revealed Fusarium oxysporum, Alternaria alternata, Colletotrichum gloeosporioides, Colletotrichum karsti, and Diaporthe foeniculina as the disease agents responsible for fruit drop. Pathogenicity tests confirmed all isolates as pathogenic. Colletotrichum spp. exhibited the highest virulence with 92.6-88.9%, followed by D. foeniculina at 66.7%, F. oxysporum at 55.6%, and A. alternata at 44.4%. The antifungal effects of boron compounds and various concentrations of PA (0.03%, 0.05%, 0.07%, 0.09%, 0.1%, 0.12%, 0.15%, 0.3%, 0.5%, 0.7%, 1.0%, 1.5%; w/v) on the mycelial growth and conidial germination of five fungal pathogen isolates were investigated in vitro. Mycelial growth was completely inhibited by boric acid (0.12%), borax (0.1%), ethidote-67 (0.1%), and PA (1.5%). Additionally, boric acid (0.15%), borax (0.12%), Etidot-67 (0.12%), and PA (1.5%) significantly inhibited conidial germination. The EC50 values for mycelial growth ranged from 0.059% to 0.69%, and for conidial germination, from 0.065% to 0.82%. This is the first study investigating the antifungal activity of boron compounds and PA against fungal pathogens causing citrus fruit drop in Türkiye.

Keywords

Turunçgil Bahçelerinde Meyve Dökümüne Neden Olan Fungal Patojenlerin Tanısı ve Bazı Bileşiklerinin Antifungal Etkileri

Öz

Bu çalışmada, Hatay ilinde turunçgil bahçelerinde meyve dökümüne neden olan fungal etmenlerin belirlenmesi ve bu patojenlere karşı bor bileşikleri ve pirolignöz asitin (PA) in vitro antifungal etkileri araştırılmıştır. Hatay’ın Erzin, Dörtyol, Arsuz ve Samandağ ilçelerinde yer alan portakal, mandarin, limon, greyfurt bahçelerinde yere dökülen meyvelerden toplam 30 adet izolat elde edilmiştir. Elde edilen izolatlardan PDA besi yerinde hastalık izolasyonu yapılmıştır. Sonra bu izolatlardan mikroskop incelemeleri, DNA izolasyonu, PCR ve sekanslama çalışmaları ile teşhisleri yapılmıştır. Morfolojik ve moleküler tanılama çalışmaları sonucunda Fusarium oxysporum, Alternaria alternata, Colletotrichum gloeosporioides, C. karsti, Diaporthe foeniculina meyve dökümlerinden sorumlu hastalık etmenleri olarak teşhis edilmiştir. Yapılan patojenisite denemeleri sonucunda tüm fungal izolatlar patojen olarak belirlenmiştir. Elde edilen fungal etmenler içerisinde en yüksek virülenslik %92.6-88.9 ile Colletotrichum spp., tarafından gösterilmiş olup bu türleri %66.7 ile D. foeniculina, %55.6 ile F. oxysporum ve %44.4 ile A. alternata türleri takip etmiştir. Bor bileşikleri ve PA’nın farklı konsantrasyonları (%0.03, 0.05, 0.07, 0.09, 0.1, 0.12, 0.15, 0.3, 0.5, 0.7,1.0,1.5; w/v) elde edilen 5 fungal patojen izolatın misel gelişimi ve konidi çimlenmesi üzerindeki antifungal etkileri in vitro koşullarda araştırılmıştır. Fungal türlerin miseliyal gelişimi, farklı dozlarda borik asit (%0.12), boraks (%0.1), etidot-67 (%0.1) ve PA (%1.5) uygulamaları tarafından tamamen engellenmiştir. Öte yandan borik asit (%0.15), boraks (%0.12), Etidot-67 (%0.12) ve PA (%1.5) uygulamaları, izolatların konidi çimlenmesini önemli ölçüde engellemiştir. Elde edilen fungal türlere karşı EC50 değerleri, misel gelişimi için %0.059-0.69 ve konidi çimlenme için %0.065-0.82 arasında bulunmuştur. Bu çalışma, Türkiye’de turunçgil meyve dökümlerine neden olan fungal hastalık etmenlerine karşı bor bileşikleri ve PA’nın antifungal etkinliğini araştıran ilk çalışmadır.

Anahtar Kelimeler

Supporting Institution

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı

Project Number

21.GAP.047

Ethical Statement

Çalışmada herhangi bir anket çalışması yapılmadığı gibi, denemelerde insan ve hayvan denek kullanılmamıştır.

Thanks

Bu çalışma, Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı tarafından finansal olarak desteklenmiş (Proje Numarası: 21.GAP.047), ve Hatay Mustafa Kemal Üniversitesi Bitki Sağlığı Kliniği Uygulama ve Araştırma Merkezi laboratuvarlarında gerçekleştirilmiştir.

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Details

Primary Language

Turkish

Subjects

Phytopathology

Journal Section

Research Article

Authors

Aysun Uysal ^*
0000-0002-9067-285X
Türkiye

Şener Kurt
0000-0003-4545-5968
Türkiye

Soner Soylu
0000-0003-1002-8958
Türkiye

Merve Kara
0000-0001-7320-3376
Türkiye

Emine Mine Soylu
0000-0001-5961-0848
Türkiye

Early Pub Date

August 15, 2024

Publication Date

November 7, 2024

Submission Date

March 23, 2024

Acceptance Date

June 11, 2024

Published in Issue

Year 2024 Volume: 27 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1457700

IZ

https://izlik.org/JA34XZ23KR

Cite

RIS / Bibtex

APA

Uysal, A., Kurt, Ş., Soylu, S., Kara, M., & Soylu, E. M. (2024). Turunçgil Bahçelerinde Meyve Dökümüne Neden Olan Fungal Patojenlerin Tanısı ve Bazı Bileşiklerinin Antifungal Etkileri. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(6), 1401-1413. https://doi.org/10.18016/ksutarimdoga.vi.1457700

Turunçgil Bahçelerinde Meyve Dökümüne Neden Olan Fungal Patojenlerin Tanısı ve Bazı Bileşiklerinin Antifungal Etkileri

Identification of Fungal Pathogens Causing Fruit Drop in Citrus Orchards and Antifungal Effects of Some Compounds

Abstract

Keywords

Turunçgil Bahçelerinde Meyve Dökümüne Neden Olan Fungal Patojenlerin Tanısı ve Bazı Bileşiklerinin Antifungal Etkileri

Öz

Anahtar Kelimeler

Supporting Institution

Project Number

Ethical Statement

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Hatay İlinde Protea Süs Bitkilerinde Kurumaya Sebep Olan Fungal Hastalıklar

Determination of antifungal effects of boron products against fungal disease agents causing rots in carrots

Plant parasitic nematodes and their population densities determined in important crop plants in Hatay province, Türkiye