Newly Reported Elaphomyces Species from Kırklareli, Türkiye: A Morphological and Molecular Approach

Gülce Ediş; Eda Kumru; Emre Keskin; Ergin Sahin; Ilgaz Akata

doi:10.18016/ksutarimdoga.vi.1767264

EN TR

Newly Reported Elaphomyces Species from Kırklareli, Türkiye: A Morphological and Molecular Approach

Abstract

This study aims to enhance our understanding of Elaphomyces diversity in Türkiye by combining morphological and molecular techniques to document newly discovered species. Fungal samples were collected from Kırklareli Province between October and December 2022. Their morphological characteristics were examined using light microscopy and scanning electron microscopy, while molecular phylogenetic analyses of nrITS (rDNA) sequences provided complementary evidence for species identification. The research identified three new Turkish collections: Elaphomyces aculeatus, E. americanus, and E. virgatosporus, whose ITS sequences showed over 99% similarity to corresponding entries in the GenBank database. Additionally, this study provides detailed morphological descriptions, habitat information, and phylogenetic analyses.

Keywords

Supporting Institution

TUBİTAK

Project Number

121Z924

Thanks

The study was supported by the Turkish Scientific and Technological Research Council (TÜBİTAK) with project number 121Z924.

References

Akata, I., Ediş, G., Kumru, E., Acar, İ., & Şahin, E. (2024a). Taxonomic Studies on Rhodocybe asyae Specimens Discovered in a New Location. Kahramanmaraş Sütçü İmam University Journal of Agriculture and Nature 27 (Suppl 1), 124–132. https://doi.org/10.18016/ksutarimdoga.vi.1507554
Akata, I., Kumru, E., Sahin, E., Acar, İ., & Kaya, E. (2024b). Amanita vidua: A new record for Turkish Amanita Section Phalloideae based on morphological and molecular data. Trakya University Journal of Natural Sciences, 25(1), 97–110. https://doi.org/10.23902/trkjnat.1446327
Akata, I., Ediş, G., Kumru, E., Acar, İ., & Şahin, E. (2024c). Initial Report of Inocybe costinitii in Türkiye with Morphological and Molecular Data. Kahramanmaraş Sütçü İmam University Journal of Agriculture and Nature 27 (6), 1304–1312. https://doi.org/10.18016/ksutarimdoga.vi.1467628
Akata, I., Kumru, E., Ediş, G., Acar, İ., & Sahin, E. (2024d). Two Newly Reported Agaricales Species from Türkiye with Morphological and Molecular Data. Kastamonu University Journal of Forestry Faculty, 24(3), 260–280. https://doi.org/10.17475/kastorman.1599952
Akata, I., Kumru, E., Ediş, G., Özbey, B. G., Acar, İ., & Şahin, E. (2025a). Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye. Kahramanmaraş Sütçü İmam University Journal of Agriculture and Nature 28(3), 843–854. https://doi.org/10.18016/ksutarimdoga.vi.1622161
Akata, I., Ediş, G., Kumru, E., Keskin, E., & Şahin, E. (2025b). Statesia pompholyx: A Newly Reported Species for Turkish Hysterangiales. Kastamonu University Journal of Forestry Faculty, 25(1), 1–8. https://doi.org/10.17475/kastorman.1660474
Allen, K. & Bennett, J. W. (2021). Tour of truffles: aromas, aphrodisiacs, adaptogens, and more. Mycobiology, 49(3), 201–212. https://doi.org/10.1080/12298093.2021.1936766
Bach C., Beacco P., Cammaletti P., Babel-Chen, Z., Levesque, E., Todesco, F., Cotton, C., Robin, B., & Murat, C. (2021). First production of Italian white truffle (Tuber magnatum Pico) ascocarps in an orchard outside its naturalrange distribution in France. Mycorrhiza, 31(3), 383–388. https://doi.org/10.1007/s00572-020-01013-2

Bonito, G., Trappe, J. M., Rawlinson, P., & Vilgalys, R. (2010). Improved resolution of major clades within Tuber and taxonomy of species within the Tuber gibbosum complex. Mycologia, 102(5), 1042–1057. https://doi.org/10.3852/09-213
Bratek, Z., Merényi, Z., & Varga, T. (2013). Changes of hypogeous fungi in the Carpathian Pannonian region in the past centuries. Acta Mycologica, 48(1), 33–39. https://doi.org/10.5586/am.2013.005
Buyck, B., Hosaka, K., Masi, S., & Hofstetter, V. (2016). Molecular analyses of first collections of Elaphomyces Nees (Elaphomycetaceae, Eurotiales, Ascomycota) from Africa and Madagascar indicate that the current concept of Elaphomyces is polyphyletic. Cryptogamie, Mycologie, 37(1), 3–14. https://doi.org/10.7872/crym/v37.iss1.2016.3
Cabero, A., Alvarado, P., Alonson, J., Salcedo, I., & Moreno-Mateos, D. (2019). Elaphomices lilacinus sp. nov (Ascomycota, Eurotiomycetes), a new hypogeous species from Navarre (Spain). Ascomycete.org, 11:229–232. https://doi.org/10.25664/art-0279
Castellano, M. A., Trappe, J. M., & Vernes, Karl. (2011). Australian species of Elaphomyces (Elaphomycetaceae, Eurotiales, Ascomycota). Australian Systematic Botany, 24, 32–57. https://doi.org/10.1071/SB10012
Castellano, M. A., Beever, R. E., & Trappe, J. M. (2012a). Sequestrate fungi of New Zealand: Elaphomyces (Ascomycota, Eurotiales, Elaphomycetaceae). New Zealand Journal of Botany, 50(4), 423–433. http://doi.org/10.1080/0028825X.2012.725057
Castellano, M. A., Henkel, T. W., Miller, S. L., Smith, M. E., & Aime, M. C. (2012b). New Elaphomyces species (Elaphomycetaceae, Eurotiales, Ascomycota) from Guyana. Mycologia, 104(5), 1244–1249. https://doi.org/10.3852/12-061
Castellano, M. A., Dentinger, B. T. M., Séné, O, Elliott, T. F., Truong, C., & Henkel, T. W. (2016). New species of Elaphomyces (Elaphomycetaceae, Eurotiales, Ascomycota) from tropical rainforests of Cameroon and Guyana. IMA Fungus 7, 59–73. https://doi.org/10.5598/imafungus.2016.07.01.05
Castellano, M. A. & Stephens, R. B. (2017). Elaphomyces species (Elaphomycetaceae, Eurotiales) from bartlett experimental forest, New Hampshire, USA. IMA fungus, 8(1), 49–63. https://doi.org/10.5598/imafungus.2017.08.01.04
Castellano, M. A., Elliott, T.F., & Trappe, J.M. (2018). Three new black Elaphomyces species (Elaphomycetaceae, Eurotiales, Ascomycota) from eastern North America with notes on selected European species. Fungal Systematics and Evolution, 1, 1–12. https://doi.org/10.3114/fuse.2018.01.01
Castellano, M. A., Crabtree, C. D., Mitchell, D., & Healy, R. A. (2021). Eight new Elaphomyces species (Elaphomycetaceae, Eurotiales, Ascomycota) from eastern North America. Fungal Systematics and Evolution, 7(1), 113–131. https://doi.org/10.3114/fuse.2021.07.06
Čejka, T., Thomas, P. W., Oliach, D., Stobbe, U., Egli, S., Tegel, W., Centenaro, G., Sproll, L., Bagi, I., Trnka, M., & Büntgen, U. (2022). Understanding the performance of Truffle Dogs. Journal of Veterinary Behavior, 52–53, 8–13. https://doi.org/10.1016/j.jveb.2022.04.002
Chachula, P., Fiedor, M., Rutkowski, R., & Dorda, A. (2020). New record of macrofungi for the mycobiota of the Cieszyn municipality (Polish Western Carpathians) including new species to Poland. Acta Mycologica, 55(1): 5511. https://doi.org/10.5586/am.5511
Crous, P. W., Wingfield, M. J., Chooi, Y. H., Gilchrist, C. L., Lacey, E., Pitt, J. I., Roets, F., Swart, W. J., Cano-Lira, J. F., Valenzuela-Lopez, N., Hubka, V., Shivas, R. G., Stchigel, A. M., Holdom, D. G., Jurjević, Ž., Kachalkin, A. V., Lebel, T., Lock, C., Martín, M. P., Tan, Y. P., Tomashevskaya, M. A., Vitelli, J. S., Baseia, I. G., Bhatt, V. K., Brandrud, T. E., De Souza, J. T., Dima, B., Lacey, H. J., Lombard, L., Johnston, P. R., Morte, A., Papp, V., Rodríguez, A., ..... Zmitrovich, I. V., Zvyagina, E. A., & Groenewald, J. Z. (2021). Fungal Planet description sheets: 1182–1283. Persoonia: Molecular Phylogeny and Evolution of Fungi, 46, 313. https://doi.org/10.3767/persoonia.2021.46.11
de la Fuente, J. I., García-Jiménez, J., Raymundo, T., Sánchez-Flores, M., Valenzuela, R., Guevara-Guerrero, G., Pérez-Ovando, E. C., & Martínez-González, C. R. (2023a) Elaphomyces castilloi (Elaphomycetaceae, Ascomycota) and Entoloma secotioides (Entolomataceae, Basidiomycota), two new sequestrate fungi from tropical montane cloud forest from south Mexico. MycoKeys 96, 127–142. https://doi.org/10.3897/mycokeys.96.98320
de la Fuente, J. I., Pérez-Moreno, J., Martínez-Reyes, M., Ayala-Vásquez, O., Martínez-González, C. R., & Aguirre-Acosta, C. E. (2023b). Elaphomyces readii (Elaphomycetaceae, Eurotiomycetes), a new medicinal species of hypogeous fungus with biocultural importance from Mexico. Phytotaxa, 594(4), 241–250. https://doi.org/10.11646/phytotaxa.594.4.1
Ediş, G., Kumru, E., Acar, İ., Karabıyık, H., & Akata, I. First Report of Rhodocybe fumanellii (Entolomataceae) from Türkiye with Morphological and Molecular Characterisation. Kahramanmaraş Sütçü İmam University Journal of Agriculture and Nature, 28(5), 1197–1205. https://doi.org/10.18016/ksutarimdoga.vi.1703181
Elliott, T. F., Truong, C., Jackson, S. M., Zúñiga, C. L., Trappe, J. M., & Vernes, K. (2022). Mammalian mycophagy: a global review of ecosystem interactions between mammals and fungi. Fungal Systematics and Evolution, 9(1), 99–159. https://doi.org/10.3114/fuse.2022.09.07
Gençer, F. & Yüksek, İ. (2022). Examination of the Diversity in Rural Architecture in Kırklareli Through Factors. International Journal of Architecture and Planning, 10(1), 299–324. https://doi.org/10.15320/ICONARP.2022.204
Kers, L. E. (1997). Elaphomyces virgatosporus funnen i Sverige. Svensk botanisk tidskrift, 91, 25–36. Kültür, Ş. (2007). Medicinal plants used in Kırklareli province (Turkey). Journal of Ethnopharmacology, 111(2), 341–364. https://doi.org/10.1016/j.jep.2006.11.035
Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549. https://doi.org/10.1093/molbev/msy096
Kumru, E., Ediş, G., Sahin, E., Keskin, E., & Akata, I. (2025). Expanding the diversity of Genea Vittad. (Ascomycota, Pezizales) in Türkiye: Morphological and molecular insights into newly recorded species. Trakya University Journal of Natural Sciences, (Online First). https://doi.org/10.23902/trkjnat.1640957
Læssøe, T., Jordal, J. B., Nielsen, J. G. B., Holtan, D., & Larsen, P. G. (2009). Elaphomyces virgatosporus in NW Norway–the northernmost records of a rare truffle. Agarica, 28, 43–49.
Molia, A., Larsson, E., Jeppson, M., Læssøe, T., & Larsson, K. H. (2020). Elaphomyces section Elaphomyces (Eurotiales, Ascomycota) taxonomy and phylogeny of North European taxa, with the introduction of three new species. Fungal Systematics and Evolution, 5(1), 283–300.https://doi.org/10.3114/fuse.2020.05.14
Morgül, Ş. (2014). Kırklareli İlinde Eko Turizm Olanakları. Electronic Journal of Vocational Colleges, 4(4), 27–38. https://doi.org/10.17339/ejovoc.75153
Olivier, J.-M., Savignac, J.-C., Sourzat, P., 2018. Truffe Et Trufficulture. Fanlac, France, p. 352.
Paz, A., Bellanger, J. M., Lavoise, C., Molia, A., Ławrynowicz, M., Larsson, E., Ibarguren, I. O., Jeppson, M., Læssøe, T., Sauve, M., Richard, F., & Moreau, P. A. (2017). The genus Elaphomyces (Ascomycota, Eurotiales): A ribosomal DNA-based phylogeny and revised systematics of European ‘deer truffles’. Persoonia, 38(1), 197–239. https://doi.org/10.3767/003158517X697309
Qu, H., Cai, Q., Redhead, S. A., Chen, X., Ge, Z. W., & Yang, Z. L. (2025). Exploring criteria for constructing suprageneric classifications of fungi in the genomic era: a case study of suborders Agaricineae, Pluteineae, and Tricholomatineae (Agaricales). Fungal Diversity, 1–24. https://doi.org/10.1007/s13225-025-00557-y
Raja, H. A., Miller, A. N., Pearce, C. J., & Oberlies, N. H. (2017). Fungal identification using molecular tools: a primer for the natural products research community. Journal of Natural Products, 80(3), 756–770. https://doi.org/10.1021/acs.jnatprod.6b01085
Rusbridge, C. & Wilkins, P. (2002). Neuronal heterotopia in a Lagotto Romagnolo dog. Neuropathology and Applied Neurobiology, 28(2), 162–162. https://doi.org/10.1046/j.1365-2990.2002.39286_39.x
Shirakawa, M. & Tanaka, M. (2020). Two new deer truffle species, Elaphomyces marmoratus and Elaphomyces fuscus spp. nov., from a secondary forest in Japan. Mycoscience, 61(6), 315–322. https://doi.org/10.1016/j.myc.2020.07.002 1340-3540
Siller, I., Vasas, G., Pál-Fám, F., Bratek, Z., Zagyva, I., & Fodor, L. (2005). Hungarian distribution of the legally protected macrofungi species. Studia botanica hungarica, 36, 131–163.
Sukarno, N., Listiyowati, S., Rahayu, N., & Nara, K. (2018). Elaphomyces tropicalis sp. nov.: A new ectomycorrhizal fungus associated with dipterocarps from tropical Indonesia. Mycoscience, 60(2), 83–88. https://doi.org/10.1016/j.myc.2018.12.004
Tamura, K., & Nei, M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10(3), 512-526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
Tan, Y. P., Bishop-Hurley, S. L., Shivas, R. G., Cowan, D. A., Maggs-Kölling, G., Maharachchikumbura, S. S., ....................... , Crous, P. W., & Marqués-Gálvez, J. E. (2022). Fungal Planet description sheets: 1436–1477. Persoonia-Molecular Phylogeny and Evolution of Fungi, 49(1), 261–350. https://doi.org/10.3767/persoonia.2022.49.08
Türkoğlu, A., Castellano, M. A., Trappe, J. M., & Yaratanakul Güngör M. 2015. Turkish truffles I: 18 new records for Turkey. Turkish Journal of Botany, 39(2): 359–376. https://doi.org/10.3906/bot-1406-42
Uzun, Y. (2021). Elaphomyces anthracinus, a new hypogeous ascomycete record for Turkish mycota. Anatolian Journal of Botany, 5 (1): 29–31. https://doi.org/10.30616/ajb.857752
Uzun, Y. & Kaya, A. (2019). Elaphomyces granulatus, A New Hypogeous Ascomycete Record for Turkey. Kahramanmaraş Sütçü İmam University Journal of Agriculture and Nature, 22(1): 85–88. https://doi.org/10.18016/ksutarimdoga.vi.466008
Uzun, Y. & Kaya, A. (2020). Elaphomyces citrinus and E. cyanosporus, new for Turkey. Mycotaxon, 135: 339–344. https://doi.org/10.5248/135.339
Uzun, Y. & Kaya A. (2021). First Record of Elaphomyces decipiens for the Mycobiota of Turkey. Mantar Dergisi, 12(2): 134–137. https://doi.org/10.30708.mantar955067
White, T. J., Bruns, T., Lee, S., & Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. pp. 315–322. In: Innis, M.A. & Gelfand, D.H. (eds). PCR Protocols: A Guide To Methods And Applications. Academic Press, London, 482 pp.
Yılmaz, M. U. (2023). Keşif kuraklık indeksi ve standartlaştırılmış yağış indeksi kullanılarak kırklareli ilinde kuraklığın eğilimi ve zamansal değişkenliği. Doğal Afetler ve Çevre Dergisi, 9(2), 341–364. https://doi.org/10.21324/dacd.1296428

Details

Primary Language

English

Subjects

Botany (Other)

Journal Section

Research Article

Authors

Gülce Ediş
0000-0001-7038-9865
Türkiye

Eda Kumru
0009-0000-7417-6197
Türkiye

Emre Keskin
0000-0002-7279-313X
Türkiye

Ergin Sahin
0000-0003-1711-738X
Türkiye

Ilgaz Akata ^*
0000-0002-1731-1302
Türkiye

Early Pub Date

January 21, 2026

Publication Date

January 21, 2026

Submission Date

August 17, 2025

Acceptance Date

September 19, 2025

Published in Issue

Year 1970 Volume: 29 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1767264

IZ

https://izlik.org/JA63KM25PS

Cite

RIS / Bibtex

APA

Ediş, G., Kumru, E., Keskin, E., Sahin, E., & Akata, I. (2026). Newly Reported Elaphomyces Species from Kırklareli, Türkiye: A Morphological and Molecular Approach. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 29(2), 353-369. https://doi.org/10.18016/ksutarimdoga.vi.1767264

Newly Reported Elaphomyces Species from Kırklareli, Türkiye: A Morphological and Molecular Approach

Newly Reported Elaphomyces Species from Kırklareli, Türkiye: A Morphological and Molecular Approach

Abstract

Keywords

Kırklareli, Türkiye'den Yeni Bildirilen Elaphomyces Türleri: Morfolojik ve Moleküler Bir Yaklaşım

Öz

Anahtar Kelimeler

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

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IZ

Cite