Hypericum perforatum L. Kalluslarında Ekzojen Absisik Asidin Kolinesteraz Enzim Aktivitesi ve Fenolik Bileşik Değişkenliği Üzerine Etkinliği

Şeyma Önlü

doi:10.18016/ksutarimdoga.vi.1614168

EN TR

Efficacy of Exogenous Abscisic Acid on Cholinesterase Enzyme Activity and Phenolic Compound Variability in Hypericum perforatum calli

Abstract

The aim of this study was to investigate the effect of abscisic acid (ABA) on cholinesterase enzyme activities and bioactive components of in vitro calli of Hypericum perforatum. Calli were obtained from Linsmaier Skoog (LS)/B5 medium containing 0.5 mg L-1 thidiazuron (TDZ) and 0.5 mg L-1 indole butyric acid (IBA). Different abscisic acid (0.01 mg L-1, 0.05 mg L-1, and 0.1 mg L-1) applications were performed on the calli obtained in tissue culture and extracted in 80% methanol. Hispidulin, fumaric acid, acacetin, epicatechin, and naringenin compounds were analyzed in Liquid chromatography–high–resolution mass spectrometry (LC-HRMS). While epicatechin (1.09 mg L-1) was found at the highest 0.05 mg L-1 ABA in callus samples, fumaric acid (2.30 mg L-1) and hispidulin (0.78 mg L-1) compounds were detected in the highest containing of 0.1 mg L-1 ABA. Acacetin (0.10 mg L-1) was produced in only 0.01 mg L-1 ABA, whereas naringenin (0.01 mg L-1) was produced in medium containing 0.05 mg L-1 ABA. The strongest Acetylcholinesterase (AChE) (0.207±0.012) and Butyrylcholinesterase (BChE) (0.243±0.019) inhibition activity was shown the best with 0.1 mg L-1 ABA application. This study has shown that ABA can be used as an elicitor in callus culture of H. perforatum, and the amounts of related metabolites can be changed via elicitors

Keywords

Hypericum perforatum L. Kalluslarında Ekzojen Absisik Asidin Kolinesteraz Enzim Aktivitesi ve Fenolik Bileşik Değişkenliği Üzerine Etkinliği

Öz

Bu çalışmanın amacı, absisik asitin (ABA) Hypericum perforatum'un in vitro kalluslarının kolinesteraz enzim aktiviteleri ve biyoaktif bileşenleri üzerine etkisini araştırmaktır. Kalluslar, 0.5 mg L-1 thidiazuron (TDZ) ve 0.5 mg L-1 indol bütirik asit (IBA) içeren Linsmaier Skoog (LS)/B5 besiyerinden elde edildi. Doku kültüründe elde edilen kalluslara farklı absisik asit (0,01 mg L-1, 0,05 mg L-1 ve 0,1 mg L-1) uygulamaları yapıldı ve %80'lik metanolde ekstrakte edildi. Hispidulin, fumarik asit, akasetin, epikateşin, naringenin bileşikleri Sıvı Kromatografisi-Yüksek Çözünürlüklü Kütle Spektrometrisi (LC-HRMS) ile analiz edildi. Kallus örneklerinde en yüksek epikateşin (1.09 mg L-1) 0.05 mg L-1 ABA'da bulunurken, fumarik asit (2.30 mg L-1) ve hispidulin (0.78 mg L-1) bileşikleri en yüksek 0.1 mg L-1 ABA içerenlerde tespit edildi. Sadece 0.01 mg L-1 ABA içeren ortamda akasetin (0.10 mg L-1) üretilirken, 0.05 mg L-1 ABA içeren besin ortamında naringenin (0.01 mg L-1) üretildi. En güçlü Asetilkolinesteraz (AchE) (0.207±0.012) ve Bütirilkolinesteraz BChE (0.243±0.019) inhibisyon aktivitesinin 0.1 mg L -1 ABA uygulamasıyla olduğu ortaya çıktı. Bu çalışma, ABA'nın H.perforatum'un kallus kültüründe uyarıcı olarak kullanılabileceğini ve ilgili metabolitlerin miktarlarının elisitör aracılığıyla değiştirilebileceğini göstermiştir.

Anahtar Kelimeler

Supporting Institution

Muş Alparslan Üniversitesi, Bilimsel Araştırma proje birimi

Project Number

18-FEF-4901-02

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Details

Primary Language

Turkish

Subjects

Plant Biotechnology

Journal Section

Research Article

Authors

Şeyma Önlü ^*
0000-0003-2005-1019
Türkiye

Early Pub Date

June 10, 2025

Publication Date

July 4, 2025

Submission Date

January 6, 2025

Acceptance Date

May 17, 2025

Published in Issue

Year 2025 Volume: 28 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1614168

IZ

https://izlik.org/JA72HK84RL

Cite

RIS / Bibtex

APA

Önlü, Ş. (2025). Hypericum perforatum L. Kalluslarında Ekzojen Absisik Asidin Kolinesteraz Enzim Aktivitesi ve Fenolik Bileşik Değişkenliği Üzerine Etkinliği. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 1083-1094. https://doi.org/10.18016/ksutarimdoga.vi.1614168

Cited By

Integrated chemical and biological characterization of Hypericum perforatum extract using LC-MS/MS and in vitro functional assays

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https://doi.org/10.1038/s41598-026-36793-8