Analysis of Flavonoids Structural Genes in Between Chalaza and Microphyll a Mutant Natural Green Cotton Fiber

Öner Canavar; Hatice Kübra Gören

doi:10.18016/ksutarimdoga.vi.1575961

EN TR

Analysis of Flavonoids Structural Genes in Between Chalaza and Microphyll a Mutant Natural Green Cotton Fiber

Abstract

Undesirable coloring pigments in naturally colored fibers can affect their aesthetic and commercial value in the textile industry. In our green cotton breeding program, we identified a gene mutation that causes different colored fibers to form on a single seed. We investigated the expression patterns of flavonoid biosynthesis structural genes to understand the formation of these different colors between the chalazal and microphyll parts of the seed. Significant variations in gene expression levels were observed among the examined flavonoid genes, highlighting the complexity of flavonoid biosynthesis pathways in cotton seeds. In the green fibers on the microphyll part, lower expression levels of enzymes such as 4Cl (4-coumarate: CoA ligase), C4h (cinnamate 4-hydroxylase), F3h (flavone 3-hydroxylase), F3′5′h (flavonoid 3′5′-hydroxylase), Ans (anthocyanidin synthase), Anr (anthocyanidin reductase), and Ufgt (UDP-glucose: flavonoid 3-O-glucosyltransferase) were identified as potential factors influencing fiber coloration. Conversely, in the white fibers on the chalazal part, the expression levels of Chs (chalcone synthase) and Chı (chalcone isomerase) genes were lower than those in the microphyll part. This low expression is thought to be due to a mutation at the beginning of the phenylalanine pathway, preventing the formation of a green color in the white fibers on the chalazal part together with the low synthesis of the Chı gene. Understanding the molecular mechanisms behind these mutations is crucial for developing strategies to mitigate their effects and sustain the textile industry. The findings can inform cotton breeding programs to address unwanted coloration issues through genetic interventions, potentially enhancing the aesthetic and commercial value of naturally colored cotton fibers.

Keywords

Mutant Doğal Yeşil Pamuk Lifinde Chalaza ve Microphyll Arasındaki Flavonoid Yapısal Genlerı̇n Analizi

Öz

Doğal renkli liflerdeki istenmeyen farklı renk pigmentleri, tekstil endüstrisindeki estetik ve ticari değerlerini etkileyebilir. Yeşil pamuk ıslah programımızda, tek bir tohumunda farklı renkli liflerin oluşmasına neden olan bir gen mutasyonu tespit ettik. Tohumun şalazal ve mikrofil kısımları arasındaki bu farklı renklerin oluşumunu anlamak için flavonoid biyosentez yapısal genlerinin ifade modellerini araştırdık. İncelenen flavonoid genleri arasında gen ekspresyon seviyelerinde önemli farklılıklar gözlenmiş, bu da pamuk tohumlarındaki flavonoid biyosentez yollarının karmaşıklığını vurgulamıştır. Mikrofil kısmındaki yeşil liflerde, 4Cl (4-coumarate: CoA ligaz), C4h (sinamat 4-hidroksilaz), F3h (flavon 3-hidroksilaz), F3′5′h (flavonoid 3′5′-hidroksilaz), Ans (antosiyanidin sentaz), Anr (antosiyanidin redüktaz) ve Ufgt (UDP-glukoz: flavonoid 3-O-glukosiltransferaz) lif renklenmesini etkileyen potansiyel faktörler olarak tanımlanmıştır. Buna karşılık, şalazal kısımdaki beyaz liflerde, Chs (chalcone synthase) ve Chı (chalcone isomerase) genlerinin ifade seviyeleri mikrofil kısımdakilerden daha düşüktü. Bu düşük ifadenin, şalazal kısımdaki beyaz liflerde yeşil renk oluşumunu engelleyen fenilalanin yolunun başlangıcındaki bir mutasyondan kaynaklandığı düşünülmektedir. Bu mutasyonların arkasındaki moleküler mekanizmaları anlamak, etkilerini azaltmak ve tekstil endüstrisini sürdürmek için stratejiler geliştirmek açısından çok önemlidir. Bulgular, genetik müdahaleler yoluyla istenmeyen renklenme sorunlarını ele almak için pamuk ıslah programlarını bilgilendirebilir ve potansiyel olarak doğal renkli pamuk liflerinin estetik ve ticari değerini artırabilir.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Industrial Crops

Journal Section

Research Article

Authors

Öner Canavar
0000-0003-4168-953X
Türkiye

Hatice Kübra Gören ^*
0000-0001-7654-1450
Türkiye

Early Pub Date

January 31, 2025

Publication Date

February 12, 2025

Submission Date

October 30, 2024

Acceptance Date

December 30, 2024

Published in Issue

Year 2025 Volume: 28 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1575961

IZ

https://izlik.org/JA88HF68WK

Cite

RIS / Bibtex

APA

Canavar, Ö., & Gören, H. K. (2025). Analysis of Flavonoids Structural Genes in Between Chalaza and Microphyll a Mutant Natural Green Cotton Fiber. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(1), 191-204. https://doi.org/10.18016/ksutarimdoga.vi.1575961