Identification of Bacterial Isolates with Antagonistic Activity Against Pseudomonas syringae pv. atrofaciens, the Causal Agent of Basal Glume Rot in Wheat, and Their Plant Growth-Promoting Traits

Murat Öztürk

doi:10.18016/ksutarimdoga.vi.1756518

EN TR

Identification of Bacterial Isolates with Antagonistic Activity Against Pseudomonas syringae pv. atrofaciens, the Causal Agent of Basal Glume Rot in Wheat, and Their Plant Growth-Promoting Traits

Abstract

In this study, bacterial isolates exhibiting antagonistic properties against the seed-borne pathogen Pseudomonas syringae pv. Atrofaciens in wheat were investigated. Among a total of 298 bacterial isolates obtained from wheat seed samples, 19 candidate antagonistic isolates were selected based on their pectolytic activity and hypersensitivity test results. Molecular identification revealed that these isolates belonged to the genera Pseudomonas, Bacillus, Paenibacillus, Citrobacter, and Staphylococcus. In vitro antagonistic tests showed that Pseudomonas japonica isolates W57.4 and W57.5, along with Paenibacillus polymyxa isolate W58.1, exhibited the highest inhibitory effects. These isolates also demonstrated multiple plant growth-promoting traits such as protease enzyme production, phosphate solubilization, siderophore synthesis, ammonia production, and indole-3-acetic acid (IAA) production. Notably, P. japonica isolates (W57.4, W57.5) exhibited strong siderophore production and phosphate solubilization, while P. Polymyxa (W58.1) showed high protease activity. These multifunctional traits indicate the potential of these isolates to be used as biological control agents against seed-borne pathogens. As the first study in Türkiye focusing on the biological control of seed-borne bacterial pathogens causing basal glume rot in wheat, this research provides a significant contribution to the development of environmentally friendly and effective alternative management strategies.

Keywords

Buğday Kavuz Dibi Çürüklüğü Hastalığına Neden Olan Pseudomonas syringae pv. atrofaciens’e Karşı Antagonist Etkili ve Bitki Gelişimini Teşvik Edici Bakteri İzolatlarının Belirlenmesi

Öz

Bu çalışmada, buğdayda tohum kaynaklı hastalık etmeni Pseudomonas syringae pv. atrofaciens’e karşı antagonistik özellik gösteren bakteri izolatları araştırılmıştır. Buğday tohum örneklerinden izole edilen toplam 298 bakteri izolatı arasından pektolitik aktivite ve hipersensitivite testi sonuçlarına göre 19 aday antagonistik izolat seçilmiştir. Moleküler tanımlama ile bu izolatların Pseudomonas, Bacillus, Paenibacillus, Citrobacter ve Staphylococcus cinslerine ait olduğu belirlendi.İn vitro antagonistik testlerde, Pseudomonas japonica izolatları W57.4 ve W57.5 ile Paenibacillus polymyxa izolatı W58.1 en yüksek inhibitör etkiyi göstermiştir. Bu izolatlar ayrıca proteaz enzimi üretimi, fosfat çözünürlüğü, siderofor sentezi, amonyak ve indol-3-asetik asit (IAA) üretimi gibi çoklu bitki gelişimini destekleyen özellikler sergilemiştir. Özellikle P. japonica izolatları (W57.4, W57.5) güçlü siderofor üretimi ve fosfat çözünürlüğü gösterirken, P. polymyxa (W58.1) yüksek proteaz aktivitesiyle dikkat çekmiştir. Bu çok işlevli özellikler, söz konusu izolatların tohum kaynaklı patojenlerin kontrolünde biyolojik mücadele ajanı olarak kullanılma potansiyelini ortaya koymaktadır. Türkiye'de buğdayda tohum kaynaklı bakteriyel patojen olan kavuz dibi çürüklüğünün biyolojik kontrolüne yönelik gerçekleştirilen ilk çalışma olması bakımından, bu araştırma çevre dostu ve etkili alternatif mücadele yöntemlerinin geliştirilmesine önemli bir katkı sunmaktadır.

Anahtar Kelimeler

Supporting Institution

The Scientific Research Projects Commission of Yozgat Bozok University

Project Number

FHD-2024-1362

Ethical Statement

This article lacks any study related to human or animal participants performed by any of the authors.

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Details

Primary Language

English

Subjects

Phytopathology

Journal Section

Research Article

Authors

Murat Öztürk ^*
0000-0002-9677-3651
Türkiye

Early Pub Date

February 8, 2026

Publication Date

February 8, 2026

Submission Date

August 1, 2025

Acceptance Date

October 15, 2025

Published in Issue

Year 2026 Volume: 29 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1756518

IZ

https://izlik.org/JA44YR34HA

Cite

RIS / Bibtex

APA

Öztürk, M. (2026). Identification of Bacterial Isolates with Antagonistic Activity Against Pseudomonas syringae pv. atrofaciens, the Causal Agent of Basal Glume Rot in Wheat, and Their Plant Growth-Promoting Traits. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 29(3), 665-677. https://doi.org/10.18016/ksutarimdoga.vi.1756518