Screening of Eggplant F3 Segregating Population for Salt Tolerance

Esra Cebeci; Hatice Filiz Boyacı; Sevinç Kıran; Şeküre Şebnem Ellialtıoğlu

doi:10.18016/ksutarimdoga.vi.1555674

TR EN

Patlıcanda F3 Kademesindeki Açılım Popülasyonunun Tuzluluk Toleransı Açısından Taranması

Abstract

Toprak tuzluluğundan etkilenen alanlarda tuza dayanıklı çeşitlerin kullanılması çevre dostu bir çözümdür. Patlıcanın yabani akrabaları bazı abiyotik streslere karşı toleransa sahiptir. Bu çalışmanın amacı, patlıcanda türler arası melez programından geliştirilen F3 popülasyonunun tuza tolerans durumunu belirlemektir. Arzu edilen özelliklere sahip bir kültür patlıcanı saf hattı BATEM-TDC47 (Solanum melongena L.) ve Solanum incanum L. arasındaki melezlemelerden geliştirilen 50 F3 hattı ve ebeveynlerinden 4-5 yapraklı büyüme aşamasındaki on dörder bitki 150 mM NaCl stresi altında test edilmiştir. Her bir hat ve ebeveyn için dörder adet bitki de kontrol olarak kullanılmıştır. Son tuz uygulamasından sonraki 12. günde bitkiler 0-5 görsel skalası kullanılarak değerlendirilmiştir. Ayrıca, stres altındaki bitkilerden alınan taze yaprak örneklerinde malondialdehit (MDA) ve prolin düzeyleri analiz edilmiştir. Yapılan gözlemler sonucu her hattan tuza en dayanıklı dört bitki seçilerek F4 jenerasyonunu üretmek için seraya transfer edilmiştir. Serada normal koşullarda yürütülen yetiştirme dönemi boyunca bitki boyu, gövde çapı, meyve rengi, meyve şekli ve antosiyanin varlığı gibi bitki ve meyve özellikleri de dâhil olmak üzere patlıcan için önemli 13 morfolojik özellik incelenmiş ve bu esnada F3 bitkileri kendilenerek F4 nesli üretilmiştir. Araştırma sonuçları değerlendirildiğinde, görsel skala ve prolin birikimlerinin bazı istisnalar dışında paralel artışlar ve azalışlar gösterdiği belirlenmiş olup, S. melongena' nın tuz toleransının arttırılması için S. incanum' un polen donörü olarak kullanılabileceği açıklanmıştır.

Keywords

Screening of Eggplant F3 Segregating Population for Salt Tolerance

Abstract

Utilizing salt-tolerant varieties in affected lands is the most prominent environmentally friendly solution. Wild relatives of eggplant have tolerance to some abiotic stresses. The aim of the study was to assess salinity tolerance in the third filial segregating population of eggplant lines that were previously associated with salt tolerance, then they could be used in breeding programs. The 50 F3 families resulting from crossings of the inbred line BATEMTDC47 (Solanum melongena L.) and Solanum incanum L. were screened under 150 mM NACl stress. A total of fourteen seedlings at the four-five leaves stage from each of the 50 F3 lines, accompanied by seedlings of two parents, were examined beside, four seedlings per line served as controls. All stressed seedlings were assessed comparatively with their controls by 0-5 visual scale, on the 12th day following the final salt treatment. Additionally, malondialdehyde (MDA) and proline levels in stressed fresh leaf samples were analyzed. The most tolerant four plantlets from each line were selected and transferred to the greenhouse to generate F4 seeds. During the greenhouse cultivation period, 13 morphological traits including plant and fruit features, such as plant height, stem diameter, anthocyanin presence, fruit color, and fruit shape etc., were studied. Following the observations, F3 plants were self-pollinated to produce F4 generation. Except for a few outliers, the visual scale and proline accumulations showed concurrent increases and reductions. Overall, the results also demonstrate that enhancement of salt tolerance of Solanum melongena can be improved using Solanum incanum as a donor of alleles.

Keywords

Supporting Institution

TAGEM

Project Number

TAGEM/BBAD/B/20/A1/P1/1476

Ethical Statement

Gerekmiyor

Thanks

This research is a part of a project titled “Development of Tolerant Inbred Lines to Salt and Drought Stresses in Eggplant through Interspecific Hybridization" and supported by Republic of Türkiye Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies (Project No: TAGEM/BBAD/B/20/A1/P1/1476)”.

References

Akinci, I. E., Akinci, S., Yilmaz, K., & Dikici, H. (2004). Response of eggplant varieties (Solanum melongena) to salinity in germination and seedling stages. New Zealand Journal of Crop and Horticultural Science, 32(2), 193-200.
Araus, J. L., & Cairns, J. E. (2014). Field high-throughput phenotyping: the new crop breeding frontier. Trends in plant science, 19(1), 52-61.
Ayranci, R., & Bagci, S. A. (2020). Utilization of Stress Tolerant Local Genotypes in Wheat Breeding Program in Context to Global Climate Change. Ekin Journal of Crop Breeding and Genetics, 6(1), 11-26.
Bates. L. S.. Waldren. R. P.. & Teare. I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39(1). 205-207.
Bekhradi, F., Delshad, M., Marín, A., Luna, M. C., Garrido, Y., Kashi, A., ... & Gil, M. I. (2015). Effects of salt stress on physiological and postharvest quality characteristics of different Iranian genotypes of basil. Horticulture, Environment, and Biotechnology, 56, 777-785.
Brenes. M.. Solana. A.. Boscaiu. M.. Fita. A.. Vicente. O.. Calatayud. Á.. ... & Plazas. M. (2020a). Physiological and biochemical responses to salt stress in cultivated eggplant (Solanum melongena L.) and in S. insanum L.. a close wild relative. Agronomy. 10(5), 651.
Brenes, M., Pérez, J., González-Orenga, S., Solana, A., Boscaiu, M., Prohens, J., ... & Vicente, O. (2020b). Comparative studies on the physiological and biochemical responses to salt stress of eggplant (Solanum melongena) and its rootstock S. torvum. Agriculture,10(8), 328.
Cebeci, E., Boyacı, H. F., Kıran, S., & Ellialtıoğlu, Ş. Ş., (2024). Assessment Results of Salinity Stressed F2 Population Originated from Interspecific Hybridization of Eggplant with Wild Relative Solanum incanum L. Horticultural Studies, 41(2), 50-59.

Chinnusamy. V., Jagendorf. A., & Zhu. J. K. (2005). Understanding and improving salt tolerance in plants. Crop Science. 45(2), 437-448.
Dadshani, S., Sharma, R. C., Baum, M., Ogbonnaya, F. C., Leon, J., & Ballvora, A. (2019). Multi-dimensional evaluation of response to salt stress in wheat. PloS one, 14(9), e0222659.
Daunay, M. C., Lester, R. N., & Laterrot, H. (1991). The use of wild species for the genetic improvement of brinjal egg-plant (Solanum melongena) and tomato (Lycopersicon esculentum). Solanaceae III; taxonomy, chemistry, evolution. https://worldveg.tind.io/record/17407/
Dasgan, H. Y., Aktas, H., Abak, K., & Cakmak, I. (2002). Determination of screening techniques to salinity tolerance in tomatoes and investigation of genotype responses. Plant Science, 163(4), 695-703.
Dejampour, J., Aliasgarzadeh, N., Grigorian, V., & Heravan, E. M. (2012). Evaluation of salinity tolerance in some interspecific hybrids of prunus. Seed and Plant Journal, 28(3), 339-351.
Dwivedi, K., Roy, A. K., Kaushal, P., Pathak, S., & Malaviya, D. R. (2022). Improving salt tolerance in Trifolium alexandrinum L. through interspecific hybridization, polyploidization and induced variations. Cytology and Genetics, 56(3), 301-311.
Genc, Y., Oldach, K., Verbyla, A. P., Lott, G., Hassan, M., Tester, M., ... & McDonald, G. K. (2010). Sodium exclusion QTL associated with improved seedling growth in bread wheat under salinity stress. Theoretical and Applied Genetics, 121, 877-894.
Gramazio, P., Blanca, J., Ziarsolo, P., Herraiz, F. J., Plazas, M., Prohens, J., & Vilanova, S. (2016). Transcriptome analysis and molecular marker discovery in Solanum incanum and S. aethiopicum, two close relatives of the common eggplant (Solanum melongena) with interest for breeding. Bmc Genomics, 17, 1-17.
Hanci, F., & Cebeci, E. (2019). Determination of morphological variability of different pisum genotypes using principal component analysis. Legume Research-An International Journal, 42(2), 162-167.
Hannachi. S., & Van Labeke. M. C. (2018). Salt stress affects germination, seedling growth and physiological responses differentially in eggplant cultivars (Solanum melongena L.). Scientia Horticulturae. 228. 56-65.
Hayat, S., Hayat, Q., Alyemeni, M. N., Wani, A. S., Pichtel, J., & Ahmad, A. (2012). Role of proline under changing environments: a review. Plant signaling & behavior, 7(11), 1456-1466.
Himabindu, Y., Chakradhar, T., Reddy, M.C., Kanygin, A., Redding, K.E., Chandrasekhar, T. (2016). Salt-Tolerant Genes from Halophytes Are Potential Key Players of Salt Tolerance in Glycophytes; Elsevier: Amsterdam, The Netherlands, Volume 124, ISBN 0114802411244.
Ivushkin. K., Bartholomeus. H., Bregt. A. K., Pulatov. A., Kempen. B., & De Sousa. L. (2019). Global mapping of soil salinity change. Remote Sensing of Environment. 231. 111260.
Lester, R. N., & Hasan, S. M. Z. (1991). Origin and domestication of the brinjal egg-plant, Solanum melogngena, from S. incanum, in Africa and Asia. Solanaceae III: taxonomy, chemistry, evolution.
Rao, C. K. (2011). Use of Brinjal (Solanum melongena L.) in alternative systems of medicine in India. FBAE, Bangalore, India.
Kilian. B., Dempewolf. H., Guarino. L., Werner. P., Coyne. C., & Warburton. M. L. (2021). Crop Science special issue: Adapting agriculture to climate change: A walk on the wild side. Crop Science. 61(1), 32-36.
Kıran, S., Kuşvuran, Ş., Özkay, F., Özgün, Ö., Sönmez, K., Özbek, H., & Ellialtıoğlu, Ş. Ş. (2015). Bazı patlıcan anaçlarının tuzluluk stresi koşullarındaki gelişmelerinin karşılaştırılması. Tarım Bilimleri Araştırma Dergisi, 8(1), 20-30.
Kuşvuran, Ş., Ellialtıoğlu, Ş., Abak, K., & Yaşar, F. (2007). Bazı kavun Cucumis sp. Genotiplerinin tuz stresine tepkileri. Journal of Agricultural Sciences, 13(04), 395-404.
Maas. E. V. (1990). Crop: In: Agricultural Salinity Assessment and Management. pp. 262–304. Tanji. K. K.. Ed.. ASCE Manuals and Reports on Engineering No. 71. New York.
Marschner, H., (1995). Saline Soils, in: Mineral Nutrition of Higher Plants, Academic Press, New York, 1995, pp. 657 /680.
Nee, M. (1999). Synopsis of Solanum in the new world. Solanaceae IV: advances in biology and utilization. Royal Botanic Gardens, Kew, 285-333.
Ortega-Albero, N., González-Orenga, S., Vicente, O., Rodríguez-Burruezo, A., & Fita, A. (2023). Responses to salt stress of the interspecific hybrid Solanum insanum× Solanum melongena and its parental species. Plants, 12(2), 295.
Plazas. M., Nguyen. H. T., González-Orenga. S., Fita. A., Vicente. O., Prohens. J., & Boscaiu. M. (2019). Comparative analysis of the responses to water stress in eggplant (Solanum melongena) cultivars. Plant Physiology and Biochemistry. 143, 72-82.
Prohens, J., Whitaker, B. D., Plazas, M., Vilanova, S., Hurtado, M., Blasco, M., ... & Stommel, J. R. (2013). Genetic diversity in morphological characters and phenolic acids content resulting from an interspecific cross between eggplant, Solanum melongena, and its wild ancestor (S. incanum). Annals of Applied Biology, 162(2), 242-257.
Saranga, Y., Cahaner, A., Zamir, D., Marani, A., & Rudich, J. (1992). Breeding tomatoes for salt tolerance: inheritance of salt tolerance and related traits in interspecific populations. Theoretical and Applied Genetics, 84, 390-396.
Shehzad, M., Zhou, Z., Ditta, A., Khan, M., Cai, X., Xu, Y., ... & Liu, F. (2021). Identification and characterization of genes related to salt stress tolerance within segregation distortion regions of genetic map in F2 population of upland cotton. PloS one, 16(3), e0247593.
Sumalan, R.M., Ciulca, S.I., Poiana, M.A., Moigradean, D., Radulov, I., Negrea, M., Crisan, M.E., Copolovici, L.; Sumalan, R.L. (2020). The Antioxidant Profile Evaluation of Some Tomato Landraces with Soil Salinity Tolerance Correlated with High Nutraceutical and Functional Value. Agronomy, 10, 500.
Tang, X., Mu, X., Shao, H., Wang, H., Brestic, M. (2015).Global plant-responding mechanisms to salt stress: Physiological and molecular levels and implications in biotechnology. Crit. Rev. Biotechnol., 35, 425–437.
Tiwari, R. S., Picchioni, G. A., Steiner, R. L., Jones, D. C., Hughs, S. E., & Zhang, J. (2013). Genetic variation in salt tolerance at the seedling stage in an interspecific backcross inbred line population of cultivated tetraploid cotton. Euphytica, 194, 1-11.
UPOV, C. (1992). International Union for the protection of new varieties of plants. https://www.upov.int/edocs/mdocs/upov/en/c_26/c_26_13.pdf
Ünlükara, A., Kurunç, A., Kesmez, G. D., Yurtseven, E., & Suarez, D. L. (2010). Effects of salinity on eggplant (Solanum melongena L.) growth and evapotranspiration. Irrigation and Drainage: The Journal of the International Commission on Irrigation and Drainage, 59(2), 203-214.
Vieira, R. A., Nogueira, A. P. O., & Fritsche-Neto, R. (2025). Optimizing the selection of quantitative traits in plant breeding using simulation. Frontiers in Plant Science, 16, 1495662.
Yaşar. F. Talhouni, M., Ellialtioglu, Ş.Ş., Kusvuran, Ş & Uzal, Ö., (2013). SOD, CAT, GR and APX enzyme activities in callus tissues of susceptible and tolerant eggplant varieties under salt stress. Research Journal of Biotechnology. 8(11), 45-51.
Ziegel, E. (2002). Editor’ s report on Encyclopaedia of Environmetrics, Vols. 1– 4, (A. El Saharawi and W. Piegorsch, Technometrics,) 44, 408– 409.

Details

Primary Language

English

Subjects

Vegetable Growing and Treatment

Journal Section

Research Article

Authors

Esra Cebeci ^*
0000-0003-0410-2453
Türkiye

Hatice Filiz Boyacı
0000-0002-3799-4673
Türkiye

Sevinç Kıran
0000-0001-6808-8326
Türkiye

Şeküre Şebnem Ellialtıoğlu
0000-0002-3851-466X
Türkiye

Early Pub Date

May 1, 2025

Publication Date

June 9, 2025

Submission Date

September 25, 2024

Acceptance Date

April 7, 2025

Published in Issue

Year 2025 Volume: 28 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1555674

IZ

https://izlik.org/JA86XC42LL

Cite

RIS / Bibtex

APA

Cebeci, E., Boyacı, H. F., Kıran, S., & Ellialtıoğlu, Ş. Ş. (2025). Screening of Eggplant F3 Segregating Population for Salt Tolerance. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(3), 746-756. https://doi.org/10.18016/ksutarimdoga.vi.1555674

AMA

1.Cebeci E, Boyacı HF, Kıran S, Ellialtıoğlu ŞŞ. Screening of Eggplant F3 Segregating Population for Salt Tolerance. KSU J. Agric Nat. 2025;28(3):746-756. doi:10.18016/ksutarimdoga.vi.1555674

Chicago

Cebeci, Esra, Hatice Filiz Boyacı, Sevinç Kıran, and Şeküre Şebnem Ellialtıoğlu. 2025. “Screening of Eggplant F3 Segregating Population for Salt Tolerance”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 28 (3): 746-56. https://doi.org/10.18016/ksutarimdoga.vi.1555674.

EndNote

Cebeci E, Boyacı HF, Kıran S, Ellialtıoğlu ŞŞ (June 1, 2025) Screening of Eggplant F3 Segregating Population for Salt Tolerance. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28 3 746–756.

IEEE

[1]E. Cebeci, H. F. Boyacı, S. Kıran, and Ş. Ş. Ellialtıoğlu, “Screening of Eggplant F3 Segregating Population for Salt Tolerance”, KSU J. Agric Nat., vol. 28, no. 3, pp. 746–756, June 2025, doi: 10.18016/ksutarimdoga.vi.1555674.

ISNAD

Cebeci, Esra - Boyacı, Hatice Filiz - Kıran, Sevinç - Ellialtıoğlu, Şeküre Şebnem. “Screening of Eggplant F3 Segregating Population for Salt Tolerance”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28/3 (June 1, 2025): 746-756. https://doi.org/10.18016/ksutarimdoga.vi.1555674.

JAMA

1.Cebeci E, Boyacı HF, Kıran S, Ellialtıoğlu ŞŞ. Screening of Eggplant F3 Segregating Population for Salt Tolerance. KSU J. Agric Nat. 2025;28:746–756.

MLA

Cebeci, Esra, et al. “Screening of Eggplant F3 Segregating Population for Salt Tolerance”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 28, no. 3, June 2025, pp. 746-5, doi:10.18016/ksutarimdoga.vi.1555674.

Vancouver

1.Esra Cebeci, Hatice Filiz Boyacı, Sevinç Kıran, Şeküre Şebnem Ellialtıoğlu. Screening of Eggplant F3 Segregating Population for Salt Tolerance. KSU J. Agric Nat. 2025 Jun. 1;28(3):746-5. doi:10.18016/ksutarimdoga.vi.1555674