Effect of the Bioactive Nutritional Component Piperlongumine on Histone Deacetylase Enzyme Activity in Human Cancer and Normal Cells

Sumeyye Bora; Hande Akalan; Duygu Yaşar Şirin; Türker Bilgen

doi:10.18016/ksutarimdoga.vi.1735140

EN TR

Effect of the Bioactive Nutritional Component Piperlongumine on Histone Deacetylase Enzyme Activity in Human Cancer and Normal Cells

Abstract

Currently, the effects of various bioactive compounds on Histone Deacetylase (HDAC) enzyme activity are being investigated. In this context, the aim of this study was to examine the effect of Piperlongumine (PL) on HDAC enzyme activity in HeLa and HEK293T cells in vitro. The IC₅₀ doses of PL for HeLa and HEK293T cell lines were determined using MTT and AO/PI staining methods. HDAC activity was measured using a commercial kit in lysates obtained via the NETN lysis method from cells treated with IC₅₀ doses of PL for 24 and 48 hours in vitro. Additionally, HDAC activity was assessed in lysates from cells cultured under normal conditions and directly treated with IC₅₀ doses of PL immediately before the HDAC assay. After 24- and 48-hour PL treatments, a statistically significant increase in HDAC activity was observed in HEK293T cells, whereas a non-significant increase was found in HeLa cells. In lysates directly treated with PL, HDAC activity significantly increased in HeLa cells but showed no significant change in HEK293T cells. These findings suggest that PL may affect HDACs via distinct mechanisms depending on cell type. The significant increase in HDAC activity upon direct treatment of HeLa lysates suggests a possible physical interaction between PL and HDACs in cancer cells. Conversely, the increased HDAC activity observed in HEK293T cells following 24- and 48-hour in vitro PL treatment implies that PL may act in normal cells through indirect and delayed pathways.

Keywords

Biyoaktif Besin Bileşeni Piperlongumin'in İnsan Kanser ve Normal Hücrelerinde Histon Deasetilaz Enzim Aktivitesi Üzerine Etkisi

Abstract

Günümüzde çeşitli biyoaktif bileşiklerin Histon Deasetilaz (HDAC) enzim aktivitesi üzerindeki etkileri araştırılmaktadır. Bu bağlamda, bu çalışmanın amacı Piperlongumine (PL)’in HeLa ve HEK293T hücrelerinde in vitro HDAC enzim aktivitesi üzerindeki etkisini incelemektir. HeLa ve HEK293T hücre hatları için PL’in IC₅₀ dozları MTT ve AO/PI boyama yöntemleri kullanılarak belirlenmiştir. HDAC aktivitesi, 24 ve 48 saat boyunca in vitro olarak IC₅₀ dozunda PL ile muamele edilen hücrelerden NETN lizis yöntemiyle elde edilen lizatlarda ticari bir kit kullanılarak ölçülmüştür. Ayrıca, normal koşullarda kültüre edilen ve HDAC aktivite analizinden hemen önce IC₅₀ dozunda PL ile doğrudan muamele edilen hücrelerden elde edilen lizatlarda da HDAC aktivitesi değerlendirilmiştir. 24 ve 48 saatlik PL uygulamaları sonrasında, HEK293T hücrelerinde HDAC aktivitesinde istatistiksel olarak anlamlı bir artış gözlenmiş, HeLa hücrelerinde ise anlamlı olmayan bir artış saptanmıştır. PL ile doğrudan muamele edilen lizatlarda ise, HeLa hücrelerinde HDAC aktivitesi anlamlı düzeyde artarken, HEK293T hücrelerinde anlamlı bir değişiklik gözlenmemiştir. Bu bulgular, PL’in hücre tipine bağlı olarak HDAC’leri farklı mekanizmalarla etkileyebileceğini düşündürmektedir. HeLa hücre lizatlarında gözlenen HDAC aktivite artışı, PL’in kanser hücrelerinde HDAC’lerle fiziksel etkileşim yoluyla etkili olabileceğini göstermektedir. Buna karşılık, kültür ortamında24 ve 48 saat süreyle PL ile muamele edilen HEK293T hücrelerinde gözlenen aktivite artışı, PL’in normal hücrelerde dolaylı ve gecikmeli yollarla etkili olabileceğini düşündürmektedir.

Keywords

References

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Sumeyye Bora
0000-0002-5807-4788
Türkiye

Hande Akalan
0000-0002-5922-2498
Türkiye

Duygu Yaşar Şirin
0000-0002-1224-442X
Türkiye

Türker Bilgen ^*
0000-0002-3015-0929
Türkiye

Early Pub Date

September 19, 2026

Publication Date

-

Submission Date

July 7, 2025

Acceptance Date

February 17, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1735140

IZ

https://izlik.org/JA22SR63AP

Cite

RIS / Bibtex

APA

Bora, S., Akalan, H., Yaşar Şirin, D., & Bilgen, T. (2026). Effect of the Bioactive Nutritional Component Piperlongumine on Histone Deacetylase Enzyme Activity in Human Cancer and Normal Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication, 1-8. https://doi.org/10.18016/ksutarimdoga.vi.1735140

AMA

1.Bora S, Akalan H, Yaşar Şirin D, Bilgen T. Effect of the Bioactive Nutritional Component Piperlongumine on Histone Deacetylase Enzyme Activity in Human Cancer and Normal Cells. KSU J. Agric Nat. 2026;(Advanced Online Publication):1-8. doi:10.18016/ksutarimdoga.vi.1735140

Chicago

Bora, Sumeyye, Hande Akalan, Duygu Yaşar Şirin, and Türker Bilgen. 2026. “Effect of the Bioactive Nutritional Component Piperlongumine on Histone Deacetylase Enzyme Activity in Human Cancer and Normal Cells”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication: 1-8. https://doi.org/10.18016/ksutarimdoga.vi.1735140.

EndNote

Bora S, Akalan H, Yaşar Şirin D, Bilgen T (September 1, 2026) Effect of the Bioactive Nutritional Component Piperlongumine on Histone Deacetylase Enzyme Activity in Human Cancer and Normal Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication 1–8.

IEEE

[1]S. Bora, H. Akalan, D. Yaşar Şirin, and T. Bilgen, “Effect of the Bioactive Nutritional Component Piperlongumine on Histone Deacetylase Enzyme Activity in Human Cancer and Normal Cells”, KSU J. Agric Nat., no. Advanced Online Publication, pp. 1–8, Sept. 2026, doi: 10.18016/ksutarimdoga.vi.1735140.

ISNAD

Bora, Sumeyye - Akalan, Hande - Yaşar Şirin, Duygu - Bilgen, Türker. “Effect of the Bioactive Nutritional Component Piperlongumine on Histone Deacetylase Enzyme Activity in Human Cancer and Normal Cells”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (September 1, 2026): 1-8. https://doi.org/10.18016/ksutarimdoga.vi.1735140.

JAMA

1.Bora S, Akalan H, Yaşar Şirin D, Bilgen T. Effect of the Bioactive Nutritional Component Piperlongumine on Histone Deacetylase Enzyme Activity in Human Cancer and Normal Cells. KSU J. Agric Nat. 2026;:1–8.

MLA

Bora, Sumeyye, et al. “Effect of the Bioactive Nutritional Component Piperlongumine on Histone Deacetylase Enzyme Activity in Human Cancer and Normal Cells”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication, Sept. 2026, pp. 1-8, doi:10.18016/ksutarimdoga.vi.1735140.

Vancouver

1.Sumeyye Bora, Hande Akalan, Duygu Yaşar Şirin, Türker Bilgen. Effect of the Bioactive Nutritional Component Piperlongumine on Histone Deacetylase Enzyme Activity in Human Cancer and Normal Cells. KSU J. Agric Nat. 2026 Sep. 1;(Advanced Online Publication):1-8. doi:10.18016/ksutarimdoga.vi.1735140