First Report of Rhodocybe fumanellii (Entolomataceae) from Türkiye with Morphological and Molecular Characterisation

Gülce Ediş; Eda Kumru; İsmail Acar; Halide Karabıyık; Ilgaz Akata

doi:10.18016/ksutarimdoga.vi.1703181

EN TR

First Report of Rhodocybe fumanellii (Entolomataceae) from Türkiye with Morphological and Molecular Characterisation

Abstract

This study presents a comprehensive taxonomic assessment of fungal specimens collected from Çanakkale Province in northwestern Türkiye. An integrative approach was employed, combining detailed macro and micromorphological analyses with molecular phylogenetic methods based on the internal transcribed spacer (nrITS,rDNA) region of nuclear ribosomal DNA. The results confirmed the identity of the specimens as Rhodocybe fumanellii R.J. Ferrari, Vizzini & Fellin, representing the first record of this species in Türkiye. As a newly documented member of section Rufobrunnea, this finding broadens the known geographical distribution of the species and contributes to understanding the genus Rhodocybe in the region. The study includes thorough morphological descriptions, ecological data, precise locality information with geographic coordinates, collection details, and photographic documentation, thereby enriching the knowledge of fungal biodiversity in Türkiye.

Keywords

Rhodocybe fumanellii (Entolomataceae)’nin Türkiye'deki İlk Kaydı ve Morfolojik-Moleküler Analizi

Öz

Çalışma, Türkiye'nin kuzeybatısındaki Çanakkale ilinden toplanan mantar örneklerinin kapsamlı bir taksonomik değerlendirmesini sunmaktadır. Ayrıntılı makro ve mikromorfolojik analizler ile nükleer ribozomal DNA'nın iç transkripsiyonlu spacer (nrITS, rDNA) bölgesine dayanan moleküler filogenetik yöntemleri birleştiren bütünleştirici bir yaklaşım kullanılmıştır. Sonuçlar, örneklerin Rhodocybe fumanellii R.J. Ferrari, Vizzini & Fellin olarak kimliğini doğrulamış ve bu türün Türkiye'deki ilk kaydını temsil etmiştir. Rufobrunnea bölümünün yeni belgelenmiş bir üyesi olarak bu bulgu, türün bilinen coğrafi dağılımını genişletmekte ve bölgedeki Rhodocybe cinsinin anlaşılmasına katkıda bulunmaktadır. Çalışma, kapsamlı morfolojik tanımları, ekolojik verileri, coğrafi koordinatlarla birlikte kesin lokalite bilgilerini, toplama detaylarını ve fotoğrafik dokümantasyonu içermekte ve böylece Türkiye'deki mantar biyoçeşitliliği bilgisini zenginleştirmektedir.

Anahtar Kelimeler

References

Akata, I. & Erdoğdu, M. (2020). First report of Rutstroemia elatina (Ascomycota) from Turkey. Kahramanmaraş Sütçü İmam University Journal of Agriculture and Nature 23 (2), 391-395. https://doi.org/10.18016/ksutarimdoga.vi.626466
Akata, I., Ediş, G., Kumru, E., Acar, İ., & Şahin, E. (2024a). Taxonomic Studies on Rhodocybe asyae Specimens Discovered in a New Location. Kahramanmaraş Sütçü İmam University Journal of Agriculture and Nature 27 (Suppl 1), 124-132. https://doi.org/10.18016/ksutarimdoga.vi.1507554
Akata, I., Kumru, E., Sahin, E., Acar, İ., & Kaya, E. (2024b). Amanita vidua: A new record for Turkish Amanita Section Phalloideae based on morphological and molecular data. Trakya University Journal of Natural Sciences, 25(1), 97-110. https://doi.org/10.23902/trkjnat.1446327
Akata, I., Ediş, G., Kumru, E., Acar, İ., & Şahin, E. (2024c). Initial Report of Inocybe costinitii in Türkiye with Morphological and Molecular Data. Kahramanmaraş Sütçü İmam University Journal of Agriculture and Nature 27 (6), 1304-1312. https://doi.org/10.18016/ksutarimdoga.vi.1467628
Akata, I., Kumru, E., Ediş, G., Acar, İ., & Sahin, E. (2024d). Two Newly Reported Agaricales Species from Türkiye with Morphological and Molecular Data. Kastamonu University Journal of Forestry Faculty, 24(3), 260-280. https://doi.org/10.17475/kastorman.1599952
Akata, I., Kumru, E., Ediş, G., Özbey, B. G., Acar, İ., & Sahin, E. (2025). Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye. Kahramanmaraş Sütçü İmam University Journal of Agriculture and Nature, 28(3), 843-854. https://doi.org/10.18016/ksutarimdoga.vi.1622161
Aplin, N., Cullington, P., Douglas, B., & Janke, E. (2022). DNA barcoding reveals three Rhodocybe species new to Britain. Field Mycology, 23(2), 41-47.
Crous, P. W., Wingfield, M. J., Burgess, T. I., Hardy, G., Barber, P. A. Alvarado, P., Barnes, C. W., Buchanan, P. K., Heykoop, M., Moreno, G., Thangavel, R., Van der spuy, S., Barili, A., Barrett, S., Cacciola, S.O., Cano-Lira, J.F., Crane, C., Decock, C., Gibertoni, T.B., Guarro, J., Guevara-Suarez, M., Hubka, V., Kolařík, M., Lira, C.R.S., ...................................... Groenewald, J.Z. (2017). Fungal Planet description sheets: 558–624. Persoonia: Molecular Phylogeny and Evolution of Fungi, 38, 240. https://doi.org/10.3767/003158517X698941

Dutta, A. K., Gates, G. M., Rakshit, S., & Acharya, K. (2021). Rhodocybe brunneoaurantiaca (sect. Rufrobrunnea, Entolomataceae): a new species from India. Nordic Journal of Botany, 39(6). https://doi.org/10.1111/njb.03061
Kaygusuz, O. (2024). Rhodocybe asanii (Entolomataceae: Agaricales), Türkiye için yeni bir lokalite kaydı. 2nd International Conference on Trends in Advanced Research (ICTAR 2024), Konya, Türkiye, November 22-23, 2024, pp. 707-712.
Kluting K. L., Baroni T. J., & Bergemann S. E. (2014). Toward a stable classification of genera within the Entolomataceae: a phylogenetic re-evaluation of the Rhodocybe-Clitopilus clade. Mycologia 106(6), 1127-1142. https://doi.org/10.3852/13-270
Lücking, R., Aime, M. C., Robbertse, B., Miller, A. N., Aoki, T. Ariyawansa, H. A., Cardinali, G., Crous, P. W., Druzhinina, I. S., Geiser, D. M., Hawksworth, D. L., Hyde, K. D., Irinyi, L., Jeewon, R., Johnston, P. R., Kirk, P. M., Malosso, E., May, T. W., Meyer, W., Nilsson, H.R., Öpik, M., Robert, V., Stadler, M., Thines, M., Vu, D., Yurkov, A.M., Zhang, N., & Schoch, C.L. (2021). Fungal taxonomy and sequence-based nomenclature. Nature Microbiology, 6(5), 540-548. https://doi.org/10.1038/s41564-021-00888-x
Raja, H. A., Miller, A. N., Pearce, C. J., & Oberlies, N. H. (2017). Fungal identification using molecular tools: a primer for the natural products research community. Journal of natural products, 80(3), 756-770. https://doi.org/10.1021/acs.jnatprod.6b01085
Reschke, K., Noordeloos, M. E., Manz, C., Hofmann, T. A., Rodríguez-Cedeño, J., Dima, B., & Piepenbring, M. (2022). Fungal diversity in the tropics: Entoloma spp. in Panama. Mycological progress, 21(1), 93-145. https://doi.org/10.1007/s11557-021-01752-2
Schoch, C. L., Seifert, K. A., Huhndorf, S., Robert, V., Spouge, J. L., Levesque, C. A., Chen, W., Bolchacova, K., ……………………………………….. , D. Schindel, D. 2012. “Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi”, Proceedings of the national academy of Sciences, 109(16), 6241-6246.. https://doi.org/10.1073/pnas.1117018109
Schoch, C. L., Robbertse, B., Robert, V., Vu, D., Cardinali, G., Irinyi, L., Meyer, W., Nilsson, R. H., Hughes, K., …………………………………………………….. Federhen, S. (2014). Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi. Database, bau061. https://doi.org/10.1093/database/bau061
Sesli, E. (2021). Rhodocybe cistetorum (Basidiomycota, Entolomataceae), a new species from the Colchic ecoregion of Turkey. Nordic Journal of Botany, 39(4). https://doi.org/10.1111/njb.03078
Sesli, E. & Vizzini, A. (2017). Two new Rhodocybe species (sect. Rufobrunnea, Entolomataceae) from the east black sea coast of Turkey. Turkish Journal of Botany, 41(2), 200-210. https://doi.org/10.3906/bot-1607-1
Silva-Filho, A. G., Baroni, T. J., Komura, D. L., Moncalvo, J. M., Baseia, I. G., & Wartchow, F. (2020). Rhodocybe fusipes (Entolomataceae), a new species from Amazonian ‘terra-firme’forest of Brazil. Sydowia, 72, 163-170. http://doi.org/10.12905/0380.sydowia72-2020-0163
Sleiman, S., Bellanger, J. M., Richard, F., & Stephan, J. (2021). First molecular-based contribution to the checklist of Lebanon macrofungi. Mycotaxon, 136(3), 687-687. https://doi.org/10.5248/136.687
Sun, Y. L. & Bau, T. (2023). Rhodocybe subasyae, a new species of Rhodocybe sect. Rufobrunnea (Entolomataceae, Agaricales) from northeast China. Mycoscience, 64(3), 96-100. https://doi.org/10.47371/mycosci.2023.03.001
Tamura, K. (1992). Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+C-content biases. Molecular Biology and Evolution, 9(4), 678-687. https://doi.org/10.1093/oxfordjournals.molbev.a040752
Tamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38(7), 3022-3027. https://doi.org/10.1093/molbev/msab120
Vizzini, A., Baroni T. J., Sesli, E., Antonín, V., & Saar, I. (2016). Rhodocybe tugrulii (Agaricales, Entolomataceae), a new species from Turkey and Estonia based on morphological and molecular data, and a new combination in Clitocella (Entolomataceae). Phytotaxa, 267, 1-15. https://doi.org/10.11646/phytotaxa.267.1.1
Vizzini, A., Ferrari, R. J., Ercole, E., & Fellin, A. (2018). A new species of Rhodocybe sect. Rufobrunnea (Entolomataceae, Agaricales) from Italy. MycoKeys, 36, 21-33. https://doi.org/10.3897/mycokeys.36.27094
Vizzini, A., Consiglio, G., & Marchetti, M. (2023). Overview of the European species of the genus Clitocella (Entolomataceae, Agaricales) with notes on extralimital taxa. Persoonia-Molecular Phylogeny and Evolution of Fungi, 50(1), 123-157. https://doi.org/10.3767/persoonia.2023.50.04
White, T. J., Bruns, T., Lee, S. J. W. T., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications, 18(1), pp. 315-322.

Details

Primary Language

English

Subjects

Botany (Other)

Journal Section

Research Article

Authors

Gülce Ediş
0000-0001-7038-9865
Türkiye

Eda Kumru
0009-0000-7417-6197
Türkiye

İsmail Acar
0000-0002-6049-4896
Türkiye

Halide Karabıyık
0000-0002-1778-2200
Türkiye

Ilgaz Akata ^*
0000-0002-1731-1302
Türkiye

Early Pub Date

July 25, 2025

Publication Date

September 11, 2025

Submission Date

May 21, 2025

Acceptance Date

July 9, 2025

Published in Issue

Year 2025 Volume: 28 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1703181

IZ

https://izlik.org/JA45XY22YR

Cite

RIS / Bibtex

APA

Ediş, G., Kumru, E., Acar, İ., Karabıyık, H., & Akata, I. (2025). First Report of Rhodocybe fumanellii (Entolomataceae) from Türkiye with Morphological and Molecular Characterisation. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(5), 1197-1205. https://doi.org/10.18016/ksutarimdoga.vi.1703181

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