Valorization of Strained Yogurt Wastewater as Production Medium Nutrient for Arthrospira platensis and Haematococcus pluvialis

Zülfiye Velioğlu Tosuner; Raziye Öztürk Ürek

doi:10.18016/ksutarimdoga.vi.1678318

TR EN

Süzme Yoğurt Altı Suyunun Arthrospira platensis ve Haematococcus pluvialis İçin Üretim Ortamı Besin Maddesi Olarak Değerlendirilmesi

Öz

Bu çalışma, süzme yoğurt atık suyunun (strained yogurt wastewater, SYW), Arthrospira platensis ve Haematococcus pluvialis mikroalglerinin miksotrofik ve heterotrofik koşullarda yetiştirilmesi için besin kaynağı olarak kullanımını araştırmaktadır. Süzme yoğurt üretiminin bir yan ürünü olan SYW, organik karbon ve protein açısından zengindir. A. platensis Zarrouk ortamında (pH 9,0; 30°C), H. pluvialis Basal Bold Medium (pH 7,0; 28°C) ortamında büyütülmüştür. Miksotrofik ve heterotrofik denemeler, büyüme ortamına farklı konsantrasyonlarda SYW (%1, 10 ve 30, v/v) eklenmesi ile gerçekleştirilmiştir. A. platensis, H. pluvialis’e kıyasla daha yüksek lipid, protein ve karbohidrat üretimi sergilemiştir. Miksotrofik koşullarda A. platensis, H. pluvialis'e göre 2 kat daha fazla lipid üretimi ile en yüksek verime ulaşmıştır. Bir sonraki aşamada, magnezyum, kalsiyum ve sodyum metallerinin bu iki mikroalg türünün lipid üretimine etkisi incelenmiştir. H. pluvialis, en yüksek lipid üretimini (2,68 mg lipid g-1 biyokütle) magnezyum içermeyen heterotrofik ortamda elde etmiştir. Buna karşılık, A. platensis en yüksek lipid üretimini (5,31 mg lipid g-1 biyokütle), büyüme ortamında magnezyumun normal konsantrasyonunun üç katına çıkarıldığı koşullarda göstermiştir. Her iki mikroalgden elde edilen lipidlerin yağ asidi profilleri belirlenmiş ve toplam yağ asidi içeriğinde yüksek oranda doymamış yağ asitleri bulunduğu ortaya konmuştur. Bu araştırma, mikroalg kültivasyonu için ilk defa kullanılmış olan SYW’nin sürdürülebilir bir besin kaynağı olma potansiyelini ortaya koymakta; mikroalg üretiminde ekonomik ve çevresel avantajlar sağlarken, aynı zamanda atık su arıtımı ve alg patlamalarının kontrolüne katkıda bulunmaktadır.

Anahtar Kelimeler

Valorization of Strained Yogurt Wastewater as Production Medium Nutrient for Arthrospira platensis and Haematococcus pluvialis

Abstract

The study investigates the utilization of strained yogurt wastewater (SYW) as a nutrient source for cultivating Arthrospira platensis and Haematococcus pluvialis in mixotrophic and heterotrophic conditions. SYW, a by-product of strained yogurt production, is rich in organic carbon and proteins. A. platensis was grown in Zarrouk medium (pH 9.0, 30°C) and H. pluvialis in Basal Bold Medium (pH 7.0, 28°C). Mixotrophic and heterotrophic experiments were carried out by adding different concentrations of SYW (1, 10 and 30%, v/v) to the growth medium. A. platensis exhibiting superior lipid, protein, and carbohydrate production compared to H. pluvialis. Optimal lipid production was achieved in A. platensis with a 2-fold increase over H. pluvialis in mixotrophic conditions. In the next stage, the effect of magnesium, calcium and sodium metals on the lipid production of these two microalgae was examined. H. pluvialis achieved its highest lipid (2.68 mg lipid g-1 biomass) production in a non-magnesium heterotrophic medium. In contrast, A. platensis's highest lipid production (5.31 mg lipid g-1 biomass) was achieved in a medium where magnesium was present at three times the concentration of the normal growth medium. The fatty acid profiles of lipids obtained by both microalgae have been determined, revealing a high proportion of unsaturation in the total fatty acid content. This research demonstrates the potential of SYW, which has been used for the first time for microalgae cultivation, as a sustainable food source; it provides economic and environmental advantages in microalgae production, while also contributing to wastewater treatment and algal bloom control.

Keywords

Ethical Statement

Gerekmemektedir.

References

Al-Kadamany, E., Toufeili, I., Khattar, M., Abou-Jawdeh, Y., Harakeh, S., & Haddad, T. (2002). Determination of shelf life of concentrated yogurt (Labneh) produced by in-bag straining of set yogurt using hazard analysis. Journal of Dairy Science, 85(5), 1023–1030. https://doi.org/10.3168/jds.S0022-0302(02)74162-3
Al-Shetwi, A. Q. (2022). Sustainable development of renewable energy integrated power sector: Trends, environmental impacts, and recent challenges. Science of the Total Environment, 822, 153645. http://dx.doi.org/10.1016/j.scitotenv.2022.153645
Anahas, A. M. P., Prasannabalaji, N., & Muralitharan, G. (2025). Enhancing biodiesel production in Anabaena sphaerica MBDU 105: exploring photo-, hetero-, and mixotrophic cultivation for biomass, lipid, and fuel properties. Biomass Conversion and Biorefinery, 15(5), 7927-7946. https://doi.org/10.1007/s13399-024-05640-z
Bhattacharya, M., & Goswami, S. (2020). Microalgae–A green multi-product biorefinery for future industrial prospects. Biocatalysis and Agricultural Biotechnology, 25:101580. https://doi.org/10.1016/j.bcab.2020.101580
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Buso, D., Zissis, G., & Prudhomme, T. (2021). Influence of light intensity and photoperiod on energy efficiency of biomass and pigment production of Spirulina (Arthrospira platensis). Oilseeds and Fats, Crops and Lipids, 28, 37-43. https://doi.org/10.1051/ocl/2021025
Cao, J., Yuan, H., Li, B., & Yang, J. (2014). Significance evaluation of the effects of environmental factors on the lipid accumulation of Chlorella minutissima UTEX 2341 under low-nutrition heterotrophic condition. Bioresource Technology, 152:177–184. https://doi.org/10.1016/j.biortech.2013.10.084
Carmen Muñoz-Marín, M., López-Lozano, A., Moreno-Cabezuelo, J. Á., Díez, J., & García-Fernández, J. M. (2024). Mixotrophy in cyanobacteria. Current Opinion in Microbiology, 78, 102432. https://doi.org/10.1016/j.mib.2024.102432

Cheirsilp, B., & Torpee, S. (2012). Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of organic carbon sources. Bioresource Technology, 110, 510–516. https://doi.org/10.1016/j.biortech.2012.01.125
Chisti, Y. (2013). Constraints to commercialization of algal fuels. Journal of Biotechnology, 167, 201–214. https://doi.org/10.1016/j.jbiotec.2013.07.020
Dahai, H., Zhihong, Y., Lin, Q., Yuhong, L., Lei, T., Jiang, L., & Liandong, Z. (2024). The application of magical microalgae in carbon sequestration and emission reduction: Removal mechanisms and potential analysis. Renewable and Sustainable Energy Reviews, 197, 114417. https://doi.org/10.1016/j.rser.2024.114417
De Morais, E. G., Nunes, I. L., Druzian, J. I., de Morais, M. G., da Rosa, A. P. C., & Costa, J. A. V. (2020). Increase in biomass productivity and protein content of Spirulina sp. LEB 18 (Arthrospira) cultivated with crude glycerol. Biomass Conversion and Biorefinery, 1-9. https://doi.org/10.1007/s13399-020-00934-4
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Biochemistry, 28(3), 350–356.
Elloumi, W., Jebali, A., Maalej, A., Chamkha, M., & Sayadi, S. (2020). Effect of mild salinity stress on the growth, fatty acid and carotenoid compositions, and biological activities of the thermal freshwater microalgae Scenedesmus sp. Biomolecules, 10(11), 1–17. https://doi.org/10.3390/biom10111515
Ermis, H., Guven-Gulhan, U., Cakir, T., & Altinbas, M. (2020). Effect of iron and magnesium addition on population dynamics and high value product of microalgae grown in anaerobic liquid digestate. Science Reports, 10(1), 3510. https://doi.org/10.1038/s41598-020-60622-1
Eryalçın, K. M. (2018). Farklı mikroalg ve ticari yemlerin rotifer (Brachionus plicatilis, Müller 1786) büyümesi, protein ve yağ asidi profiline etkisi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 21(2), 220-228. https://doi.org/10.18016/ksudobil.305572
Esen, M., & Ozturk Urek, R. (2015). Ammonium nitrate and iron nutrition effects on some nitrogen assimilation enzymes and metabolites in Spirulina platensis. Biotechnology and Applied Biochemistry, 62(2), 275–286. https://doi.org/10.1002/bab.1268
Feng, D., Wang, X., Gao, J., Zhang, C., Liu, H., Liu, P., & Sun, X. (2023). Exogenous calcium: Its mechanisms and research advances involved in plant stress tolerance. Frontiers in Plant Science, 14, 1143963. https://doi.org/10.3389/fpls.2023.1143963
Figueroa-Torres, G.M., Pittman, J.K., & Theodoropoulos, C. (2022). A highly productive mixotrophic fed-batch strategy for enhanced microalgal cultivation. Sustainable Energy & Fuels, 6(11), 2771–2782. https://doi.org/10.1039/D2SE00124A
Ganta, A., Bashir, Y., & Das, S. (2022). Dairy wastewater as a potential feedstock for valuable production with concurrent wastewater treatment through microbial electrochemical technologies. Energies, 15(23), 1–34. https://doi.org/10.3390/en15239084
Gao, P., Guo, L., Zhao, Y., Jin, C., She, Z., & Gao, M. (2021). Enhancing microalgae growth and product accumulation with carbon source regulation: New perspective for the coordination between photosynthesis and aerobic respiration. Chemosphere, 278, 130435. https://doi.org/10.1016/j.chemosphere.2021.130435
Ghanbarzadeh, M., Moazami, N., Mirdamadi, S., Shahavi, M. H., Moshaei, M. R., & Kiadeh, S. G. H. (2024). A study on some phytochemicals in Arthrospira platensis MGH-1 fortified with calcium and magnesium. Phycologia, 63(4), 390-398. https://doi.org/10.1080/00318884.2024.2351207
Gok, Z., Kucukcongar, S., Turkyilmaz, M., & Tutar Oksuz, S. (2023). Treatment of strained yoghurt wastewater by electrochemical oxidation method using Taguchi experimental design. Journal of Applied Electrochemistry, 53(8), 1595–1607. https://doi.org/10.1007/s10800-023-01866-3
Gorain, P. C., Bagchi, S. K., & Mallick, N. (2013). Effects of calcium, magnesium and sodium chloride in enhancing lipid accumulation in two green microalgae. Environmental Technology, 34(13-14), 1887-1894. https://doi.org/10.1080/09593330.2013.812668
Gupta, S., Marchetti, J. M., & Wasewar, K. L. (2024). Enhancing nutrient removal, biomass production, and biochemical production by optimizing microalgae cultivation in a mixture of untreated and anaerobically digested dairy wastewater. Journal of Water Process Engineering, 63, 105413. https://doi.org/10.1016/j.jwpe.2024.105413
Hasnain, M., Abideen, Z., Anthony Dias, D., Naz, S., & Munir, N. (2023). Utilization of saline water enhances lipid accumulation in green microalgae for the sustainable production of biodiesel. BioEnergy Research, 16(2), 1026–1039. https://doi.org/10.1007/s12155-022-10467-5
Indarti, E., Majid, M. I. A., Hashim, R., & Chong, A. (2005). Direct FAME synthesis for rapid total lipid analysis from fish oil and cod liver oil. Journal of Food Composition and Analysis, 18(2-3), 161–170. https://doi.org/10.1016/j.jfca.2003.12.007
Janet, R., Stein, E., & Stein, J. R. (Eds.). (1973). Handbook of Phycological Methods: Culture Methods and Growth Measurements. Cambridge: Cambridge University Press.
Kirdar, S. S., & Narman, K. (2021). Production parameters and some characteristics of Kökez strained yogurt. International Journal of Agriculture and Environmental Research, 07(03), 554–565. https://doi.org/10.51193/IJAER.2021.7313
Lee, S. Y., & Stuckey, D. C. (2022). Separation and biosynthesis of value-added compounds from food-processing wastewater: Towards sustainable wastewater resource recovery. Journal of Cleaner Production, 357, 131975. https://doi.org/10.1016/j.jclepro.2022.131975
Li, T., Yang, F., Xu, J., Wu, H., Mo, J., Dai, L., & Xiang, W. (2020). Evaluating differences in growth, photosynthetic efficiency, and transcriptome of Asterarcys sp. SCS-1881 under autotrophic, mixotrophic, and heterotrophic culturing conditions. Algal Research, 45, 101753. https://doi.org/10.1016/j.algal.2019.101753
Liang, Y., Sarkany, N., & Cui, Y. (2009). Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions. Biotechnology Letters, 31(7), 1043–1049. https://doi.org/10.1007/s10529-009-9975-7
Lichtenthaler, H. K., & Wellburn, A. R. (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 11, 591–592. https://doi.org/10.1042/bst0110591
Majhi, B. K. (2025). Cyanobacteria: Photosynthetic cell factories for biofuel production. Journal of Bioresources and Bioproducts, 10(2), 128-144. https://doi.org/10.1016/j.jobab.2024.10.001
Mishra, S. K., Suh, W. I., Farooq, W., Moon, M., Shrivastav, A., Park, M. S., & Yang, J. W. (2014). Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method. Bioresource Technology, 155, 330–333. https://doi.org/10.1016/j.biortech.2013.12.077
Mohapatra, J., Kumar, R., Basak, B., Saratale, R. G., Saratale, G. D., Mishra, A., Tripathy, S. J., Jeaon, B. H., & Chakrabortty, S. (2025). A review on generation, composition, and valorization of dairy processing sludge: A circular economy-based sustainable approach. Journal of Industrial and Engineering Chemistry, 143, 45-64. https://doi.org/10.1016/j.jiec.2024.08.045
Molotoks, A., Smith, P., & Dawson, T. P. (2021). Impacts of land use, population, and climate change on global food security. Food and Energy Security, 10(1), e261. https://doi.org/10.1002/fes3.261
Narayan, M. S., Manoj, G. P., Vatchravelu, K., Bhagyalakshmi, N., & Mahadevaswamy, M. (2005). Utilization of glycerol as carbon source on the growth, pigment and lipid production in Spirulina platensis. International Journal of Food Sciences and Nutrition, 56(7), 521–528.
Nikkanen, L., Solymosi, D., Jokel, M., & Allahverdiyeva, Y. (2021). Regulatory electron transport pathways of photosynthesis in cyanobacteria and microalgae: Recent advances and biotechnological prospects. Physiologia Plantarum, 173(2), 514–525. https://doi.org/10.1111/ppl.13404
Ozturk Urek, R., & Kerimoglu, Y. (2019). Evaluation of effects of Mg2+ and Cu2+ on pigment-metabolite production and photosystem II activity of Arthrospira platensis Gomont 1892. Turkish Journal of Fisheries and Aquatic Sciences, 19(10), 873–883. http://doi.org/10.4194/1303-2712-v19_10_07
Pires, A. F., Marnotes, N. G., Rubio, O. D., Garcia, A. C., & Pereira, C. D. (2021). Dairy by-products: A review on the valorization of whey and second cheese whey. Foods 10(5), 1067. https://doi.org/10.3390/foods10051067
Polat, E., Yüksel, E., & Altınbaş, M. (2020). Mutual effect of sodium and magnesium on the cultivation of microalgae Auxenochlorella protothecoides. Biomass Bioenergy 132, 105441. https://doi.org/10.1016/j.biombioe.2019.105441
Qiao, T., Zhao, Y, Zhong, D. B., & Yu, X. (2021). Hydrogen peroxide and salinity stress act synergistically to enhance lipids production in microalga by regulating reactive oxygen species and calcium. Algal Research, 53, 102017 https://doi.org/10.1016/j.algal.2020.102017
Russo, N. P., Ballotta, M., Usai, L., Torre, S., Giordano, M., Fais, G., Casula, M., Dessi, D., Nieri, P., Damergi, E., Lutzu, G. A., & Concas, A. (2024). Mixotrophic cultivation of Arthrospira platensis (Spirulina) under salt stress: effect on biomass composition, FAME profile and phycocyanin content. Marine Drugs, 22(9), 381. https://doi.org/10.1007/s13399-024-05640-z
Salman, J. M., Grmasha, R. A., Stenger-Kovács, C., Lengyel, E., Al-Sareji, O. J., Al-Cheban, A. M. A., & Meiczinger, M. (2023). Influence of magnesium concentrations on the biomass and biochemical variations in the freshwater algae, Chlorella vulgaris. Heliyon, 9(1), e13072. https://doi.org/10.1016/j.heliyon.2023.e13072
Shahid, A., Khan, F., Ahmad, N., Farooq, M., & Mehmood, M. A. (2020). Microalgal carbohydrates and proteins: Synthesis, extraction, applications, and challenges. Microalgae Biotechnology Food Health Products, 43-468. https://doi.org/10.1007/978-981-15-0169-2_14
Silva, T. L. D., Moniz, P., Silva, C., & Reis, A. (2021). The role of heterotrophic microalgae in waste conversion to biofuels and bioproducts. Processes, 9(7), 1090. https://doi.org/10.3390/pr9071090
Sivakaminathan, S., Wolf, J., Yarnold, J., Roles, J., Ross, I. L., Stephens, E., Henderson, G., & Hankamer, B. (2020). Light guide systems enhance microalgae production efficiency in outdoor high rate ponds. Algal Research, 47, 101846. https://doi.org/10.1016/j.algal.2020.101846
Srinivasan, G. R., Shankar, V., Chandra Sekharan, S., Munir, M., Balakrishnan, D., Mohanam, A., & Jambulingam, R. (2020). Influence of fatty acid composition on process optimization and characteristics assessment of biodiesel produced from waste animal fat. Energy Sources Part A: Recovery, Utilization, and Environmental Effects, 46(1), 8842-8860. https://doi.org/10.1080/15567036.2020.1771477
Song, X., Liu, B. F., Kong, F., Ren, N. Q., & Ren, H. Y. (2022). Overview on stress-induced strategies for enhanced microalgae lipid production: Application, mechanisms and challenges. Resources, Conservation and Recycling. https://doi.org/10.1016/j.resconrec.2022.106355
Teng, Z., Zheng, L., Yang, Z., Li, L., Zhang, Q., Li, L., Chen, W., Wang, G., & Song, L. (2023). Biomass production and astaxanthin accumulation of Haematococcus pluvialis in large-scale outdoor culture based on year-round survey: Influencing factors and physiological response. Algal Research, 71, 103070. https://doi.org/10.1016/j.algal.2023.103070
Udayan, A., Pandey, A. K., Sirohi, R., Sreekumar, N., Sang, B. I., Sim, S. J., Kim, S. H., & Pandey, A. (2023). Production of microalgae with high lipid content and their potential as sources of nutraceuticals. Phytochemistry Reviews, 22(4), 833–860. https://doi.org/10.1007/s11101-021-09784-y
Udourioh, G. A., Solomon, M. M., & Okolie, J. A. (2025). A Review of the valorization of dairy industry wastes through thermochemical, biological, and integrated processes for value-added products. Food Science of Animal Resources, 45(2), 375. https://doi.org/10.5851/kosfa.2025.e2
Velioglu Tosuner, Z., & Ozturk Urek, R. (2021a). Simultaneous lipid production and valorization of crude glycerol by mixotrophic and heterotrophic cultivation of Arthrospira platensis. Journal of Scientific & Industrial Research, 80(10), 850–857.
Velioglu Tosuner, Z., & Ozturk Urek, R. (2021b). Effects of nutrients on lipids and biodiesel production potential of Haematococcus pluvialis. Environmental Engineering and Management Journal, 20(8),1289–1299.
Vonshak, A. (ed) (1997). Spirulina platensis arthrospira: Physiology, cell-biology and biotechnology (1st ed.). CRC Press, New York.
Wajs, J., Brodziak, A., & Król, J. (2023). Shaping the physicochemical, functional, microbiological and sensory properties of yoghurts using plant additives. Foods, 12(6), 2–30. https://doi.org/10.3390/foods12061275
Wang, Y., Chiu, S. Y., Ho, S. H., Liu, Z., Hasunuma, T., Chang, T. T., Chang K. F., Chang J. S., Ren N. Q., & Kondo, A. (2016). Improving carbohydrate production of Chlorella sorokiniana NIES‐2168 through semi‐continuous process coupled with mixotrophic cultivation. Biotechnology Journal, 11(8), 1072-1081. https://doi.org/10.1002/biot.201500270
Yaakob, M. A., Mohame, R. M. S. R., Al-Gheethi, A., Aswathnarayana Gokare, R., & Ambati, R. R. (2021). Influence of nitrogen and phosphorus on microalgal growth, biomass, lipid, and fatty acid production: an overview. Cells, 10(2), 393. https://doi.org/10.3390/cells10020393
Yaygın, H. (1999). Yoğurt Teknolojisi. Akdeniz Üniversitesi Yayını. 75, 331–334.
Zarrouk, C. (1966). Contribution à l’étude d’une cyanophycée. Influence de divers facteurs physiques et chimiques sur la croissance et la photosynthèse de Spirulina maxima. PhD Thesis, Université de Paris.
Zibarev, N., Toumi, A., Politaeva, N., & Iljin, I. (2024). Nutrients recovery from dairy wastewater by Chlorella vulgaris and comparison of the lipid’s composition with various Chlorella strains for biodiesel production. Plos one, 19(4), e0297464. https://doi.org/10.1371/journal.pone.0297464

Details

Primary Language

English

Subjects

Microbial Ecology , Aquaculture

Journal Section

Research Article

Authors

Zülfiye Velioğlu Tosuner
0000-0001-9181-6619
Türkiye

Raziye Öztürk Ürek ^*
0000-0002-7147-6853
Türkiye

Early Pub Date

April 11, 2026

Publication Date

-

Submission Date

April 17, 2025

Acceptance Date

December 22, 2025

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1678318

IZ

https://izlik.org/JA37SD94GW

Cite

RIS / Bibtex

APA

Velioğlu Tosuner, Z., & Öztürk Ürek, R. (2026). Valorization of Strained Yogurt Wastewater as Production Medium Nutrient for Arthrospira platensis and Haematococcus pluvialis. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication, 1353-1368. https://doi.org/10.18016/ksutarimdoga.vi.1678318