Investigation of Possible Genotoxicity of Surface and Tap Waters Using the Comet Assay

Derya Bostancı; Serdar Yedıer; Seda Kontaş Yalçınkaya

doi:10.18016/ksutarimdoga.vi.1581657

EN TR

Investigation of Possible Genotoxicity of Surface and Tap Waters Using the Comet Assay

Abstract

We tested the hypothesis that chronic exposure to total heavy metal loads in Cevizdere surface water and Ünye District tap water of Ordu Province (Türkiye) may cause a genetic toxic effect in erythrocyte cells of carp (Cyprinus carpio) individuals. For this purpose, total heavy metal (loid)s contents in the surface and tap waters were determined seasonally. It was determined that both Cevizdere surface water and Ünye District tap water caused DNA damage in erythrocyte cells of carp fish, and this damage was statistically significant when compared to control groups (P<0.05). As a result of the comet analysis, the DNA damage detected in the erythrocyte cells of C. carpio showed the presence of genotoxic potential of Cevizdere Stream surface water and Ünye District tap water. The genotoxicity of the surface waters of the Cevizdere Stream, which is the main source of drinking water for the Ünye District in Ordu City, as well as the Ünye District tap waters, was evaluated in vivo for the first time using the Comet assay. This study revealed genotoxic damage in C. carpio due to total heavy metal(loid) pollution in the surface water of Cevizdere Stream and tap water of Ünye District, contributing to a better understanding of the relationship between genotoxicity and heavy metal(loid) pollution.

Keywords

Comet Analizi Kullanılarak Yüzey ve Musluk Sularının Olası Genotoksisitesinin İncelenmesi

Öz

Ordu İli (Türkiye) Cevizdere yüzey suyu ve Ünye İlçesi musluk suyundaki toplam ağır metal yüklerine kronik maruz kalmanın sazan (Cyprinus carpio) bireylerinin eritrosit hücrelerinde genetik toksik etkiye neden olabilme hipotezini test ettik. Bu amaç doğrultusunda yüzey ve musluk sularındaki toplam ağır metal(loid) içerikleri mevsimsel olarak belirlenmiştir. Hem Cevizdere yüzey suyunun hem de Ünye İlçesi musluk suyunun sazan balıklarının eritrosit hücrelerinde DNA hasarına neden olduğu ve bu hasarın kontrol gruplarıyla karşılaştırıldığında istatistiksel olarak anlamlı olduğu belirlenmiştir (P<0,05). Comet analizi sonucunda C. carpio'nun eritrosit hücrelerinde tespit edilen DNA hasarı, Cevizdere yüzey suyu ve Ünye İlçesi musluk suyunun genotoksik potansiyelinin varlığını göstermiştir. Ordu İli, Ünye İlçesi'nin içme suyunun ana kaynağı olan Cevizdere'nin yüzey sularının ve Ünye İlçesi musluk sularının genotoksisitesi Comet yöntemi kullanılarak ilk kez in vivo olarak değerlendirilmiştir. Bu çalışma, Cevizdere yüzey suyu ve Ünye İlçesi musluk suyunda toplam ağır metal(loid) kirliliğine bağlı olarak C. carpio'da genotoksik hasar olduğunu ortaya koyarak, genotoksisite ile ağır metal(loid)ler arasındaki ilişkinin daha iyi anlaşılmasına katkı sağlamıştır.

Anahtar Kelimeler

Supporting Institution

Scientific Research Projects Commission of Ordu University

Project Number

BAP; G-2004

Ethical Statement

The experimental procedures of the study were approved by the Animal Experiments Local Ethics Committee, Ordu University (Approval number: 82678388/5).

Thanks

The authors would like to thank Prof. Dr. Nazmi POLAT, Prof. Dr. Evren TUNCA, and Prof. Dr. Salih ALKAN for their valuable comments on this study.

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Details

Primary Language

English

Subjects

Aquatic Toxicology , Fish Biology

Journal Section

Research Article

Authors

Derya Bostancı ^*
0000-0003-3052-9805
Türkiye

Serdar Yedıer
0000-0003-0017-3502
Türkiye

Seda Kontaş Yalçınkaya
0000-0002-6582-6722
Türkiye

Early Pub Date

May 1, 2025

Publication Date

June 9, 2025

Submission Date

November 21, 2024

Acceptance Date

March 27, 2025

Published in Issue

Year 2025 Volume: 28 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1581657

IZ

https://izlik.org/JA75SD68KA

Cite

RIS / Bibtex

APA

Bostancı, D., Yedıer, S., & Kontaş Yalçınkaya, S. (2025). Investigation of Possible Genotoxicity of Surface and Tap Waters Using the Comet Assay. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(3), 863-873. https://doi.org/10.18016/ksutarimdoga.vi.1581657