Endofit ve Epifit Bakteri İzolatlarının Bazı Turunçgil Fungal Hastalık Etmenlerine Karşı in vitro Biyokontrol Etkinlik ve Etki Mekanizmalarının Belirlenmesi

Yusuf Gümüş; Emine Mine Soylu

doi:10.18016/ksutarimdoga.vi.1459337

TR EN

Endofit ve Epifit Bakteri İzolatlarının Bazı Turunçgil Fungal Hastalık Etmenlerine Karşı in vitro Biyokontrol Etkinlik ve Etki Mekanizmalarının Belirlenmesi

Öz

Bu çalışmada, sağlıklı turunçgil çeşitlerinden izole edilen 48 farklı antagonist biyolojik mücadele etmeni (BCA) ve bitki gelişimini teşvik eden (PGP) endofit ve epifit bakteri izolatının turunçgillerde sorun bazı fungal hastalık etmenlerine (Colletotrichum gloeosporioides, Fusarium solani, Geotrichum citri-aurantii) karşı in vitro antagonistik etkinlikleri ve etkinlik mekanizmalarının belirlenmesi amaçlanmıştır. MALDI-TOF tanılama çalışmaları sonucunda 33 izolat Gram-pozitif (Bacillus, Lysinibacillus, Cronobacter, Staphylococcus) 15 izolat ise Gram-negatif (Acinetobacter, Pseudomonas, Kosakonia, Enterobacter, Herbaspirillum, Klebsiella, Pantoea, Rahnella, Raoultella, Rhizobium, Siccibacter) bakteri cinslerine ait türler olarak belirlenmiştir. İkili kültür testlerinde Bacillus vallismortis YGL73ep, B. thuringiensis YGT22en, B. subtilis YGS5en, B. cereus YGK25en ve Pseudomonas chlororapsis YGM82ep izolatları G. citri-aurantii, C. gloeosporioides ve F. solani’nin misel gelişimini %65.5-77.2 oranlarında engelleyen en etkili antagonist bakteri izolatları olarak belirlenmiştir. Temsili olarak seçilen P. chlororapsis YGM82ep, B. vallismortis 73YGep, B. thuringiensis 22YGen ve B. cererus 25YGen izolatları tarafından üretilen uçucu organik bileşikler (VOC’s) fungal etmenlerin in vitro misel gelişimlerini önemli ölçüde engellemiştir. Test edilen 25 bakteri izolatının, 20 tanesi siderofor, 24 tanesi amonyak, 19 tanesi protease, 5 tanesi ise hidrojen siyanür üretiminde pozitif etkinlik göstermiştir. Bakteri izolatlarından 21 tanesi IAA hormonu üretmiş, 7 izolatın ise fosforu çözebilme yeteneğinde olduğu belirlenmiştir. Elde edilen sonuçlar, yüksek düzeyde antagonist ve PGP etkinliği gösteren BCA izolatlarının, turunçgil fungal hastalıkların baskılanmasında biyopreparat, yetiştiriciliğinde ise biyogübre olarak kullanılma potansiyeline sahip olduğunu göstermiştir.

Anahtar Kelimeler

Determination of in vitro Biocontrol Efficacy and Mechanisms of Action of Endophytic and Epiphytic Bacterial Isolates against Some Citrus Fungal Disease Agents

Abstract

In this study, it was aimed to determine the in vitro antagonistic activities and mechanisms of activity of 48 different antagonist biological control agents (BCA) and plant growth promoting (PGP) endophyte and epiphyte bacterial isolates against some fungal disease agents (Colletotrichum gloeosporioides, Fusarium solani, Geotrichum citri-aurantii) in citrus fruits. As a result of MALDI-TOF identification studies, 33 isolates were identified as Gram-positive (Bacillus, Lysinibacillus, Cronobacter, Staphylococcus) and 15 isolates were identified as Gram-negative (Acinetobacter, Pseudomonas, Kosakonia, Enterobacter, Herbaspirillum, Klebsiella, Pantoea, Rahnella, Raoultella, Rhizobium, Siccibacter). Bacillus vallismortis YGL73ep, B. thuringiensis YGT22en, B. subtilis YGS5en, B. cereus YGK25en and Pseudomonas chlororapsis YGM82ep isolates were found to be the most effective antagonist isolates suppressing mycelial growth of G. citri-aurantii, C. gloeosporioides and F. solani by 65.5-77.2% in in vitro dual culture tests. Volatile organic compounds (VOCs) produced by representative isolates P. chlororapsis YGM82ep, B. vallismortis 73YGep, B. thuringiensis 22YGen, and B. cererus 25YGen significantly inhibited in vitro mycelial growth of fungal agents. Of the 25 bacterial isolates tested, 20 isolates were positive for siderophore, 24 isolates for ammonia, 19 isolates for protease, and 5 isolates for hydrogen cyanide production. Of the bacterial isolates tested, 21 isolates produced the hormone IAA and 7 isolates were found to be capable of solubilizing phosphorus. The results showed that the BCA isolates with high levels of antagonistic and PGP activities have the potential to be used as biopreparat for the suppression of citrus fungal diseases and also as biofertilizer in citrus cultivation.

Keywords

Supporting Institution

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu

Project Number

Proje Numarası: 20.YL.022

Ethical Statement

Yazarlar makaleye eşit oranda katkı sağlamış olduklarını beyan eder. Makale yazarları aralarında herhangi bir çıkar çatışması olmadığını beyan ederler. Çalışma için Etik Kurul Onayına ihtiyaç bulunmamaktadır.

Thanks

Bu çalışma Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu tarafından finansal olarak desteklenmiştir (Proje Numarası: 20.YL.022).

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Details

Primary Language

Turkish

Subjects

Phytopathology

Journal Section

Research Article

Authors

Yusuf Gümüş
0000-0001-7818-1831
Türkiye

Emine Mine Soylu ^*
0000-0001-5961-0848
Türkiye

Early Pub Date

August 15, 2024

Publication Date

November 7, 2024

Submission Date

March 26, 2024

Acceptance Date

May 8, 2024

Published in Issue

Year 2024 Volume: 27 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1459337

IZ

https://izlik.org/JA78EF26ET

Cite

RIS / Bibtex

APA

Gümüş, Y., & Soylu, E. M. (2024). Endofit ve Epifit Bakteri İzolatlarının Bazı Turunçgil Fungal Hastalık Etmenlerine Karşı in vitro Biyokontrol Etkinlik ve Etki Mekanizmalarının Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(6), 1376-1391. https://doi.org/10.18016/ksutarimdoga.vi.1459337