Influences of Light-Emitting Diodes (LEDs) and Culture Media on Micropropagation of Native Hemp (Cannabis sativa) Population from Türkiye

Cennet Yaman; Güngör Yılmaz; Serkan Uranbey

doi:10.18016/ksutarimdoga.vi.1596868

TR EN

Türkiye'deki Yerel Kenevir (Cannabis sativa) Popülasyonunun Mikroçoğaltımı Üzerinde Işık Yayan Diyotların (LED'ler) ve Kültür Ortamlarının Etkileri

Öz

Işık yayan diyotlar (LED'ler), tarihsel olarak serada endüstriyel kenevir (Cannabis sativa) yetiştiriciliğinde ek aydınlatma için standart olmuştur, ancak C. sativa'da in vitro koşullarında çeşitli LED'ler altında farklı ortamlar konusunda bilimsel anlayış eksikliği vardır. Bu çalışmanın amacı, C. sativa'nın mikro çoğaltılması için en iyi performans gösteren LED'leri ve besin ortamlarını belirlemektir. Bu çalışmada, sabit ışık fotoperiyodu korunarak iki farklı spektrum (beyaz ve kırmızı LED'ler) ve farklı ortamlar [MS, B5, MS + B5, ½ MS, ½ B5, ½ MS + ½ B5 ve kontrol (ortamsız)] kullanıldı. Işık spektrumlarının ve besin ortamlarının çeşitliliği, C. sativa'nın büyüme paarametreleri için istatistiksel olarak anlamlı farklılıklar gösterdi. Kırmızı LED, 7,37 cm ile olağanüstü yüksek bir sürgün uzunluğu sağlarken, beyaz LED 6,57 cm ile en yüksek kök uzunluğunu sergiledi. Ayrıca, besin ortamlarının tam güçteki ortamlarda sürgün büyümesinde, yarı güçteki ortamlarda ise kök büyümesinde etkili olduğu bulunmuştur. Bitki büyüme parametreleri arasında, bitki başına sürgün ve nod sayıları klonal çoğaltım için büyük önem taşımaktadır. Kırmızı LED altında B5 ortamı 4.64 ile olağanüstü yüksek bir sürgün sayısı sağlarken, beyaz LED altında MS ortamı 5.87 ile en yüksek nod sayısını sergilemiştir. İstenilen parametrelerin en iyi sonuçları için her bir LED kaynağı ilgili ortam ayarlamasını gerektirmiştir. Bu açıdan, çalışma C. sativa'nın mikroçoğaltımında uygun besin ortamının ve LED spektrumunun seçilmesi için avantajlı olabilir.

Anahtar Kelimeler

Influences of Light-Emitting Diodes (LEDs) and Culture Media on Micropropagation of Native Hemp (Cannabis sativa) Population from Türkiye

Abstract

Light-emitting diodes (LEDs) have historically been the standard for supplemental lighting in industrial hemp (Cannabis sativa) cultivation in a greenhouse, but there is a lack of scientific understanding in C. sativa regarding different media under various LEDs in vitro conditions. The aim of this study was to determine the top-performing LEDs and nutrient media for micropropagation of C. sativa. In this study, two different spectra (white and red LEDs) and different media [MS, B5, MS+B5, ½ MS, ½ B5, ½ MS + ½ B5, and control (without medium)] were used, maintaining constant light photoperiod. The diversity of light spectra and nutrient media showed statistically significant differences for the growth parameters of C. sativa. Red LED provided an extraordinarily high shoot length of 7.37 cm, whereas white LED exhibited the highest root length with 6.57 cm. Also, nutrient media were found to be effective on shoot growth in full-strength media, while on root growth in half-strength media. Among the plant growth parameters, the number of shoots and nodes per plant is of great importance for clonal propagation. B5 medium under red LED provided an extraordinarily high shoot number with 4.64, whereas MS medium under white LED exhibited the highest node number with 5.87. Each LED source required the respective medium adjustment for the best results of the desired parameters. In this respect, the study may be advantageous for the selection of the appropriate nutrient medium and led spectrum in micropropagation of C. sativa.

Keywords

Ethical Statement

The authors of the articles declare that they have no conflict of interest.

Thanks

The authors are grateful to the Hemp Research Institute of Yozgat Bozok University in Türkiye for helping to procure the seeds.

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Details

Primary Language

English

Subjects

Plant Biotechnology , Agricultural Biotechnology (Other)

Journal Section

Research Article

Authors

Cennet Yaman ^*
0000-0002-2364-8171
Türkiye

Güngör Yılmaz
0000-0003-0070-5484
Türkiye

Serkan Uranbey
0000-0002-0312-8099
Türkiye

Early Pub Date

June 10, 2025

Publication Date

July 4, 2025

Submission Date

December 5, 2024

Acceptance Date

April 17, 2025

Published in Issue

Year 2025 Volume: 28 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1596868

IZ

https://izlik.org/JA23AZ88KN

Cite

RIS / Bibtex

APA

Yaman, C., Yılmaz, G., & Uranbey, S. (2025). Influences of Light-Emitting Diodes (LEDs) and Culture Media on Micropropagation of Native Hemp (Cannabis sativa) Population from Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 1071-1082. https://doi.org/10.18016/ksutarimdoga.vi.1596868