Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena leucocephala Silage

Mert Özmen; Kadir Emre Buğdaycı

doi:10.18016/ksutarimdoga.vi.1698057

TR EN

Farklı Katkı Maddelerinin Leucaena leucocephala Silajının Bazı Kimyasal Parametreleri ve In Vitro Sindirilebilirliği Üzerine Etkileri

Abstract

Bu çalışma, çeşitli katkı maddelerinin Leucaena leucocephala silajının fiziksel ve kimyasal özellikleri, yem değeri ve in vitro sindirilebilirliği üzerindeki etkilerini araştırmıştır. Araştırma, bir kontrol grubu ve sırasıyla %5 melas, %5 arpa ve %0.5 formik asit ile ilave edilen üç deneme grubundan oluşmuştur. Her grup sekiz tekerrürden oluşturulmuştur. Bitki materyali 2,5-5 cm boyutunda kesilmiş, ilgili katkı maddeleri ile işlenmiş ve vakumlanarak paketlenmiştir (200 g torba). 60 günlük silaj fermentasyon süresinin ardından, fiziksel özellikler, amonyak azotu, pH seviyeleri, besin kompozisyonu, yem değeri, in vitro ham protein parçalanabilirliği (IVHPP) ve in vitro sindirilebilirliği değerlendirmek için analizler yapılmıştır. DLG puanlarına göre (≥17.62±0.29), tüm gruplar yüksek kaliteli silaj sınıfında yer almaktadır. Uygulamalar yapısal olmayan besin maddelerini önemli düzeyde etkilemese de, %5 arpa grubunun ham kül içeriğinde önemli düzeyde bir artış gözlemlenmiştir. Leucaena silajına %5 melas ilavesi, ham selüloz (p<.005) ve asit deterjan fiber (p≤.001) düzeylerini önemli düzeyde azaltmış ve Flieg skorunu artırmıştır. Kuru madde tüketimi (KMT) dışında incelenen tüm yem değeri parametreleri %5 melas ilavesi ile önemli düzeyde artmıştır (p≤.001). Leucaena silajının metabolize olabilir enerjisinde önemli bir değişiklik gözlemlenmemiştir. %5 melas ilavesi, in vitro gerçek kuru madde sindirilebilirliği (IVGKMS) ve in vitro gerçek organik madde sindirilebilirliğini (IVGOMS) önemli düzeyde arttırmıştır (p≤.001), IVHPP'yi etkilememiştir. Sonuç olarak, bu çalışmada değerlendirilen silaj katkı maddeleri silaj kalitesini olumsuz etkilememiş ve %5 melas ilavesi en olumlu silaj kalite parametrelerini vermiştir.

Keywords

Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena leucocephala Silage

Abstract

This study investigated the effects of various additives on the physical and chemical properties, feed value, and in vitro digestibility of Leucaena leucocephala silage. The experiment consisted of one control group and three treatment groups, which were supplemented with 5% molasses, 5% barley, and 0.5% formic acid, respectively. Each group included eight replicates. The plant material was chopped (2.5–5 cm), treated with the respective additives, and vacuum-packed (200 g per bag). Following a 60-day silage fermentation period, analyses were conducted to evaluate physical properties, ammonia nitrogen, pH levels, nutrient composition, feed value, in vitro crude protein degradability (IVCPD), and in vitro digestibility. According to DLG scores (≥17.62±0.29), all groups produced high-quality silages. While treatments did not significantly affect non-structural nutrients, a notable increase in crude ash content was observed in the 5% barley group. The addition of 5% molasses to Leucaena silage significantly reduced crude fiber (p<.005) and acid detergent fiber (p≤.001) levels, while also increasing the Flieg score. All feed value parameters except dry matter intake (DMI) increased significantly with the addition of 5% molasses (p≤001). No significant changes were observed in the metabolisable energy of Leucaena silage. The addition of 5% molasses significantly increased in vitro true dry matter digestibility (IVTDMD) and in vitro true organic matter digestibility (IVTOMD) (p≤.001), while it did not affect IVCPD. In conclusion, the silage additives evaluated in this study did not negatively affect silage quality, and the inclusion of 5% molasses yielded the most favorable silage quality parameters.

Keywords

Supporting Institution

This research was supported by the Burdur Mehmet Akif Ersoy University Scientific Research Projects Commission (Project Number: 0965-YL-23).

Project Number

0965-YL-23

Ethical Statement

The project titled "Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena leucocephala Silage" was approved by the Local Ethics Committee for Animal Experiments of Burdur Mehmet Akif Ersoy University with the decision dated 23.08.2023 and numbered 1160. The board unanimously decided that there was no need for an ethics committee decision since the study would not be conducted on live animals.

Thanks

The researchers would like to thank Burdur Mehmet Akif Ersoy University.

References

Ahsan, U. (2023). Effect of single or combined homo-and heterofermentative silage additives on the quality, nutritive value, and ın vitro digestibility of ensiled wheat harvested at early dough stage of maturity. Mehmet Akif Ersoy University Health Sciences Institute,11(2), 267-274. https://doi.org/10.24998/maeusabed.1345229
Andrade, W. R., Moura, M. M. A., Rocha, V. R., Costa, R. F., Santos, L. H. T., & Silva, M. M. (2019). Quality of sorghum silage with leucaena. Acta Scientiarum Animal Sciences, 41(1), e36493. https://doi.org/10.4025/actascianimsci.v41i1.36493
AOAC. (2019). Official methods of analysis (21st ed.). Association of Official Analytical Chemists International. Araújo, J. S., Araújo, C. A., Macedo, Ad., Silva, C. S., Novaes, J. J. S., Lima, D. O., Borges, E. N., Gois, G. C., Araújo, G.
G. L., & Campos, F. S. (2022). Fermentation dynamics, nutritional quality, and heating capacity of mixed silages of elephant grass (Pennisetum purpureum Schum) and Leucaena (Leucaena leucocephala). Brazilian Journal of Veterinary Research and Animal Science, 59, e189466. https://doi.org/10.11606/issn.1678-4456.bjvras.2022.189466
Arslan, M., Erdurmuş, C., Öten, M., Aydınoğlu, B., & Çakmakçı, S. (2017). Mısır (zea mays l.) ile Leucaena leucocephala l. bitkisinin karıştırılmasıyla hazırlanan silajların besin değerinin belirlenmesi. Ege Üniversitesi Ziraat Fakültesi Dergisi, 54 (1), 101-106.
Avcıoğlu, R., Hatipoğlu, R., & Karadağ, Y. (2009). Yembitkileri: Baklagil yem bitkileri. T.C. Tarım ve Köy İşleri Bakanlığı Yayınları.
Aykan, Y., & Saruhan, V. (2018). Farklı oranlarda silolanan yembezelyesi (pisum sativum l.) ve arpa (hordeum vulgare l.) karışımlarının silaj kalite özelliklerinin belirlenmesi. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 11(2), 64-70.
Barros-Rodríguez, M., Solorio-Sánchez, J., Sandoval-Castro, C., Klieve, A. V., Briceño-Poot, E., Ramírez-Avilés, L., & Rojas-Herrera, R. (2013). Effects of two intake levels of Leucaena leucocephala on rumen function of sheep. Tropical Grasslands – Forrajes Tropicales, 1(1), 55–57. https://doi.org/10.17138/tgft(1)55 57

Barry, T. N., & Duncan, S. J. (1984). The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep. British Journal of Nutrition, 51(3), 485–491. https://doi.org/10.1079/bjn19840054
Buğdaycı, K. E., Karakaş Oğuz, F., Kara, K., Oğuz, M. N., Gülle, İ., Kocasarı, F., & Kocaoğlu Güçlü, B. (2025). Influence of harvesting periods and additives on the quality of silage from common reed (Phragmites australis) and cattail (Typha spp.). Journal of Animal and Feed Sciences, 34(2), 249–258. https://doi.org/10.22358/jafs/193488/2024
Buğdaycı, K. E., Oğuz, M. N., Canbay, H. S., Gümüş, H., & Oğuz, F. K. (2023). Using rose pulp silage instead of sugar beet pulp silage in lambs fed with total mixed ration. Turkish Journal of Veterinary and Animal Sciences, 47, 42-54. https://doi.org/10.55730/1300-0128.4267
Castro-González, A., Montero-Lagunes, M., Ríos-Utrera, Á., Ayala-Burgos, A. J., & Juárez-Lagunes, F. I. (2022). Leucaena leucocephala (Lam.) de Wit as protein supply for heifers. Agro Productividad, 15(7), 69–77. https://doi.org/10.32854/agrop.v15i7.2324
Crampton, E. W., & Maynard, L. (1938). The relation of cellulose and lignin content to nutritive value of animal feeds. The Journal of Nutrition, 15, 383-395. https://doi.org/10.1093/jn/15.4.383
Çakmakçı, S., Aydın, B., Çatlıoğlu, Ş. G., Köseoğlu, A. T. & Kaya, H. (2001). Farklı toprak koşullarının Leucaena leucocephala'nın çimlenme karakteristikleri üzerine etkileri. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi , 14(1), 161 - 170.
Dawson, B., & Trapp, R. G. (2001). Basic and clinical biostatistics (3th ed.). McGraw-Hill Medical Publishing Division.
DLG (1987). Bewertung von grünfutter, silage und heu (Merkblatt No.224). DLGVerlag, Deutschland.
Dong, Z., Wang, S., Zhao, J., & Li, J. (2020). Effects of additives on the fermentation quality, in vitro digestibility and aerobic stability of mulberry (morus alba l.) leaves silage. Asian-Australasian Journal of Animal Sciences, 33(8), 1292-1300. https://doi.org/10.5713/ajas.19.0420
Ferreira, R., Bezerra, L., Edvan, R., Araujo, M., Marques, C., Torreao, J., Oliveira, R., & Parente, H. (2016). Physicochemical composition and ruminal degradability of leucaena ensiled with different levels of buriti fruit peel. Grassland Science, 62, 160–166. https://doi.org/10.1111/grs.12129
Giang, N. T. T., Wanapat, M., Phesatcha, K., & Kang, S. (2016). Level of Leucaena leucocephala silage feeding on intake, rumen fermentation, and nutrient digestibility in dairy steers. Tropical Animal Health and Production, 48, 1057–1064. https://doi.org/10.1007/s11250-016-1060-3
Goering, J. K., & Van Soest, P. J. (1970). Forage Fiber Analysis. USDA-ARS Agricultural Handbook 379.
Gül, Z. D., Tan, M., Kaynar, D. F., & Kharazmi, K. (2015). Effects of some additives, harvest stage and wilting on quality characteristics of alfalfa silage. Atatürk University Journal of Agricultural Faculty, 46 (2), 113-118.
Güngör, T., Başalan, M., & Aydoğan, İ. (2008). Kırıkkale yöresinde üretilen bazı kaba yemlerde besin madde miktarları ve metabolize olabilir enerji düzeylerinin belirlenmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 55, 111-115. https://doi.org/10.1501/Vetfak_0000000299
Habermann, E., Oliveira, E. A. D., Contin, D. R., Costa, J. V. C. P., Costa, K. A. P., & Martinez, C. A. (2022). Warming offsets the benefits of elevated CO2 in water relations while amplifies elevated CO2-induced reduction in forage nutritional value in the C4 grass megathyrsus maximus. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.1033953
Karadeniz, E., & Saruhan, V. (2021). Mardin ekolojik koşullarında farklı ekim zamanlarında yetiştirilen ikinci ürün silajlık mısır (Zea mays L.) çeşitlerinin silaj özelliklerinin araştırılması. ISPEC Journal of Agricultural Sciences, 5(2), 275-289. https://doi.org/10.46291/ISPECJASvol5iss2pp275-289
Khaing, M., Aung, M., Htun, M. T., Mu, K. S., Aung, A., & Ngwe, T. (2016). Effect of leucaena forage and silage substitution in concentrates on digestibility, nitrogen utilization and milk yield in dairy cows. Journal of Applied and Advanced Research,1(3), 37-43. https://doi.org/10.21839/jaar.2016.v1i3.32
Kjeldahl, J. (1883). A new method for the determination of nitrogen in organic matter. Zeitschrift für Analytische Chemie, 22, 366-382.
Mohamed, M. I., El-Kady, R. I., El-Shahat, A. A., El-Banna, H. M., & Mohamed, S. G. (2000). Utilization of fresh Leucaena leucocephala plant and its silage in sheep nutrition. Journal of Animal and Poultry Production, 25(10), 6085-6094. https://doi.org/10.21608/jappmu.2000.259804
Moselhy, M. A., Borba, J. P., & Borba, A. E. S. (2015). Improving the nutritive value, in vitro digestibility and aerobic stability of hedychium gardnerianum silage through application of additives at ensiling time. Animal Feed Science and Technology, 206, 8–18. https://doi.org/10.1016/j.anifeedsci.2015.05.001
Phesatcha, K., & Wanapat, M. (2016). Improvement of nutritive value and ın vitro ruminal fermentation of Leucaena silage by molasses and urea supplementation. Asian-Australasian Journal of Animal Sciences, 29(8), 1136–1144. https://doi.org/10.5713/ajas.15.0591
Putra, R. A., Noviandi, C. T., & Umami, N. (2017). Digestibility and ruminal fermentation characteristic of native grass silage supplementation with different levels of Leucaena leucocephala. The 7th International Seminar on Tropical Animal Production on Food Sovereignty in Tropical Countries, Yogyakarta, Indonesia. https://journal.ugm.ac.id/istapproceeding/issue/archive
Santana, P. A., Cisneros, L. M., Martinez, A. Y., & Pascual, S. Y. (2015). Conservation and chemical composition of Leucaena leucocephala plus fresh or wilted pennisetum purpureum mixed silages. Revista MVZ Córdoba, 20, 4895-4906. https://doi.org/10.21897/rmvz.5
Seidavi, A., Tavakoli, M., Rasouli, B., Corazzin, M., & Salem, A. Z. M. (2020). Application of some trees/shrubs in ruminant feeding: a review. Agroforestry Systems, 94, 1353–1364. https://doi.org/10.1007/s10457-018-0313-8
SPSS (2013). IBM SPSS Statistics 21.0 for Windows. Armonk, NY.
Sutaryono, Y., Putra, R., Mardiansyah, M., Yuliani, E., Harjono, H., Mastur, M., Sukarne, S., Enawati, L. S., & Dahlanuddin, D. (2023). Mixed Leucaena and molasses can increase the nutritional quality and rumen degradation of corn stover silage. Journal of Advanced Veterinary and Animal Research, 10(1), 118. https://doi.org/10.5455/javar.2023.j660
Ülger, A. C., Tükel, T., & Hatipoğlu, R. (1990). Çukurova koşullarında leucaena leucocephala bitkisinden sonra yetiştirilen mısırda farklı azot miktarlarının tane verimi ve verim ögelerine etkisi.. Çukurova Üniversitesi Ziraat Fakültesi Dergisi, 7(3), 161-172.
Voltolini, T., Belem, K., Araújo, G., Moraes, S., Góis, G., & Campos, F. (2019). Quality of Leucaena, gliricidia, and pornunça silages with different old man saltbush levels. Semina-Ciencias Agrarias, 40(1), 2363-2374. https://doi.org/10.5433/1679-0359.2019v40n5Supl1p2363
Zapata-Campos, C. C., García-Martínez, J. E., Salinas-Chavira, J., Ascacio-Valdés, J. A., Medina-Morales, M. A., & Mellado, M. (2020). Chemical composition and nutritional value of leaves and pods of Leucaena leucocephala, prosopis laevigata and acacia farnesiana in a xerophilous shrubland. Emirates Journal of Food and Agriculture, 32(10), 723-730. https://doi.org/10.9755/ejfa.2020.v32.i10.2148
Zhang, Y., Li, M., Zhou, H., Hu, L., Li, W., & Xu, T. (2018). Associative effects of stylo and king grass silage different ratios on in vitro rumen fermentation. Legume Research, 41(4), 584-588. https://doi.org/10.18805/LR-335

Details

Primary Language

English

Subjects

Animal Feeding

Journal Section

Research Article

Authors

Mert Özmen
0009-0001-0685-5005
Türkiye

Kadir Emre Buğdaycı ^*
0000-0002-1715-6904
Türkiye

Early Pub Date

August 14, 2025

Publication Date

October 20, 2025

Submission Date

May 12, 2025

Acceptance Date

July 10, 2025

Published in Issue

Year 2025 Volume: 28 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1698057

IZ

https://izlik.org/JA34FM64DM

Cite

RIS / Bibtex

APA

Özmen, M., & Buğdaycı, K. E. (2025). Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena leucocephala Silage. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(6), 1616-1626. https://doi.org/10.18016/ksutarimdoga.vi.1698057

AMA

1.Özmen M, Buğdaycı KE. Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena leucocephala Silage. KSU J. Agric Nat. 2025;28(6):1616-1626. doi:10.18016/ksutarimdoga.vi.1698057

Chicago

Özmen, Mert, and Kadir Emre Buğdaycı. 2025. “Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena Leucocephala Silage”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 28 (6): 1616-26. https://doi.org/10.18016/ksutarimdoga.vi.1698057.

EndNote

Özmen M, Buğdaycı KE (October 1, 2025) Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena leucocephala Silage. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28 6 1616–1626.

IEEE

[1]M. Özmen and K. E. Buğdaycı, “Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena leucocephala Silage”, KSU J. Agric Nat., vol. 28, no. 6, pp. 1616–1626, Oct. 2025, doi: 10.18016/ksutarimdoga.vi.1698057.

ISNAD

Özmen, Mert - Buğdaycı, Kadir Emre. “Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena Leucocephala Silage”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28/6 (October 1, 2025): 1616-1626. https://doi.org/10.18016/ksutarimdoga.vi.1698057.

JAMA

1.Özmen M, Buğdaycı KE. Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena leucocephala Silage. KSU J. Agric Nat. 2025;28:1616–1626.

MLA

Özmen, Mert, and Kadir Emre Buğdaycı. “Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena Leucocephala Silage”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 28, no. 6, Oct. 2025, pp. 1616-2, doi:10.18016/ksutarimdoga.vi.1698057.

Vancouver

1.Mert Özmen, Kadir Emre Buğdaycı. Effects of Different Additives on Some Chemical Parameters and In Vitro Digestibility of Leucaena leucocephala Silage. KSU J. Agric Nat. 2025 Oct. 1;28(6):1616-2. doi:10.18016/ksutarimdoga.vi.1698057