Centaurea lycaonica Boiss. & Heldr. Bitkisinin İnsan Servikal Kanser Hücre Hattında Sitotoksisitesinin MTT Testi ve xCELLigence Sistemi ile Değerlendirilmesi

Ayşe Kübra Karaboğa Arslan; Eylül Güngörenler; Leyla Paşayeva; Mehmet Bozkurt; Osman Tugay

doi:10.18016/ksutarimdoga.vi.1496499

EN TR

Evaluation of Cytotoxicity of Centaurea lycaonica Boiss. & Heldr. Plant on Human Cervical Cancer Cell Line with MTT Test and xCELLigence System

Abstract

Many species belonging to the genus Centaurea L. are endemic in various parts of the world and are used in folk medicine. The cytotoxicity of some species belonging to this genus on cervical cancer cell lines was investigated. In this study, it was aimed to investigate the cytotoxic effect of dichloromethane (CRD) and methanol (CRM) extracts prepared by 24 h maceration method on human cervical cancer cell line (HeLa) at 48 h exposure based on the root part of Centaurea lycaonica, which is endemic to Turkey and about which there are almost no studies. The effect of different concentrations of plant extracts on cell viability was investigated using MTT and xCELLigence RTCA systems, which have different working principles, and IC50 values were determined. As a result, MTT findings of CRD and CRM extracts were consistent with xCELLigence analysis and found to have a cytotoxic effect on HeLa cells. It was thought that C. lycaonica species could be evaluated as a new natural source in cancer treatment. It is thought that this study will encourage the elucidation of the biological activities and mechanisms of action of many species endemic to Turkey.

Keywords

Centaurea lycaonica Boiss. & Heldr. Bitkisinin İnsan Servikal Kanser Hücre Hattında Sitotoksisitesinin MTT Testi ve xCELLigence Sistemi ile Değerlendirilmesi

Öz

Centaurea L. cinsine ait birçok tür, dünyanın çeşitli bölgelerinde endemik olarak varlığını sürdürmekte ve halk hekimliğinde kullanımlarıyla literatürde yerini almaktadır. Söz konusu cinse ait bazı türlerin servikal kanser hücre hattı üzerindeki sitotoksisitesi incelenmiştir. Bu çalışmada Türkiye’ye endemik ve hakkında yok denecek kadar az sayıda çalışma bulunan Centaurea lycaonica türünün kök kısmından hareketle, 24 saat maserasyon yöntemiyle hazırlanmış diklorometan (CRD) ve metanol (CRM) ekstrelerinin 48 saatlik maruziyette insan servikal kanser hücre hattındaki (HeLa) sitotoksik etkisinin araştırılması amaçlandı. Bitki ekstresinden hazırlanan farklı konsantrasyonların hücre canlılığına etkisi çalışma prensipleri farklı olan MTT ve xCELLigence GZHA sistemi kullanılarak araştırıldı ve IC50 değerleri belirlendi. Sonuç olarak, CRD ve CRM ekstrelerinin MTT bulguları, xCELLigence analiziyle tutarlı olup HeLa hücrelerinde sitotoksik etkiye sahip olduğu bulundu. C. lycaonica türünün kanser tedavisinde yeni bir doğal kaynak olarak değerlendirilebileceği düşünüldü. Bu çalışmanın, Türkiye’ye endemik birçok türün biyolojik aktivite ve etki mekanizmalarının aydınlatılmasını teşvik edeceği düşünülmektedir.

Anahtar Kelimeler

Supporting Institution

TÜBİTAK

Project Number

1919B012314005

Thanks

Hazırlanmış olan bu araştırma çalışması, TÜBİTAK 2209-A kapsamında 1919B012314005 kodu ile desteklenen bitirme projesinden üretilmiş olup katkılarından dolayı TÜBİTAK’a teşekkürlerimizi sunarız.

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Details

Primary Language

Turkish

Subjects

Structural Biology

Journal Section

Research Article

Authors

Ayşe Kübra Karaboğa Arslan ^*
0000-0002-4689-0657
Türkiye

Eylül Güngörenler
0009-0008-4009-268X
Türkiye

Leyla Paşayeva
0000-0003-3860-7222
Türkiye

Mehmet Bozkurt
0000-0003-2965-6719
Türkiye

Osman Tugay
0000-0003-3980-7648
Türkiye

Early Pub Date

January 30, 2025

Publication Date

February 12, 2025

Submission Date

June 5, 2024

Acceptance Date

December 31, 2024

Published in Issue

Year 2025 Volume: 28 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1496499

IZ

https://izlik.org/JA24ZN83ZF

Cite

RIS / Bibtex

APA

Karaboğa Arslan, A. K., Güngörenler, E., Paşayeva, L., Bozkurt, M., & Tugay, O. (2025). Centaurea lycaonica Boiss. & Heldr. Bitkisinin İnsan Servikal Kanser Hücre Hattında Sitotoksisitesinin MTT Testi ve xCELLigence Sistemi ile Değerlendirilmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(1), 47-52. https://doi.org/10.18016/ksutarimdoga.vi.1496499

Cited By

Ordu İli Yöresel Bitkilerinin (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum) İnsan Serviks Kanseri (HeLa) Hücre Hattına Karşı Antikanser Etkilerinin ve DNA Koruyucu Aktivitelerinin Araştırılması

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1651205

Phytochemical Profiling and Molecular Insights of Centaurea lycaonica: Apoptosis Induction via the Intrinsic Pathway in Endometrial Cancer Cells

Pharmaceuticals

https://doi.org/10.3390/ph18101558