Microsatellite-Based Insights into Bottleneck Dynamics in Awassi Sheep

Zühal Gündüz; Onur Yılmaz; Nezih Ata; Sabri Gül; Mahmut Keskin; İbrahim Cemal; Orhan Karaca

doi:10.18016/ksutarimdoga.vi.1767827

TR EN

İvesi Koyunlarında Darboğaz Dinamiklerine Mikrosatellit Tabanlı Bakış

Abstract

İvesi koyunu, Türkiye’de özellikle süt verimi ve adaptasyon kabiliyeti bakımından stratejik öneme sahip yerli bir koyun ırkıdır. Bu çalışmada, Gaziantep ve Kilis illerinde yetiştirilen İvesi sürülerinden örneklenen 190 baş hayvan, 17 mikrosatellit lokusu kullanılarak genotiplendirilmiş ve popülasyonun genetik varyasyon düzeyi ile popülasyon yapısı değerlendirilmiştir. Toplam 306 allel belirlenmiş olup, lokus başına ortalama allel sayısı yüksek allelik zenginliğe işaret etmiştir. Ortalama gözlenen heterozigotluk (Ho = 0,72) değerinin beklenen heterozigotluk (He = 0,82) değerinden düşük olması, popülasyonda kısmi heterozigotluk eksikliği ve olası Wahlund etkisini düşündürmektedir. Sürü alt popülasyonları arasındaki genetik farklılaşma düzeyi oldukça düşük bulunmuş (FST = 0,019) ve bu sonuç popülasyonlar arası gen akışının sürdüğünü göstermiştir. Bayesian kümeleme analizi (STRUCTURE), coğrafi dağılımdan bağımsız olarak üç genetik alt kümelenmenin varlığını ortaya koymuştur. Bottleneck testleri, yakın dönemde etkili popülasyon büyüklüğünde (Ne) anlamlı bir daralma olmadığını göstermiştir. Bulgular, 2005 yılında başlatılan Halk Elinde Küçükbaş Hayvan Islahı Ülkesel Projesi kapsamında yürütülen seleksiyon programlarının genetik varyasyonu koruyacak şekilde uygulandığını ve popülasyonun uzun dönemli genetik sürdürülebilirliğinin sağlandığını göstermektedir.

Keywords

Project Number

ZRF-22008

Microsatellite-Based Insights into Bottleneck Dynamics in Awassi Sheep

Abstract

Awassi sheep is a strategically important indigenous breed in Türkiye, particularly valued for its dairy performance and environmental adaptability. In this study, 190 individuals sampled from flocks in the Gaziantep and Kilis provinces were genotyped using 17 microsatellite loci to characterize genetic variability and population structure. A total of 306 alleles were detected, indicating substantial allelic richness. The mean observed heterozygosity (Ho = 0.72) was lower than the expected heterozygosity (He = 0.82), suggesting low to moderate heterozygote deficiency potentially attributable to subpopulation structure (Wahlund effect). Genetic differentiation among flock subpopulations was low (FST = 0.019), indicating ongoing gene flow and weak population subdivision. Bayesian clustering analysis implemented in STRUCTURE identified three genetic clusters independent of strict geographic boundaries. Bottleneck analyses revealed no evidence of a recent reduction in effective population size (Ne). These findings indicate that genetic diversity has been effectively maintained and support the long-term sustainability of the selection scheme conducted under the “National Genetic Breeding Project in Small Ruminants under Breeders' Conditions” initiated in 2005.

Keywords

Project Number

ZRF-22008

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Details

Primary Language

English

Subjects

Animal Science, Genetics and Biostatistics

Journal Section

Research Article

Authors

Zühal Gündüz ^*
0000-0002-1427-1501
Türkiye

Onur Yılmaz
0000-0002-5658-8558
Türkiye

Nezih Ata
0000-0002-0387-5131
Türkiye

Sabri Gül
0000-0001-6787-8190
Türkiye

Mahmut Keskin
0000-0002-8147-2477
Türkiye

İbrahim Cemal
0000-0002-4069-4815
Türkiye

Orhan Karaca
0000-0002-7323-9173
Türkiye

Early Pub Date

September 20, 2026

Publication Date

-

Submission Date

August 18, 2025

Acceptance Date

May 11, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1767827

IZ

https://izlik.org/JA65TR27TY

Cite

RIS / Bibtex

APA

Gündüz, Z., Yılmaz, O., Ata, N., Gül, S., Keskin, M., Cemal, İ., & Karaca, O. (2026). Microsatellite-Based Insights into Bottleneck Dynamics in Awassi Sheep. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication, 242-256. https://doi.org/10.18016/ksutarimdoga.vi.1767827

AMA

1.Gündüz Z, Yılmaz O, Ata N, et al. Microsatellite-Based Insights into Bottleneck Dynamics in Awassi Sheep. KSU J. Agric Nat. 2026;(Advanced Online Publication):242-256. doi:10.18016/ksutarimdoga.vi.1767827

Chicago

Gündüz, Zühal, Onur Yılmaz, Nezih Ata, et al. 2026. “Microsatellite-Based Insights into Bottleneck Dynamics in Awassi Sheep”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication: 242-56. https://doi.org/10.18016/ksutarimdoga.vi.1767827.

EndNote

Gündüz Z, Yılmaz O, Ata N, Gül S, Keskin M, Cemal İ, Karaca O (September 1, 2026) Microsatellite-Based Insights into Bottleneck Dynamics in Awassi Sheep. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication 242–256.

IEEE

[1]Z. Gündüz et al., “Microsatellite-Based Insights into Bottleneck Dynamics in Awassi Sheep”, KSU J. Agric Nat., no. Advanced Online Publication, pp. 242–256, Sept. 2026, doi: 10.18016/ksutarimdoga.vi.1767827.

ISNAD

Gündüz, Zühal - Yılmaz, Onur - Ata, Nezih - Gül, Sabri - Keskin, Mahmut - Cemal, İbrahim - Karaca, Orhan. “Microsatellite-Based Insights into Bottleneck Dynamics in Awassi Sheep”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (September 1, 2026): 242-256. https://doi.org/10.18016/ksutarimdoga.vi.1767827.

JAMA

1.Gündüz Z, Yılmaz O, Ata N, Gül S, Keskin M, Cemal İ, Karaca O. Microsatellite-Based Insights into Bottleneck Dynamics in Awassi Sheep. KSU J. Agric Nat. 2026;:242–256.

MLA

Gündüz, Zühal, et al. “Microsatellite-Based Insights into Bottleneck Dynamics in Awassi Sheep”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication, Sept. 2026, pp. 242-56, doi:10.18016/ksutarimdoga.vi.1767827.

Vancouver

1.Zühal Gündüz, Onur Yılmaz, Nezih Ata, Sabri Gül, Mahmut Keskin, İbrahim Cemal, Orhan Karaca. Microsatellite-Based Insights into Bottleneck Dynamics in Awassi Sheep. KSU J. Agric Nat. 2026 Sep. 1;(Advanced Online Publication):242-56. doi:10.18016/ksutarimdoga.vi.1767827