Screening of Eggplant F3 Segregating Population for Salt Tolerance

Esra Cebeci; Hatice Filiz Boyacı; Sevinç Kıran; Şeküre Şebnem Ellialtıoğlu

doi:10.18016/ksutarimdoga.vi.1555674

TR EN

Patlıcanda F3 Kademesindeki Açılım Popülasyonunun Tuzluluk Toleransı Açısından Taranması

Öz

Toprak tuzluluğundan etkilenen alanlarda tuza dayanıklı çeşitlerin kullanılması çevre dostu bir çözümdür. Patlıcanın yabani akrabaları bazı abiyotik streslere karşı toleransa sahiptir. Bu çalışmanın amacı, patlıcanda türler arası melez programından geliştirilen F3 popülasyonunun tuza tolerans durumunu belirlemektir. Arzu edilen özelliklere sahip bir kültür patlıcanı saf hattı BATEM-TDC47 (Solanum melongena L.) ve Solanum incanum L. arasındaki melezlemelerden geliştirilen 50 F3 hattı ve ebeveynlerinden 4-5 yapraklı büyüme aşamasındaki on dörder bitki 150 mM NaCl stresi altında test edilmiştir. Her bir hat ve ebeveyn için dörder adet bitki de kontrol olarak kullanılmıştır. Son tuz uygulamasından sonraki 12. günde bitkiler 0-5 görsel skalası kullanılarak değerlendirilmiştir. Ayrıca, stres altındaki bitkilerden alınan taze yaprak örneklerinde malondialdehit (MDA) ve prolin düzeyleri analiz edilmiştir. Yapılan gözlemler sonucu her hattan tuza en dayanıklı dört bitki seçilerek F4 jenerasyonunu üretmek için seraya transfer edilmiştir. Serada normal koşullarda yürütülen yetiştirme dönemi boyunca bitki boyu, gövde çapı, meyve rengi, meyve şekli ve antosiyanin varlığı gibi bitki ve meyve özellikleri de dâhil olmak üzere patlıcan için önemli 13 morfolojik özellik incelenmiş ve bu esnada F3 bitkileri kendilenerek F4 nesli üretilmiştir. Araştırma sonuçları değerlendirildiğinde, görsel skala ve prolin birikimlerinin bazı istisnalar dışında paralel artışlar ve azalışlar gösterdiği belirlenmiş olup, S. melongena' nın tuz toleransının arttırılması için S. incanum' un polen donörü olarak kullanılabileceği açıklanmıştır.

Anahtar Kelimeler

Screening of Eggplant F3 Segregating Population for Salt Tolerance

Abstract

Utilizing salt-tolerant varieties in affected lands is the most prominent environmentally friendly solution. Wild relatives of eggplant have tolerance to some abiotic stresses. The aim of the study was to assess salinity tolerance in the third filial segregating population of eggplant lines that were previously associated with salt tolerance, then they could be used in breeding programs. The 50 F3 families resulting from crossings of the inbred line BATEMTDC47 (Solanum melongena L.) and Solanum incanum L. were screened under 150 mM NACl stress. A total of fourteen seedlings at the four-five leaves stage from each of the 50 F3 lines, accompanied by seedlings of two parents, were examined beside, four seedlings per line served as controls. All stressed seedlings were assessed comparatively with their controls by 0-5 visual scale, on the 12th day following the final salt treatment. Additionally, malondialdehyde (MDA) and proline levels in stressed fresh leaf samples were analyzed. The most tolerant four plantlets from each line were selected and transferred to the greenhouse to generate F4 seeds. During the greenhouse cultivation period, 13 morphological traits including plant and fruit features, such as plant height, stem diameter, anthocyanin presence, fruit color, and fruit shape etc., were studied. Following the observations, F3 plants were self-pollinated to produce F4 generation. Except for a few outliers, the visual scale and proline accumulations showed concurrent increases and reductions. Overall, the results also demonstrate that enhancement of salt tolerance of Solanum melongena can be improved using Solanum incanum as a donor of alleles.

Keywords

Supporting Institution

TAGEM

Project Number

TAGEM/BBAD/B/20/A1/P1/1476

Ethical Statement

Gerekmiyor

Thanks

This research is a part of a project titled “Development of Tolerant Inbred Lines to Salt and Drought Stresses in Eggplant through Interspecific Hybridization" and supported by Republic of Türkiye Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies (Project No: TAGEM/BBAD/B/20/A1/P1/1476)”.

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Details

Primary Language

English

Subjects

Vegetable Growing and Treatment

Journal Section

Research Article

Authors

Esra Cebeci ^*
0000-0003-0410-2453
Türkiye

Hatice Filiz Boyacı
0000-0002-3799-4673
Türkiye

Sevinç Kıran
0000-0001-6808-8326
Türkiye

Şeküre Şebnem Ellialtıoğlu
0000-0002-3851-466X
Türkiye

Early Pub Date

May 1, 2025

Publication Date

June 9, 2025

Submission Date

September 25, 2024

Acceptance Date

April 7, 2025

Published in Issue

Year 2025 Volume: 28 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1555674

IZ

https://izlik.org/JA86XC42LL

Cite

RIS / Bibtex

APA

Cebeci, E., Boyacı, H. F., Kıran, S., & Ellialtıoğlu, Ş. Ş. (2025). Screening of Eggplant F3 Segregating Population for Salt Tolerance. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(3), 746-756. https://doi.org/10.18016/ksutarimdoga.vi.1555674