LC-MS/MS Analysis and Biological Activities of Different Parts of Ziziphora capitate L.

Serkan Yiğitkan; Mehmet Çavuşoğlu; Mehmet Veysi Çağlayan; İsmail Yener; Mehmet Fırat; Eda Çavuş Kaya; Mustafa Abdullah Yılmaz; Abdulselam Ertaş

doi:10.18016/ksutarimdoga.vi.1585230

EN TR

LC-MS/MS Analysis and Biological Activities of Different Parts of Ziziphora capitate L.

Abstract

The Ziziphora species, classified under the Lamiaceae family, have a strong aromatic property. Ziziphora species have been used in folk medicine as sedative, gastric, aphrodisiac, bloating, and degassing. In the current study, the phenolic and flavanoid content of ethanol extracts of Ziziphora capitata L. species of flower, leaf, branch, mixed, and root parts was determined by the LC-MS/MS device. In addition, the antioxidant and cytotoxic activities of the extracts, as well as their inhibitory effects on enzymes (antihypertensive, AchE (acetylcholinesterase), BchE (butyrylcholinesterase), elastase, tyrosinase, collagenase and urease), were determined. The LC-MS/MS results showed that quinic acid (25578, 5842, 25171, 14055, 10597 µg g-1, respectively) was found in higher amounts in flower, leaf, branch, mixed, and root extracts of Z. capitata species compared to other components. Additionally, rosmarinic acid (17097 µg g-1), cynaroside (8432), and hesperidin (8067) were found to be major components. It was observed that the flower extract of the species exhibited strong antioxidant activity (IC50: 37.18±1.36 µg mL-1, 9.89±0.45, A0.5:16.27±0.02, respectively) in DPPH, ABTS and CUPRAC methods. It was concluded that the leaf extract of Z. capitata species had a strong cytotoxic effect on HT-29 (colon cancer cell line) (viability %: 9.26±0.69). It was observed that the root part of the species exhibited higher activity in butyrylcholinesterase (BChE) enzyme inhibition activity (inhibition %: 40.56±0.88) than other parts. It was determined that Z. capitata extracts did not show acetylcholinesterase, urease, tyrosinase, elastase, collagenase, and antihypertensive enzyme activity or showed low activity. As a result, it is thought that the flower extract of the Z. capitata species has better results in terms of the examined parameters, whereas the leaf extract needs to be subjected to more detailed in vitro and in vivo research conducted to be used in the pharmaceutical industry as a result of its cytotoxic effect against colon cancer cell lines.

Keywords

Ziziphora capitata L. Türünün Farklı Kısımlarının LC-MS/MS Analizi ve Biyolojik Aktiviteleri

Öz

Lamiaceae familyasının altında sınıflandırılan Ziziphora türleri güçlü aromatik özelliğe sahiptir. Halk hekimliğinde Ziziphora türleri yatıştırıcı, midevi, afrodizyak, şişkinlik ve gaz giderici olarak kullanılmıştır. Mevcut çalışmada Ziziphora capitata L. türünün çiçek, yaprak, dal, karışık ve kök kısmlarının etanol ekstrelerinin fenolik ve flavanoid içeriği LC-MS/MS cihazı ile belirlenmiştir. Ayrıca ekstrelerin antioksidan, sitotoksik etkileri ile enzim inhibisyon aktiviteleri (antihipertansif, AchE (asetilkolinesteraz), BchE (bütirilkolinesteraz), elastaz, tirozinaz, kollajenaz ve üreaz) belirlenmiştir. LC-MS/MS sonuçlarına göre Z. capitata türünün çiçek, yaprak, dal, karışık ve kök ekstrelerinde kinik asidin (sırasıyla, 25578, 5842, 25171, 14055, 10597 µg g-1,) diğer bileşenlerden daha yüksek içeriğe sahip olduğu tespit edilmiştir. Ayrıca rosmarinik asit (17097 µg g-1), sinarozit (8432) ve hesperidin (8067) bileşiklerinin majör bileşenler olduğu bulunmuştur. DPPH, ABTS ve CUPRAC yöntemlerinde türün çiçek ekstresinin güçlü antioksidan aktivitesi (sırasıyla: IC50: 37.18±1.36 µg mL-1; 9.89±0.45; A0.5:16.27±0.02) sergilediği görülmüştür. Z. capitata türünün yaprak ekstresinin HT-29 (kolon kanseri hücre hattı) üzerine (% canlılık: 9.26±0.69) güçlü sitotoksik etki gösterdiği belirlenmiştir. Bütirilkolinesteraz (BChE) enzim inhibisyon aktivitesinde türün kök kısmının (%inhibison: 40.56±0.88) diğer kısımlarından daha yüksek aktivite sergilediği görülmüştür. Z. capitata türünün etanol ekstrelerinin asetilkolinesteraz, üreaz, tirozinaz, elastaz, kollajenaz ve antihipertansif enzim aktivitesi göstermediği veya düşük aktivite gösterdiği belirlenmiştir. Sonuç olarak, Z. capitata türünün çiçek ekstresinin incelenen parametreler açısından daha iyi sonuçlara sahip olduğu, yaprak ekstresinin ise kolon kanseri hücre hattı üzerindeki sitotoksik etkisinden dolayı türün ilaç endüstürisinde kullanılabilmesi için in vitro ve in vivo olarak detaylı araştırmalara tabi tutulması gerektiği düşünülmektedir.

Anahtar Kelimeler

Supporting Institution

Dicle Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

ECZACILIK.24.007 ve FEN.24.005

Ethical Statement

Bu çalışma, sadece bitkisel örneklerin toplanması ve bitkisel bileşiklerin kimyasal analizlerinin yapılması üzerine odaklanmıştır. İnsan ya da hayvan denekleri kullanılmadığı ve herhangi bir etik müdahale gerektiren deneysel süreç gerçekleştirilmediği için, etik kurul izni gerektirmemektedir.

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Details

Primary Language

English

Subjects

Plant Biochemistry

Journal Section

Research Article

Authors

Serkan Yiğitkan ^*
0000-0002-6202-1515
Türkiye

Mehmet Çavuşoğlu
0000-0002-8871-5646
Türkiye

Mehmet Veysi Çağlayan
0000-0002-9803-6444
Türkiye

İsmail Yener
0000-0002-0988-9462
Türkiye

Mehmet Fırat
0000-0001-5814-614X
Türkiye

Eda Çavuş Kaya
0000-0002-5359-8056
Türkiye

Mustafa Abdullah Yılmaz
0000-0002-4090-7227
Türkiye

Abdulselam Ertaş
0000-0002-2193-8386
Türkiye

Early Pub Date

December 26, 2024

Publication Date

December 31, 2024

Submission Date

November 14, 2024

Acceptance Date

December 25, 2024

Published in Issue

Year 2024 Volume: 27 Number: Ek Sayı 2 (Suppl 2)

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1585230

IZ

https://izlik.org/JA89CH34NR

Cite

RIS / Bibtex

APA

Yiğitkan, S., Çavuşoğlu, M., Çağlayan, M. V., Yener, İ., Fırat, M., Çavuş Kaya, E., Yılmaz, M. A., & Ertaş, A. (2024). LC-MS/MS Analysis and Biological Activities of Different Parts of Ziziphora capitate L. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(Ek Sayı 2 (Suppl 2), 307-315. https://doi.org/10.18016/ksutarimdoga.vi.1585230

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