Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]

Büşra Küçükkılııç; Öner Canavar; Hatice Kübra Gören

doi:10.18016/ksutarimdoga.vi.1699900

TR EN

Tuz Stresinin Fizyolojik Özellikler, Malondialdehit ve Prolin Birikimi Üzerindeki Etkileri ve Soya Fasulyesi [Glycine Max (L) Merrill]'Nin Ozmotik Ayarlamasi Ile İlişkisi

Abstract

Bu çalışma, on adet soya (Glycine max L. Merrill) çeşidinin fide döneminden V4 (üçüncü üç yaprak düğümü) büyüme evresine kadar, üç farklı tuz konsantrasyonuna (0, 3 ve 6 dS m⁻¹) karşı morfolojik, fizyolojik ve biyokimyasal tepkilerini değerlendirmiştir. Artan tuz düzeyleri; bitki boyu, yaş ve kuru ağırlık, yaprak alanı, göreceli su içeriği, klorofil a, klorofil b, SPAD ve karotenoid miktarlarında anlamlı azalmaya yol açarken; göreceli membran geçirgenliği (RMP), prolin ve malondialdehit (MDA) düzeylerinde artışa neden olmuştur. Bu çalışma, tuz stresi ile göreceli membran geçirgenliği (RMP) arasında 0.821 düzeyinde pozitif bir korelasyon olduğunu ortaya koymuştur. Ayrıca, tüm soya çeşitlerinde artan yaprak geçirgenliği, oksidatif zarara bağlı olarak diğer ölçülen özelliklerle anlamlı düzeyde negatif korelasyonlar göstermiştir.Özellikle yüksek MDA ve düşük prolin içeriğine sahip çeşitlerin tuz stresine karşı daha az kuru madde ürettiği görülmüş; bu da oksidatif zararın ve sınırlı osmotik koruyucu birikiminin etkisini ortaya koymuştur. Bulgular, prolin birikiminin tuza dayanıklı genotiplerin belirlenmesinde ve tuzlu koşullarda ürün dayanımının artırılmasında önemli bir gösterge olduğunu göstermektedir. Ayrıca, prolin ve MDA düzeylerinin çeşitler arasında farklılık göstermesi, tuz stresine karşı genotip özgü savunma mekanizmalarının varlığını ortaya koymuştur.

Keywords

Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]

Abstract

This study evaluated the morphological, physiological, and biochemical responses of ten soybean (Glycine max L. Merrill) cultivars to three levels of salt stress (0, 3, and 6 dS m⁻¹) from seedling emergence to the V4 growth stage. Increasing salt concentrations significantly reduced plant height, fresh and dry weights, leaf area, relative water content, chlorophyll a, chlorophyll b, SPAD, and carotenoid levels. In contrast, relative membrane permeability (RMP), proline, and malondialdehyde (MDA) levels increased with salt stress. This study showed that there was a 0.821** positive correlation between salt stress and rmp with the increasing salt stress, besides the increasing leaf permeability significantly in the leaves of all cultivars had a negatively significant correlation relationship with other measured traits, because of oxidative membrane damage. Notably, cultivars with higher MDA and lower proline contents produced less biomass under stress, emphasizing the role of oxidative damage and limited osmoprotectant accumulation. These findings underline proline accumulation as a key indicator for selecting salt-tolerant genotypes and improving crop resilience in saline environments. Additionally, the variation in proline and MDA responses among cultivars suggests genotype-specific mechanisms of salt stress tolerance.

Keywords

Project Number

ZRF-21047

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Details

Primary Language

English

Subjects

Agronomy

Journal Section

Research Article

Authors

Büşra Küçükkılııç
0000-0002-1479-7909
Türkiye

Öner Canavar
0000-0003-4168-953X
Türkiye

Hatice Kübra Gören ^*
0000-0001-7654-1450
Türkiye

Early Pub Date

October 18, 2025

Publication Date

January 1, 2026

Submission Date

May 15, 2025

Acceptance Date

August 15, 2025

Published in Issue

Year 2026 Volume: 29 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1699900

IZ

https://izlik.org/JA54MG84WN

Cite

RIS / Bibtex

APA

Küçükkılııç, B., Canavar, Ö., & Gören, H. K. (2026). Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 29(1), 79-97. https://doi.org/10.18016/ksutarimdoga.vi.1699900

AMA

1.Küçükkılııç B, Canavar Ö, Gören HK. Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]. KSU J. Agric Nat. 2026;29(1):79-97. doi:10.18016/ksutarimdoga.vi.1699900

Chicago

Küçükkılııç, Büşra, Öner Canavar, and Hatice Kübra Gören. 2026. “Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 29 (1): 79-97. https://doi.org/10.18016/ksutarimdoga.vi.1699900.

EndNote

Küçükkılııç B, Canavar Ö, Gören HK (January 1, 2026) Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 29 1 79–97.

IEEE

[1]B. Küçükkılııç, Ö. Canavar, and H. K. Gören, “Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]”, KSU J. Agric Nat., vol. 29, no. 1, pp. 79–97, Jan. 2026, doi: 10.18016/ksutarimdoga.vi.1699900.

ISNAD

Küçükkılııç, Büşra - Canavar, Öner - Gören, Hatice Kübra. “Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 29/1 (January 1, 2026): 79-97. https://doi.org/10.18016/ksutarimdoga.vi.1699900.

JAMA

1.Küçükkılııç B, Canavar Ö, Gören HK. Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]. KSU J. Agric Nat. 2026;29:79–97.

MLA

Küçükkılııç, Büşra, et al. “Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 29, no. 1, Jan. 2026, pp. 79-97, doi:10.18016/ksutarimdoga.vi.1699900.

Vancouver

1.Büşra Küçükkılııç, Öner Canavar, Hatice Kübra Gören. Salt Stress Effects Physiology Properties, Malondialdehyde and Proline Accumulation in Relation To Osmotic Adjustment Of Soybean [Glycine Max (L) Merrill]. KSU J. Agric Nat. 2026 Jan. 1;29(1):79-97. doi:10.18016/ksutarimdoga.vi.1699900