Preliminary Insights into the Phylogeny of Colchicum Species

Ezgi Çabuk Şahin

doi:10.18016/ksutarimdoga.vi.1518459

TR EN

Colchicum Türlerinin Filogenisine İlişkin Ön Bilgiler

Öz

Colchicum, sahip olduğu doğal özellikleri nedeniyle tıp alanında önemli bir rol oynamış olup, önemi geçmişten günümüze kadar uzanmaktadır. DNA barkodlama, bitki türlerinin tanımlanması ve korunması için etkili bir araçtır. Bu çalışma, Colchicum türlerinin filogenetik yapısının oluşturulmasında 18S rRNA geninin uygulamasını test etmiştir. 33 Colchicum örneği ve bir dış grup türü kullanılarak, 18S rRNA kısmi geni ile filogenetik UPGMA (aritmetik ortalama ile ağırlıksız çift grup yöntemi) ağacı ve STRUCTURE analizi yapılmıştır. Çalışmanın sonuçları, 18S rRNA kullanılarak yapılan DNA barkodlamanın Colchicum türlerinin tanımlanmasında etkili bir yöntem olduğunu ve test edilen dizilerin %97.76'sının başarılı bir şekilde belirlendiğini göstermiştir. Bu çalışma, 18S rRNA geninin filogenetik yapı oluşturmadaki faydasını göstererek Colchicum türlerinin filogenetik analizine katkıda bulunmakta ve bitki genetiğini anlama konusunda ilerlememize olanak sağlamaktadır. Tür tanımlaması için elde edilen nükleotid dizileri, uluslararası erişilebilir genetik verilere katkıda bulunmak amacıyla yaşam barkodu veri sistemine (BOLD) yüklenmiştir.

Anahtar Kelimeler

Preliminary Insights into the Phylogeny of Colchicum Species

Abstract

Colchicum has played a vital role in the field of medicine due to its inherent properties, with its importance extending from the past to the present. DNA barcoding is an effective tool for the identification and conservation of plant species. This study tested the application of the 18S rRNA gene for the phylogenetic construction of Colchicum species. The phylogenetic UPGMA (unweighted pair group method with arithmetic mean) tree and STRUCTURE analysis were conducted to assess the consistency of the results for 33 Colchicum specimens and one outgroup species, utilizing the 18S rRNA partial gene. The results of the study showed that DNA barcoding using 18S rRNA was an effective method for identifying Colchicum species, with 97.76% of the tested sequences being successfully determined. This study contributes to the field of phylogenetic analysis of Colchicum species by illustrating the utility of the 18S rRNA gene for phylogenetic construction, underscoring its significance in advancing our understanding of plant genetics. The nucleotide sequences obtained for species identification in this study were uploaded to the barcode of life data system (BOLD), contributing to internationally accessible genetic data.

Keywords

Thanks

I would like to express my gratitude to Prof. Ahu ALTINKUT UNCUOĞLU from the Department of Bioengineering at Marmara University and Prof. Yıldız AYDIN from the Department of Biology at Marmara University for their significant contributions to the completion of this study.

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Details

Primary Language

English

Subjects

Plant Biotechnology

Journal Section

Research Article

Authors

Ezgi Çabuk Şahin ^*
0000-0003-1505-5752
Türkiye

Early Pub Date

December 19, 2024

Publication Date

December 31, 2024

Submission Date

July 18, 2024

Acceptance Date

October 12, 2024

Published in Issue

Year 2024 Volume: 27 Number: Ek Sayı 2 (Suppl 2)

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1518459

IZ

https://izlik.org/JA27EC76KG

Cite

RIS / Bibtex

APA

Çabuk Şahin, E. (2024). Preliminary Insights into the Phylogeny of Colchicum Species. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(Ek Sayı 2 (Suppl 2), 350-360. https://doi.org/10.18016/ksutarimdoga.vi.1518459