Sex Determination in Young and Adult Rainbow Trout Using Geometric Morphometrics Analysis

Nimet Turgut; Saadettin Tıpırdamaz; Hakan Yalçın

doi:10.18016/ksutarimdoga.vi.1518236

TR EN

Geometrik Morfometri Analiz Yöntemi Kullanılarak Genç ve Erişkin Gökkuşağı Alabalıklarında Cinsiyet Tayini

Öz

Gökkuşağı alabalıklarında cinsiyet ayrımındaki genel yaklaşım, eşeysel olgunluğa erişmiş bireylerde vücut şekli ve renginin öznel değerlendirmesidir. Bu çalışmanın amacı, genç ve erişkin gökkuşağı alabalıkları arasındaki vücut geometrisine dayalı cinsiyet farklılıklarını geometrik morfometri ile değerlendirmektir. Çalışma 40 genç (20 dişi, 20 erkek) ve 40 erişkin (20 dişi, 20 erkek) olmak üzere toplam 80 adet gökkuşağı alabalığı (Oncorhynchus mykiss) üzerinde gerçekleştirildi. Tüm alabalıkların sol lateral yönlü görüntüleri üzerinde 16 adet homolog landmark kullanılmıştır. Cinsiyetler arasında ayrımın yapılabilmesi için genç ve erişkin balıklara ayrı ayrı geometrik morfometrik prosedürler uygulanmıştır. Yapılan analizler sonucunda, genç erkek alabalık örneklerinde dişilere göre, pelvik yüzgecin orijin noktasının posterioventral yönlü olduğu ve burnun uç noktasının ise anteriodorsal yönelim gösterdiği belirlendi. Erişkin erkek alabalıkların dişilerle karşılaştırıldığında, burnun uç noktasının anteriodorsal yönlü olduğu, dorsal yüzgeçin anteriodorsal köşe noktasının ise dorsal yönlü olduğu tespit edildi. PCA (Principal Component) skorları, PCA 1-2’nin cinsiyet grupları arasındaki toplam varyansın gençlerde %61.49’unu erişkinlerde %43.48’ini açıkladığını göstermektedir. Geometrik morfometri ile özellikle genç, kısmen de erişkin gökkuşağı alabalıklarında eşeysel farklılıklar kolaylıkla tespit edildi. Sonuç olarak ucuz, invaziv olmayan ve herkesin erişebileceği pratik bir yöntem olan geometrik morfometrinin cinsiyetin tanımlamasına yardımcı olarak, alabalık yetiştiriciliği yapan işletmelerin kısa sürede kolay, sürdürülebilir, ekonomik ve katma değeri yüksek balık yetiştiriciliği yapabilmelerine katkı sağlayabileceğini düşünüyoruz.

Anahtar Kelimeler

Sex Determination in Young and Adult Rainbow Trout Using Geometric Morphometrics Analysis

Abstract

Sex determination in rainbow trout generally involves the subjective evaluation of body shape and color in sexually mature individuals. The present study aimed to evaluate sex differences in body geometry in both young and adult individuals of rainbow trout using geometric morphometry. A total of 80 rainbow trout (Oncorhynchus mykiss) individuals were studied, including 40 young individuals (20 females and 20 males) and 40 adult individuals (20 females and 20 males). Sixteen homologous landmarks were evaluated in the left lateral images of the studied trout. Geometric morphometrics procedures were applied separately to young and adult fish to determine the sex differences. The results revealed that in young male trout individuals, the origin point of the pelvic fin was posteroventral, and the anterior tip of the nose was anterodorsal, compared to females. When adult male trout fish were compared to their female counterparts, the anterior tip of the nose was observed to be anterodorsal, and the anterodorsal corner of the dorsal fin was dorsal. In the Principal Component Analysis (PCA), PCA 1–2 explained 61.49% of the total variance between the sexes in young trout and 43.48% of the total variation in adult trout. The use of geometric morphometry enabled easy determination of sex differences, particularly in young rainbow trout and partly in adult rainbow trout. Therefore, geometric morphometry could serve as a cost-effective, non-invasive, and feasible approach for the sex determination of fish in trout farms to achieve simple, sustainable, economical, and high-value-added fish farming within a short duration.

Keywords

Supporting Institution

Selcuk University, the Academic Staff Training Program Coordination Unıt, (Project Number: 2014-OYP-022) provided the fund support

Project Number

2014-OYP-022

Ethical Statement

All procedures were carried out after receiving Experimental Animal Production and the Research Center at the Faculty of Veterinary Medicine, Selcuk University Ethics Committee approval (2015/90).

Thanks

This study was derived from a part of the M.S. thesis prepared by the first author under the supervision of the other authors. This study was supported by the Selcuk University, the Academic Staff Training Program Coordination Unıt, (Project Number: 2014-OYP-022). Findings of a part of the investigation, the abstract was presented as oral presentation presentation at the 1st International Veterinary Anatomy Congress and was published as a summary text in the congress book. The authors would like to thank Assistant Professor Dr. Sedat Aydoğdu and Murat Dil for their help during sample receiving.

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Details

Primary Language

English

Subjects

Fish Anatomy

Journal Section

Research Article

Authors

Nimet Turgut ^*
0000-0001-6446-2958
Türkiye

Saadettin Tıpırdamaz
0000-0003-4786-2612
Türkiye

Hakan Yalçın
0000-0001-6102-9665
Türkiye

Early Pub Date

December 19, 2024

Publication Date

December 31, 2024

Submission Date

July 18, 2024

Acceptance Date

October 24, 2024

Published in Issue

Year 2024 Volume: 27 Number: Ek Sayı 2 (Suppl 2)

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1518236

IZ

https://izlik.org/JA23DA58KB

Cite

RIS / Bibtex

APA

Turgut, N., Tıpırdamaz, S., & Yalçın, H. (2024). Sex Determination in Young and Adult Rainbow Trout Using Geometric Morphometrics Analysis. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(Ek Sayı 2 (Suppl 2), 533-541. https://doi.org/10.18016/ksutarimdoga.vi.1518236