Determination of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonidea) Species Using DNA Barcoding in Alfalfa Fields Around Van, Türkiye

Mehmet Salih Özgökçe; Hilmi Kara; Mehmet Ramazan Rişvanlı; Furkan Harun Baş

doi:10.18016/ksutarimdoga.vi.1550912

EN TR

Determination of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonidea) Species Using DNA Barcoding in Alfalfa Fields Around Van, Türkiye

Abstract

Nowadays, DNA barcoding has become a powerful tool for rapid and accurate species identification, especially due to the limited number of expert taxonomists, difficulties in distinguishing very similar species, and difficulties in identifying preadult stages of species. In this study, species identification of Ichneumonidea species found in alfalfa fields in Van Province (Türkiye) and its surroundings was determined using DNA barcoding technique. Most of the Ichneumonidea are known as parasitoids of other arthropods and generally on holometabolous insects, primarily targeting insect larvae. Since they are used as natural enemies of harmful insects and have an important role in biological control, detailed studies have been conducted on this group and continue. Due to the limited use of pesticides, alfalfa fields in Van Province and its surroundings, where insect diversity is preserved, were selected as the study area to investigate Ichneumonidea species. The study was conducted in eight different districts of Van province, namely the Merkez, Ipekyolu, and Tuşba districts, Erciş, Muradiye, Özalp, Gürpınar, Edremit, and Başkale districts, where alfalfa is produced the most. Samples were collected between May and August, DNA sequences were extracted from the collected samples, and the sequences were compared with the species in NCBI, and high sequence similarities were observed. As a result of the study, two Braconidae and seven Ichneumonidae species were identified. Molecular identifications of Aleiodes similis (Curtis, 1834) (99.61%), Bathyplectes curculionis (Thomson, 1887) (98.14%), Enizemum ornatum (Gravenhorst, 1829) (99.80%), and Syrphoctonus elegans (Gravenhorst, 1829) (99.24%) were confirmed with high similarity rates and became the first record for Van province.

Keywords

Van İli (Türkiye) ve Çevresindeki Yonca Alanlarında DNA Barkodlama Yöntemi Kullanılarak Ichneumonidae ve Braconidae (Hymenoptera: Ichneumonidea) Türlerinin Belirlenmesi

Abstract

Günümüzde, DNA barkodlama, özellikle uzman taksonomistlerin az sayıda olması, birbirine çok benzeyen türlerin ayırt edilmesindeki zorluklar ve türlerin ergin öncesi evrelerinin tanımlanmasındaki güçlükler nedeniyle hızlı ve doğru tür tanımlaması için güçlü bir araç haline gelmiştir. Bu çalışmada, Van ili (Türkiye) ve çevresindeki yonca tarlalarında bulunan Ichneumonidea türlerinin teşhisi DNA barkodlama tekniği kullanılarak belirlenmeye çalışılmıştır. Ichneumonidea'nın çoğu diğer eklembacaklıların parazitoidleri olarak bilinir ve genellikle holometebol böcekler üzerinde bulunur. Zararlı böceklerin doğal düşmaları olarak kullanıldıklarından ve biyolojik mücadeledeki önemli rolleri nedeniyle bu grup üzerinde detaylı çalışmalar yapılmıştır ve yapılmaya devam etmektedir. Pestisit kullanımının sınırlı olması sebebiyle böcek çeşitliliğinin korunduğu Van ili ve çevresindeki yonca tarlaları, Ichneumonidea türlerini araştırmak için çalışma alanı olarak seçilmiştir. Çalışma, Van ilinin Merkez, İpekyolu ve Tuşba ilçeleri ile yoncanın en çok üretildiği Erciş, Muradiye, Özalp, Gürpınar, Edremit ve Başkale ilçeleri olmak üzere sekiz farklı ilçesinde yürütülmüştür. Örnekler Mayıs ve Ağustos ayları arasında toplanmış, toplanan örneklerden DNA dizileri çıkarılmış ve diziler NCBI'daki türlerle karşılaştırılmış ve yüksek benzerlikler elde edilmiştir. Çalışma sonucunda iki Braconidae türü ve yedi Ichneumonidae türü tespit edilmiştir. Aleiodes similis (Curtis, 1834) (%99.61), Bathyplectes curculionis (Thomson, 1887) (%98.14), Enizemum ornatum (Gravenhorst, 1829) (%99.80) ve Syrphoctonus elegans (Gravenhorst, 1829) (%99.24) moleküler teşhisleri yüksek benzerlik oranları doğrulanmış ve Van ili için ilk kayıt olmuştur.

Keywords

Supporting Institution

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projleri Koordinasyon Birimi

Project Number

FBA-2021-9542

Thanks

This work was supported by BAPB, Van Yuzuncu Yil University, Project No. FBA-2021-9542 (Turkey).

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Details

Primary Language

English

Subjects

Entomology in Agriculture

Journal Section

Research Article

Authors

Mehmet Salih Özgökçe ^*
0000-0002-6777-9149
Türkiye

Hilmi Kara
0000-0003-0580-0464
Türkiye

Mehmet Ramazan Rişvanlı
0000-0002-5882-0533
Türkiye

Furkan Harun Baş
0000-0002-4764-9742
Türkiye

Early Pub Date

February 8, 2026

Publication Date

February 8, 2026

Submission Date

September 17, 2024

Acceptance Date

September 1, 2025

Published in Issue

Year 2026 Volume: 29 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1550912

IZ

https://izlik.org/JA92SB68NP

Cite

RIS / Bibtex

APA

Özgökçe, M. S., Kara, H., Rişvanlı, M. R., & Baş, F. H. (2026). Determination of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonidea) Species Using DNA Barcoding in Alfalfa Fields Around Van, Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 29(3), 697-714. https://doi.org/10.18016/ksutarimdoga.vi.1550912

AMA

1.Özgökçe MS, Kara H, Rişvanlı MR, Baş FH. Determination of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonidea) Species Using DNA Barcoding in Alfalfa Fields Around Van, Türkiye. KSU J. Agric Nat. 2026;29(3):697-714. doi:10.18016/ksutarimdoga.vi.1550912

Chicago

Özgökçe, Mehmet Salih, Hilmi Kara, Mehmet Ramazan Rişvanlı, and Furkan Harun Baş. 2026. “Determination of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonidea) Species Using DNA Barcoding in Alfalfa Fields Around Van, Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 29 (3): 697-714. https://doi.org/10.18016/ksutarimdoga.vi.1550912.

EndNote

Özgökçe MS, Kara H, Rişvanlı MR, Baş FH (April 1, 2026) Determination of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonidea) Species Using DNA Barcoding in Alfalfa Fields Around Van, Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 29 3 697–714.

IEEE

[1]M. S. Özgökçe, H. Kara, M. R. Rişvanlı, and F. H. Baş, “Determination of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonidea) Species Using DNA Barcoding in Alfalfa Fields Around Van, Türkiye”, KSU J. Agric Nat., vol. 29, no. 3, pp. 697–714, Apr. 2026, doi: 10.18016/ksutarimdoga.vi.1550912.

ISNAD

Özgökçe, Mehmet Salih - Kara, Hilmi - Rişvanlı, Mehmet Ramazan - Baş, Furkan Harun. “Determination of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonidea) Species Using DNA Barcoding in Alfalfa Fields Around Van, Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 29/3 (April 1, 2026): 697-714. https://doi.org/10.18016/ksutarimdoga.vi.1550912.

JAMA

1.Özgökçe MS, Kara H, Rişvanlı MR, Baş FH. Determination of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonidea) Species Using DNA Barcoding in Alfalfa Fields Around Van, Türkiye. KSU J. Agric Nat. 2026;29:697–714.

MLA

Özgökçe, Mehmet Salih, et al. “Determination of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonidea) Species Using DNA Barcoding in Alfalfa Fields Around Van, Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 29, no. 3, Apr. 2026, pp. 697-14, doi:10.18016/ksutarimdoga.vi.1550912.

Vancouver

1.Mehmet Salih Özgökçe, Hilmi Kara, Mehmet Ramazan Rişvanlı, Furkan Harun Baş. Determination of Ichneumonidae and Braconidae (Hymenoptera: Ichneumonidea) Species Using DNA Barcoding in Alfalfa Fields Around Van, Türkiye. KSU J. Agric Nat. 2026 Apr. 1;29(3):697-714. doi:10.18016/ksutarimdoga.vi.1550912