Ordu İli Yöresel Bitkilerinin (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum) İnsan Serviks Kanseri (HeLa) Hücre Hattına Karşı Antikanser Etkilerinin ve DNA Koruyucu Aktivitelerinin Araştırılması

Bülent Kaynak; Gülizar Aydoğdu; Zeynep Kolören; Onur Kolören

doi:10.18016/ksutarimdoga.vi.1651205

EN TR

Investigation of the Anticancer Effects on Human Cervical Cancer (HeLa) Cell Line and the DNA Protective Activities of Traditional Plants from Ordu Province (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum)

Abstract

This study investigates the protective potential of ethanolic extracts of local plants from Ordu province, Brassica oleracea var. viridis, Smilax excelsa, and Conium maculatum, against DNA damage and their anticancer activities in HeLa (cervix adenocarcinoma) cells. The extracts were prepared at varying concentrations, and their anticancer effects were evaluated using the 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) assay in HeLa cells. The ability of these extracts to prevent hydroxyl radical (-OH)-induced damage to pBR322 plasmid DNA was also examined. MTT assay results showed that cell viability decreased in a dose-dependent manner when treated with different concentrations of the extracts. The approximate IC50 values for S. excelsa, B. oleracea var. viridis, and C. maculatum extracts at 24 hours were determined to be 3.88, 2.20, and 1.48 mg/mL, respectively, while at 48 hours, these values were 1.82, 1.13, and 0.39 mg/mL. Furthermore, the extracts demonstrated strong protective activity at various concentrations (20, 10, and 5 mg/mL) in preventing OH-induced DNA damage. These findings provide preliminary data on the DNA protective and anticancer effects of ethanol extracts of S. excelsa, B. oleracea var. viridis, and C. maculatum, indicating that future studies should focus on identifying the active compounds responsible for these effects and investigating their therapeutic potential in more detail.

Keywords

Ordu İli Yöresel Bitkilerinin (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum) İnsan Serviks Kanseri (HeLa) Hücre Hattına Karşı Antikanser Etkilerinin ve DNA Koruyucu Aktivitelerinin Araştırılması

Öz

Bu çalışmada, Ordu iline ait yöresel bitkilerinden Brassica oleracea var. viridis, Smilax excelsa ve Conium maculatum’un etanolik özütlerinin DNA hasarına karşı koruyucu potansiyelleri ve insan serviks adenokarsinomu (HeLa) hücre hattına karşı antikanser aktiviteleri araştırılmıştır. Özütler, farklı konsantrasyonlarda hazırlanarak antikanser aktiviteleri, HeLa hücre hattında 3-(4,5-dimetiltiyazol-2-il)-2,5-difeniltetrazolyum bromid (MTT) testi ile değerlendirilmiştir. Özütlerin, hidroksil radikali (-OH) ile indüklenen pBR322 plazmid DNA hasarını engelleme potansiyelleri de araştırılmıştır. Özütlerin HeLa hücreleri ile yapılan MTT analizleri neticesinde, farklı konsantrasyonlarla yapılan uygulamalarda, hücre canlılığının doza bağlı olarak azaldığı gözlenmiştir. S. excelsa, B. oleracea var. viridis ve C. maculatum özütlerinin 24 saatteki yaklaşık IC50 değerleri sırasıyla 3.88, 2.20 ve 1.48 mg/mL, 48 saatteki yaklaşık IC50 değerleri ise sırasıyla 1.82, 1.13 ve 0.39 mg/mL olarak tespit edilmiştir. Ayrıca, -OH ile indüklenen DNA hasarını önleme açısından özütlerin farklı (20, 10 ve 5 mg/mL) konsantrasyonlarının güçlü koruyucu aktivite gösterdiği bulunmuştur. Bu bulgular, S. excelsa, B. oleracea var. viridis ve C. maculatum etanolik özütlerinin DNA koruyucu ve antikanser etkilerine dair öncü veriler sunmakta olup, ileri çalışmalarda bu etkilerden sorumlu aktif bileşiklerin belirlenmesi ve terapötik potansiyellerinin detaylı olarak incelenmesi gerektiğini ortaya koymaktadır.

Anahtar Kelimeler

Ethical Statement

Etik onay gerekmemektedir.

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Details

Primary Language

Turkish

Subjects

Plant Cell and Molecular Biology

Journal Section

Research Article

Authors

Bülent Kaynak ^*
0000-0002-9207-5485
Türkiye

Gülizar Aydoğdu
0000-0001-8390-1477
Türkiye

Zeynep Kolören
0000-0001-9708-2716
Türkiye

Onur Kolören
0000-0002-3359-4904
Türkiye

Early Pub Date

June 10, 2025

Publication Date

July 4, 2025

Submission Date

March 4, 2025

Acceptance Date

May 14, 2025

Published in Issue

Year 2025 Volume: 28 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1651205

IZ

https://izlik.org/JA95PJ46KG

Cite

RIS / Bibtex

APA

Kaynak, B., Aydoğdu, G., Kolören, Z., & Kolören, O. (2025). Ordu İli Yöresel Bitkilerinin (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum) İnsan Serviks Kanseri (HeLa) Hücre Hattına Karşı Antikanser Etkilerinin ve DNA Koruyucu Aktivitelerinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 945-954. https://doi.org/10.18016/ksutarimdoga.vi.1651205