Combination of Silver Nanoparticles Synthesized Using Karaerik Extract and Cisplatin: Effects on Breast Cancer Cells

Tuğba Atici; Deniz Altun Çolak

doi:10.18016/ksutarimdoga.vi.1554474

EN TR

Combination of Silver Nanoparticles Synthesized Using Karaerik Extract and Cisplatin: Effects on Breast Cancer Cells

Abstract

Nanoparticle-based therapies provide targeted drug delivery with reduced side effects, making them promising options for cancer treatment. This study examines the anticancer and oxidative effects of green-synthesized silver nanoparticles (AgNPs) derived from the Karaerik grape (Vitis vinifera L., Vitaceae), a variety known for its high antioxidant capacity, both alone and in combination with cisplatin (CP) on MDA-MB-231 breast cancer cells. AgNPs were synthesized using the plant leaf extract and characterized through UV-Vis, SEM-EDX, and FT-IR techniques. MDA-MB-231 cells were exposed to 10 and 20 μg mL⁻¹ AgNPs, 25 μM CP, and their combinations for 24, 48, and 72 hours. Cytotoxicity was analyzed using the MTT assay, and IC50 values were determined. Parameters of oxidative stress, including total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI), were evaluated to assess oxidative balance disruption. The combination of AgNPs and CP led to a significantly greater reduction in cell viability compared to individual treatments (p<.05). The IC50 value of CP decreased in the presence of AgNPs, indicating an enhanced cytotoxic effect. Additionally, a significant increase in TOS and OSI levels, along with a decrease in TAS, suggested that the combination therapy induced oxidative stress-mediated cell death. Green-synthesized AgNPs from Karaerik grape leaves enhance CP-induced cytotoxicity by modulating oxidative stress pathways, indicating their potential role as adjuvants in breast cancer therapy. These findings underscore the importance of green nanotechnology-based strategies in oncology.

Keywords

Karaerik Ekstraktı ile Sentezlenen Gümüş Nanopartiküllerin Sisplatin ile Kombinasyonu: Meme Kanseri Hücrelerine Etkileri

Öz

Nanopartikül bazlı tedaviler, azaltılmış yan etkilerle hedefe yönelik ilaç dağıtımı sağlayarak kanser tedavisinde umut vaat etmektedir. Bu çalışmada, yüksek antioksidan kapasiteye sahip bir üzüm çeşidi olan Karaerik üzümünden (Vitis vinifera L., Vitaceae) elde edilen yeşil sentezlenmiş gümüş nanopartiküllerin (AgNPs) tek başına ve sisplatin (CP) ile kombinasyon halinde MDA-MB-231 meme kanseri hücreleri üzerindeki antikanser ve oksidatif etkileri araştırılmaktadır. AgNP’ler bitki yaprağı ekstraktı kullanılarak sentezlenmiş ve UV-Vis, SEM-EDX ve FT-IR teknikleri ile karakterize edilmiştir. MDA-MB-231 hücreleri 10 ve 20 μg mL-¹ AgNP’lere, 25 μM CP’ye ve bunların kombinasyonlarına 24, 48 ve 72 saat boyunca maruz bırakılmıştır. Sitotoksisite MTT deneyi kullanılarak değerlendirilmiş ve IC₅₀ değerleri belirlenmiştir. Oksidatif denge bozulmasını belirlemek için toplam oksidan durum (TOS), toplam antioksidan durum (TAS) ve oksidatif stres indeksi (OSI) dahil olmak üzere oksidatif stres parametreleri analiz edilmiştir. AgNPs ve CP kombinasyonu, tek tek uygulamalara kıyasla hücre canlılığında önemli ölçüde daha büyük bir azalma ile sonuçlanmıştır (p<.05). CP’nin IC50 değeri AgNP’lerin varlığında azalmış ve bu da sitotoksik etkinin arttığını göstermiştir. Ek olarak, TOS ve OSI seviyelerindeki kayda değer artış ve TAS’daki düşüş, kombinasyon tedavisinin oksidatif stres aracılı hücre ölümüne neden olduğunu göstermiştir. Karaerik üzüm yapraklarından yeşil olarak sentezlenen AgNP’ler, oksidatif stres yolaklarını modüle ederek CP kaynaklı sitotoksisiteyi güçlendirmekte ve meme kanseri tedavisinde adjuvan olarak potansiyel rollerini ortaya koymaktadır. Bu bulgular, onkolojide yeşil nanoteknoloji tabanlı stratejilerin önemini vurgulamaktadır.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Tuğba Atici
0000-0002-4798-935X
Türkiye

Deniz Altun Çolak ^*
0000-0002-3576-0355
Türkiye

Early Pub Date

July 25, 2025

Publication Date

September 11, 2025

Submission Date

September 23, 2024

Acceptance Date

May 16, 2025

Published in Issue

Year 2025 Volume: 28 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1554474

IZ

https://izlik.org/JA86ZK88LR

Cite

RIS / Bibtex

APA

Atici, T., & Altun Çolak, D. (2025). Combination of Silver Nanoparticles Synthesized Using Karaerik Extract and Cisplatin: Effects on Breast Cancer Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(5), 1229-1240. https://doi.org/10.18016/ksutarimdoga.vi.1554474

Cited By

Bitki Ekstraktlarından Sentezlenen Nanopartiküllerin Domates (Solanum lycopersicum L.) Gelişimi Üzerine Etkileri

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1721574