Morphology and Molecular Phylogeny of Blepharisma hyalinum Perty, 1849 (Ciliophora, Heterotrichida) Isolated from Soil

Naciye Gülkız Şenler; İsmail Yıldız; Hilal Ural; Rıfat Bircan

doi:10.18016/ksutarimdoga.vi.1590418

EN TR

Morphology and Molecular Phylogeny of Blepharisma hyalinum Perty, 1849 (Ciliophora, Heterotrichida) Isolated from Soil

Abstract

In this study, two successive populations of Blepharisma were observed in cultures prepared from soil samples collected from Tekirdağ (Türkiye). The cells and cysts belonging to these populations were examined morphologically and morphometrically, and their phylogenetic positions were determined based on SSU rDNA gene sequences. Both populations were morphometrically very similar to each other, but population 1, the initial population observed in soil cultures, had a pyriform body that was inflexible and rigid while population 2, observed subsequent to the disappearance of population 1, exhibited a flexible, fragile, lanceolate body. In addition, the cell width/length ratios of both populations were found to be statistically different from each other. However, since the SSU rDNA nucleotide sequences of both populations are very similar to each other and to other B. hyalinum populations described previously, it is concluded that the observed Blepharisma populations represent different morphotypes of B. hyalinum. It has been hypothesised that the observed morphological differences may result from diet, environmental factors, and variations in the life stages of the cultured cells. Comparison of SSU rDNA nucleotide sequences of Blepharisma populations and phylogenetic analyses showed that the nucleotide sequences of some different species are identical or very similar. To clarify this chaotic situation, extensive molecular data based on detailed morphological studies on Blepharisma populations are necessary.

Keywords

Topraktan İzole Edilen Blepharisma hyalinum Perty, 1849 (Ciliophora, Heterotrichida) Türünün Morfolojisi ve Moleküler Filogenisi

Öz

Bu çalışmada Tekirdağ (Türkiye) topraklarından hazırlanan kültürlerde birbiri ardınca ortaya çıkan iki Blepharisma popülasyonu gözlendi. Bu popülasyonlara ait hücre ve kistler morfolojik ve morfometrik olarak incelenmiş ve SSU rDNA gen dizisine dayanarak filogenetik konumları belirlenmiştir. Her iki popülasyon da morfometrik olarak birbirine çok benzerdi, ancak toprak kültürlerinde gözlenen ilk popülasyon olan popülasyon 1, esnek olmayan ve sert olan piriform biçime sahipken, popülasyon 1'in kaybolmasının ardından gözlenen popülasyon 2 ise, esnek, kırılgan, mızrak biçimindedir. Ayrıca, her iki popülasyonun hücre genişliği/uzunluğu oranlarının istatistiksel olarak birbirinden farklı olduğu bulunmuştur. Bununla birlikte, her iki popülasyonun SSU rDNA nükleotid dizileri birbirlerine ve daha önce tanımlanan diğer B. hyalinum popülasyonlarına çok benzediğinden, bu çalışmada gözlemlenen Blepharisma popülasyonlarının B. hyalinum'un morfotipleri olduğu sonucuna varıldı. Gözlenen morfolojik farkların çevresel faktörlere ve kültürlerdeki hücrelerin farklı yaşam evrelerinden kaynaklanabileceği varsayılmıştır.Blepharisma popülasyonlarının SSU rDNA nükleotid dizilerinin karşılaştırılması ve filogenetik analizler bazı farklı türlere ait nükleotid dizilerinin aynı ya da çok benzer olduklarını göstermiştir. Bu kaotik durumun açıklığa kavuşturulması için Blepharisma popülasyonları üzerinde ayrıntılı morfolojik incelemelere dayalı çok sayıda moleküler veriye ihtiyaç duyulmaktadır.

Anahtar Kelimeler

Project Number

TÜBİTAK 221Z208

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Details

Primary Language

English

Subjects

Microbial Genetics , Microbiology (Other)

Journal Section

Research Article

Authors

Naciye Gülkız Şenler
0000-0002-5035-0880
Türkiye

İsmail Yıldız ^*
0000-0001-9986-0358
Türkiye

Hilal Ural
0009-0004-9928-7626
Türkiye

Rıfat Bircan
0000-0001-5291-8620
Türkiye

Early Pub Date

June 11, 2025

Publication Date

July 4, 2025

Submission Date

November 25, 2024

Acceptance Date

May 21, 2025

Published in Issue

Year 2025 Volume: 28 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1590418

IZ

https://izlik.org/JA97JN83TC

Cite

RIS / Bibtex

APA

Şenler, N. G., Yıldız, İ., Ural, H., & Bircan, R. (2025). Morphology and Molecular Phylogeny of Blepharisma hyalinum Perty, 1849 (Ciliophora, Heterotrichida) Isolated from Soil. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 914-929. https://doi.org/10.18016/ksutarimdoga.vi.1590418