Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria against Cown Rot Disease Agent Rhizoctonia solani AG 2-2 in Sugar Beet (Beta vulgaris L.)

Özden Salman; Nuh Boyraz

doi:10.18016/ksutarimdoga.vi.1611970

TR EN

Şeker Pancarında (Beta vulgaris L.) Taç Çürüklüğü Hastalık Etmeni Rhizoctonia solani AG 2-2’ye Karşı Kökbakterilerinin İzolasyonu, Tanılanması ve Biyokontrol Etkinliklerinin Belirlenmesi

Abstract

Bu çalışmanın amacı, şeker pancarı (Beta vulgaris L.) yumrularında sorun taç çürüklüğü hastalık etmeni Rhizoctonia solani AG 2-2'ye karşı bitki kök ve topraklardan izole edilmiş potansiyel antagonistik (BCAs) ve bitki gelişimini teşvik eden kökbakterilerinin (PGPR) hastalık etmenine karşı biyokontrol potansiyelini araştırmaktır. Potansiyel biyolojik kontrol etmeni bakteri izolatlarını elde etmek için 20 ilden toplam 219 toprak örneği toplanmıştır. Bu örneklerden 752 bakteri izolatı elde edilmiş ve bu bakterilerin, R. solani AG-2.2'ye karşı in vitro antifungal etkinlikleri, ikili kültür denemeleri ile test edilmiştir. Sonuçta, 117 bakteri izolatının misel gelişimini engelleyen antibiyosis özelliği gösterdiği belirlenmiştir. Bu izolatların antibiyosis etkinliklerinin yanısıra, fosfor çözünürlüğü, hidrojen siyanür (HCN) aktivitesi, siderofor üretimi, ACC deaminaz aktivitesi ve indol-3-asetik asit (IAA) üretimi gibi bitki gelişimini teşvik edici özellikleri karakterize edilmiştir. Test edilen PGPR izolatlar arasında, en iyi 12 bakteri, tartılı derecelendirme sistemi kullanılarak in vivo saksı testlerine tabi tutulmuştur. Bacillus subtilis 119.2 izolatı, şeker pancarında R. solani AG-2.2' kaynaklı hastalık çıkışının engellenmesinde %100 oranla en etkili izolat olarak belirlenmiştir. Hem in vitro hem de in vivo çalışma sonuçları göz önünde bulundurulduğunda, B. subtilis 119.2 izolatının şeker pancarı tarımında R. solani'ye karşı çevre dostu biyolojik mücadele etmeni olarak potansiyelinin bulunduğunu göstermiştir. Tüm sonuçlar birlikte değerlendirildiğinde B. subtilis 119.2 biyolojik gübre veya biyolojik fungisit olarak kullanım potansiyeline sahip PGPR izolatıdır.

Keywords

Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria against Cown Rot Disease Agent Rhizoctonia solani AG 2-2 in Sugar Beet (Beta vulgaris L.)

Abstract

This study aims to investigate the biocontrol potential of antagonistic and plant growth-promoting rhizobacteria (PGPR) isolated from plant roots and soils against the crown rot pathogen Rhizoctonia solani AG 2-2 in sugar beet (Beta vulgaris L.) tubers. A total of 219 soil samples were collected from 20 provinces to obtain putative bacterial PGPR isolates that could potentially be used as biological control agents (BCAs). A total of 752 bacterial isolates were obtained, and there in vitro antifungal activity against R. solani AG-2.2 was tested in double culture experiments. The result was that 117 PGPR bacterial isolates exhibited antibiotic properties that inhibited mycelial growth of the fungal pathogen. These isolates were also characterized for their antagonistic and plant growth-promoting properties, including phosphorus solubility, hydrogen cyanide (HCN) activity, siderophore production, ACC deaminase activity, and indole-3-acetic acid (IAA) production. The 12 best PGPR isolates were then subjected to an in vivo pot test using a weighted scoring system. The isolate Bacillus subtilis 119.2 was identified as the most effective isolate that completely inhibited the outbreak of the disease caused by R. solani AG-2.2 in sugar beet. Considering the results of the in vitro and in vivo studies, the B. subtilis 119.2 isolate showed its potential as an environmentally friendly biological control agent against R. solani in sugar beet. Overall, B. subtilis 119.2 is a PGPR isolate with potential for use as a biofertilizer or biofungicide.

Keywords

Supporting Institution

The project has been supported by the Selçuk University Faculty Member Placement Program with the project number 2019-ÖYP-011 for its execution.

Project Number

2019-ÖYP-011

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Details

Primary Language

English

Subjects

Plant Pathology, Phytopathology

Journal Section

Research Article

Authors

Özden Salman ^*
0000-0002-7871-4105
Türkiye

Nuh Boyraz
Türkiye

Early Pub Date

July 25, 2025

Publication Date

September 11, 2025

Submission Date

January 2, 2025

Acceptance Date

June 24, 2025

Published in Issue

Year 2025 Volume: 28 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1611970

IZ

https://izlik.org/JA22UA43AU

Cite

RIS / Bibtex

APA

Salman, Ö., & Boyraz, N. (2025). Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria against Cown Rot Disease Agent Rhizoctonia solani AG 2-2 in Sugar Beet (Beta vulgaris L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(5), 1241-1258. https://doi.org/10.18016/ksutarimdoga.vi.1611970

AMA

1.Salman Ö, Boyraz N. Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria against Cown Rot Disease Agent Rhizoctonia solani AG 2-2 in Sugar Beet (Beta vulgaris L.). KSU J. Agric Nat. 2025;28(5):1241-1258. doi:10.18016/ksutarimdoga.vi.1611970

Chicago

Salman, Özden, and Nuh Boyraz. 2025. “Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria Against Cown Rot Disease Agent Rhizoctonia Solani AG 2-2 in Sugar Beet (Beta Vulgaris L.)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 28 (5): 1241-58. https://doi.org/10.18016/ksutarimdoga.vi.1611970.

EndNote

Salman Ö, Boyraz N (September 1, 2025) Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria against Cown Rot Disease Agent Rhizoctonia solani AG 2-2 in Sugar Beet (Beta vulgaris L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28 5 1241–1258.

IEEE

[1]Ö. Salman and N. Boyraz, “Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria against Cown Rot Disease Agent Rhizoctonia solani AG 2-2 in Sugar Beet (Beta vulgaris L.)”, KSU J. Agric Nat., vol. 28, no. 5, pp. 1241–1258, Sept. 2025, doi: 10.18016/ksutarimdoga.vi.1611970.

ISNAD

Salman, Özden - Boyraz, Nuh. “Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria Against Cown Rot Disease Agent Rhizoctonia Solani AG 2-2 in Sugar Beet (Beta Vulgaris L.)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28/5 (September 1, 2025): 1241-1258. https://doi.org/10.18016/ksutarimdoga.vi.1611970.

JAMA

1.Salman Ö, Boyraz N. Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria against Cown Rot Disease Agent Rhizoctonia solani AG 2-2 in Sugar Beet (Beta vulgaris L.). KSU J. Agric Nat. 2025;28:1241–1258.

MLA

Salman, Özden, and Nuh Boyraz. “Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria Against Cown Rot Disease Agent Rhizoctonia Solani AG 2-2 in Sugar Beet (Beta Vulgaris L.)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 28, no. 5, Sept. 2025, pp. 1241-58, doi:10.18016/ksutarimdoga.vi.1611970.

Vancouver

1.Özden Salman, Nuh Boyraz. Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria against Cown Rot Disease Agent Rhizoctonia solani AG 2-2 in Sugar Beet (Beta vulgaris L.). KSU J. Agric Nat. 2025 Sep. 1;28(5):1241-58. doi:10.18016/ksutarimdoga.vi.1611970