Determination of Genome Size Differentiation and Ploidy Levels in Some Citrus Rootstock Populations

Sefa Polatöz; Murat Şeker; Çağlar Kaya

doi:10.18016/ksutarimdoga.vi.1475151

TR EN

Bazı Turunçgil Anaç Popülasyonlarında Genom Büyüklüğü Farklılıklarının ve Ploidi Seviyelerinin Belirlenmesi

Öz

Bitki ıslahında kullanılan materyalin ploidi seviyesinin belirlenmesi, özellikle biyoteknoloji uygulamaları açısından önemlidir. Turunçgillerdeki genetik çeşitlilik, farklı iklim ve topraklara uyumlu anaç ve çeşitlerin geliştirilmesini sağlamaktadır. Ticari üretimde çeşitli uygun anaçlar kullanılmaktadır. Bu çalışma, dünya genelinde yaygın olarak kullanılan turunçgil anaçlarının flow sitometri ile genom büyüklüklerini ve ploidi seviyelerinin belirlenmesi amacıyla yürütülmüştür. Çalışmada Gou-Tou, C-35, Troyer Citrange, Taiwanica, Citremon, Yuzu, Sunki mandarin, Flying Dragon, Yuma Citrange, Macrophylla ve Çin portakalı anaçları kullanılmıştır. İlgili anaçlardan elde edilen taze yaprak dokuları standart tür olarak kullanılan triploid Tahiti limon yaprak dokusu ile karıştırılmış ve hücre çekirdekleri izole edilmiştir. Propidium iodid ile boyanmış hücreler flow sitometri ile okunmuş ve histogram ve sitogramlar elde edilmiştir. Elde edilen sonuçlara göre, tüm türlerin diploid genom büyüklüğüne sahip olmalarına karşın genom hacmi açısından türler arasında farklılıkların olduğu belirlenmiştir. Yuzu fidanlarının en büyük (0.808 pg/2C), Flying Dragon trifoliatın ise en küçük genom hacmine sahip olduğu (0.700 pg/2C) tespit edilmiştir.

Anahtar Kelimeler

Determination of Genome Size Differentiation and Ploidy Levels in Some Citrus Rootstock Populations

Abstract

Determining the ploidy level of plant material used in breeding is important, especially for biotechnological applications. The genetic diversity in citrus enables the development of rootstocks and cultivars adapted to various climates and soils. Various suitable rootstocks are used in commercial citrus production.This study was conducted to determine the genome size and ploidy levels of citrus rootstocks widely used worldwide using flow cytometry. The rootstocks used in the study included Gou-Tou, C-35, Troyer citrus, Taiwanica, Citremon, Yuzu, Sunki mandarin, Flying Dragon, Yuma citrus, Macrophylla and Chinese orange. Fresh leaf tissue from each rootstock was mixed with triploid Tahitian lemon leaf tissue, used as a standard species, and cell nuclei were isolated. The cells stained with propidium iodide were analysed by flow cytometry, and histograms and cytograms were obtained. According to the results, although all species had diploid genome sizes, differences were observed between species in terms of genome volume. Yuzu seedlings were found to have the largest genome size (0.808 pg/2C), while Flying Dragon trifoliate had the smallest genome size (0.700 pg/2C).

Keywords

References

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Details

Primary Language

English

Subjects

Plant Biotechnology , Pomology and Treatment

Journal Section

Research Article

Authors

Sefa Polatöz ^*
0000-0001-8219-3325
Türkiye

Murat Şeker
0000-0002-6886-0547
Türkiye

Çağlar Kaya
0000-0002-7054-3081
Türkiye

Early Pub Date

May 3, 2025

Publication Date

June 9, 2025

Submission Date

April 29, 2024

Acceptance Date

May 3, 2025

Published in Issue

Year 2025 Volume: 28 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1475151

IZ

https://izlik.org/JA24NX22UH

Cite

RIS / Bibtex

APA

Polatöz, S., Şeker, M., & Kaya, Ç. (2025). Determination of Genome Size Differentiation and Ploidy Levels in Some Citrus Rootstock Populations. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(3), 736-745. https://doi.org/10.18016/ksutarimdoga.vi.1475151