Assessment of Genetic Diversity in Turkish Safflower (Carthamus tinctorius L.) Genotypes Using iPBS Retrotransposon Markers

Serap Demirel; Leyla Kurgan; Fatih Demirel; Barış Eren

doi:10.18016/ksutarimdoga.vi.1728043

TR EN

Türk Aspir (Carthamus tinctorius L.) Genotiplerinde Genetik Çeşitliliğin iPBS Retrotranspozon Belirteçleri Kullanılarak Değerlendirilmesi

Öz

Aspir (Carthamus tinctorius L.), tarımsal ve tıbbi açıdan önemli, kuraklığa dayanıklı bir yağlı tohum bitkisidir. Genetik çeşitliliğinin anlaşılması, geliştirilmiş çeşitlerin elde edilmesi ve yerel gen kaynaklarının korunması açısından büyük önem taşımaktadır. Bu çalışmada, Türkiye’ye ait 8 kültür çeşidi ve 25 yerel çeşit olmak üzere toplam 33 aspir genotipi, 12 inter-primer binding site (iPBS) retrotranspozon markörü kullanılarak karakterize edilmiştir. Toplam 124 bant amplifiye edilmiş ve bunların 120’si (%96.77) polimorfik bulunmuştur. Primer başına alel sayısı 4 ile 22 arasında değişmiş olup, ortalama 10 olarak belirlenmiştir. Ortalama Polimorfik Bilgi İçeriği (PIC) 0.19, etkili alel sayısı (Ne) 1.30, Shannon Bilgi İndeksi (I) 1.31 ve Çözünürlük Gücü (Rp) ise 7.01 olarak tespit edilmiştir. Jaccard benzerlik katsayılarına dayalı kümeleme analizi, genotipleri UPGMA yöntemi ile dört ana kümeye ayırmış ve benzerlik değerleri 0.24 ile 0.85 arasında değişmiştir. Temel Koordinat Analizi (PCoA), ilk iki koordinatın toplam varyasyonun %68.21’ini açıklamasıyla kümeleme sonuçlarını desteklemiştir. PCoA ve UPGMA desenleri arasındaki uyum, iPBS markörlerinin aspir genotiplerini ayırt etmedeki güvenilirliğini doğrulamaktadır. Genel olarak, elde edilen bulgular, iPBS retrotranspozon markörlerinin Türk aspir materyali arasında anlamlı genetik çeşitliliği ortaya koymadaki etkinliğini göstermekte ve ıslah çalışmaları ile gen kaynaklarının yönetimi için değerli bir moleküler kaynak sunmaktadır.

Anahtar Kelimeler

Assessment of Genetic Diversity in Turkish Safflower (Carthamus tinctorius L.) Genotypes Using iPBS Retrotransposon Markers

Abstract

Safflower (Carthamus tinctorius L.) is a drought-tolerant oilseed crop of agronomic and medicinal importance. Understanding its genetic diversity is crucial for developing improved cultivars and conserving local germplasm. In this study, 33 safflower genotypes, including 8 cultivars and 25 landraces from Türkiye, were characterized using 12 inter-primer binding site (iPBS) retrotransposon markers. A total of 124 bands were amplified, of which 120 (96.77%) were polymorphic. The number of alleles per primer ranged from 4 to 22, with an average of 10. Mean polymorphic information content (PIC), effective number of alleles (Ne), Shannon’s information index (I), and resolving power (Rp) were determined as 0.19, 1.30, 1.31, and 7.01, respectively. Cluster analysis based on Jaccard similarity coefficients grouped the genotypes into four major clusters using the UPGMA method, with a similarity range from 0.24 to 0.85. Principal Coordinate Analysis (PCoA) supported the clustering pattern, with the first two coordinates explaining 68.21% of the total genetic variation. The congruence between PCoA and UPGMA patterns confirms the robustness of the iPBS markers in discriminating safflower genotypes. Overall, the results demonstrate the effectiveness of iPBS markers in revealing significant genetic diversity among Turkish safflower accessions, providing a valuable molecular resource for breeding programs and germplasm management.

Keywords

References

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Details

Primary Language

English

Subjects

Agricultural Biotechnology (Other)

Journal Section

Research Article

Authors

Serap Demirel
0000-0002-1877-0797
Türkiye

Leyla Kurgan ^*
0000-0002-2052-6013
Türkiye

Fatih Demirel
0000-0002-6846-8422
Türkiye

Barış Eren
0000-0002-3852-6476
Türkiye

Early Pub Date

February 27, 2026

Publication Date

February 27, 2026

Submission Date

June 26, 2025

Acceptance Date

October 30, 2025

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1728043

IZ

https://izlik.org/JA67CZ66PT

Cite

RIS / Bibtex

APA

Demirel, S., Kurgan, L., Demirel, F., & Eren, B. (2026). Assessment of Genetic Diversity in Turkish Safflower (Carthamus tinctorius L.) Genotypes Using iPBS Retrotransposon Markers. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication, 955-967. https://doi.org/10.18016/ksutarimdoga.vi.1728043