Phytochemical Analysis of Pyracantha angustifolia: Synthesis of Silver Nanoparticles, Characterization, Antimicrobial Activity, and In Silico Studies

Yunus Başar; Aybek Yiğit; Mesude Figen Dönmez; İbrahim Demirtas; Mehmet Hakkı Alma

doi:10.18016/ksutarimdoga.vi.1593890

TR EN

Pyracantha angustifolia'nın Fitokimyasal Analizi: Gümüş Nanopartiküllerinin Sentezi, Karakterizasyonu, Antimikrobiyal Aktivitesi Ve In Silico Çalışmaları

Öz

Nanoteknoloji çeşitli endüstrilerde hızla ilerlemekte ve çevre dostu nanopartikül (NP) sentez yöntemlerine olan ihtiyacı vurgulamaktadır. Bu çalışmada, gıda ve ilaçlarda uygulamaları olan bir bitki olan ateş dikeni olarak da bilinen Pyracantha angustifolia (PA) kullanılarak gümüş nanopartiküller (AgNP'ler) sentezlendi. AgNP'ler UV, FT-IR, FE-SEM, FESEM-EDX ile karakterize edilmiş ve antibakteriyel aktiviteleri Erwinia caratovora ve Clavibacter michiganensis'e karşı test edilmiştir. PA sulu özütünün fitokimyasal analizi, sırasıyla LC-ESI-MS/MS ve GC-MS/MS kullanılarak hesperidin (1240,3564 ng/ml) ve palmitik asit metil esteri (%30,79) ana bileşenler olarak tanımlandı. PA'nın AgNP'leri E. caratovora ve C. michiganensis patojenlerine karşı yüksek antibakteriyel aktivite gösterdi. İn silico çalışmalar hespiridinin DNA giraz ile etkileşimini doğruladı (MolDock skoru; -123,43, bağlanma enerjileri; -7,00 kcal/mol ve ΔG bağlanma değerleri; 15,051 kj/mol), bu da onun antibakteriyel ajan olarak potansiyelini destekledi.

Anahtar Kelimeler

Phytochemical Analysis of Pyracantha angustifolia: Synthesis of Silver Nanoparticles, Characterization, Antimicrobial Activity, and In Silico Studies

Abstract

Nanotechnology is rapidly advancing across various industries, highlighting the need for environmentally friendly nanoparticle (NP) synthesis methods. In this study, silver nanoparticles (AgNPs) were synthesized using Pyracantha angustifolia (PA), also known as firethorn, a plant with applications in food and pharmaceuticals. The AgNPs were characterized via UV-VIS, FT-IR, FE-SEM, FESEM-EDX, and their antibacterial activity was tested against Erwinia caratovora and Clavibacter michiganensis. Phytochemical analysis of PA aqueous extract identified hesperidin (1240.3564 ng/ml) and palmitic acid methyl ester (30.79%) as major components using LC-ESI-MS/MS and GC-MS/MS, respectively. AgNPs of PA showed high antibacterial activity against the pathogens E. caratovora and C. michiganensis. In silico studies confirmed hespiridin’s interaction with DNA gyrase (MolDock score; -123.43, binding energies; -7.00 kcal/mol and ΔG binding values; 15.051 kj/mol), supporting its potential as an antibacterial agent.

Keywords

Project Number

YİP0723İ01

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Details

Primary Language

English

Subjects

Plant Biochemistry , Food Engineering

Journal Section

Research Article

Authors

Yunus Başar ^*
0000-0002-7785-3242
Türkiye

Aybek Yiğit
0000-0001-8279-5908
Türkiye

Mesude Figen Dönmez
0000-0002-7992-8252
Türkiye

İbrahim Demirtas
0000-0001-8946-647X
Türkiye

Mehmet Hakkı Alma
0000-0001-6323-7230
Türkiye

Early Pub Date

October 18, 2025

Publication Date

January 1, 2026

Submission Date

November 30, 2024

Acceptance Date

July 11, 2025

Published in Issue

Year 2026 Volume: 29 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1593890

IZ

https://izlik.org/JA75CF85RJ

Cite

RIS / Bibtex

APA

Başar, Y., Yiğit, A., Dönmez, M. F., Demirtas, İ., & Alma, M. H. (2026). Phytochemical Analysis of Pyracantha angustifolia: Synthesis of Silver Nanoparticles, Characterization, Antimicrobial Activity, and In Silico Studies. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 29(1), 1-14. https://doi.org/10.18016/ksutarimdoga.vi.1593890

Cited By

Green Synthesis of Silver Nanoparticles from Stachys setifera subsp. lycia: Characterization, Antioxidant Activity, and In Silico Studies

Chemistry & Biodiversity

https://doi.org/10.1002/cbdv.202503467