Variability of Genetically Improved Farmed Tilapia (Gift Tilapia) Crossed With Upm Red Tilapia Hybrids By Mitochondrial DNA Conserved Region Sequence

Oster F. Nwachı; Awawu Dasuki

doi:10.18016/ksutarimdoga.vi.1538782

EN TR

Variability of Genetically Improved Farmed Tilapia (Gift Tilapia) Crossed With Upm Red Tilapia Hybrids By Mitochondrial DNA Conserved Region Sequence

Abstract

Mitochondrial DNA conserved region (mtDNA CR) segment of a cross between GIFT tilapia and UPM red tilapia, their F1-hybrid (F1) and Backcross (BcF1) was used to define their genetic variability. DNA was extracted from 25 randomly selected specimens of each of the test strains and mtDNA CR primer ORMT-F ORMT-R was used. The MEGA software using Maximum Parsimony (MP) was conducted for the evolutionary analysis of the strains using Tilapia rendalli (AF484717.1 accession numbers from NCBI database). A total of 441 polymorphic regions of 99 sequencing sites were observed, the sites with 12 alignment gaps were missing while the invariable monomorphic sites were 360 (81.6%) with 69 (15.6%) variable polymorphic sites. Parsimony informative sites were 68 with 1 singleton variable site. The conserved regions were effective in discriminating between the sampled fish because they contained important elements that aid replication and expression of the mitochondria genome. The clustering of the strain in the sub-tree of the phylogeny verifies a monophyletic relationship although some of the strains showed more relatedness by their consistent overlap and clustering. Similarly with 0.1 genetic variability conserve region could be used for discrimination between the strains.

Keywords

Variability of Genetically Improved Farmed Tilapia (Gift Tilapia) Crossed With Upm Red Tilapia Hybrids By Mitochondrial DNA Conserved Region Sequence

Öz

Mitochondrial DNA conserved region (mtDNA CR) segment of a cross between GIFT tilapia and UPM red tilapia, their F1-hybrid (F1) and Backcross (BcF1) was used to define their genetic variability. DNA was extracted from 25 randomly selected specimens of each of the test strains and mtDNA CR primer ORMT-F ORMT-R was used. The MEGA software using Maximum Parsimony (MP) was conducted for the evolutionary analysis of the strains using Tilapia rendalli (AF484717.1 accession numbers from NCBI database). A total of 441 polymorphic regions of 99 sequencing sites were observed, the sites with 12 alignment gaps were missing while the invariable monomorphic sites were 360 (81.6%) with 69 (15.6%) variable polymorphic sites. Parsimony informative sites were 68 with 1 singleton variable site. The conserved regions were effective in discriminating between the sampled fish because they contained important elements that aid replication and expression of the mitochondria genome. The clustering of the strain in the sub-tree of the phylogeny verifies a monophyletic relationship although some of the strains showed more relatedness by their consistent overlap and clustering. Similarly with 0.1 genetic variability conserve region could be used for discrimination between the strains.

Anahtar Kelimeler

Ethical Statement

No potential conflict of interest relevant to this article was reported.

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Details

Primary Language

Turkish

Subjects

Fish Physiology and Genetics

Journal Section

Research Article

Authors

Oster F. Nwachı
Malaysia

Awawu Dasuki ^*
0000-0001-7056-4184
Nigeria

Early Pub Date

March 20, 2025

Publication Date

March 27, 2025

Submission Date

August 27, 2024

Acceptance Date

February 13, 2025

Published in Issue

Year 2025 Volume: 28 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1538782

IZ

https://izlik.org/JA75AD23MC

Cite

RIS / Bibtex

APA

Nwachı, O. F., & Dasuki, A. (2025). Variability of Genetically Improved Farmed Tilapia (Gift Tilapia) Crossed With Upm Red Tilapia Hybrids By Mitochondrial DNA Conserved Region Sequence. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(2), 571-579. https://doi.org/10.18016/ksutarimdoga.vi.1538782

Cited By

Reproductive and Phenotypic Performance of Hybrid Crosses Between GIFT and Red Tilapia Strains

UMYU Scientifica

https://doi.org/10.56919/usci.2543.045