Investigation of Cytotoxic, Colony-forming, and Anti-migratory Effects of Inula heterolepis Boiss. (Asteraceae) on Prostate Cancer

Kübra Uzun; Aslıhan Sena Köşker

doi:10.18016/ksutarimdoga.vi.1768714

TR EN

Inula heterolepis Boiss. (Asteraceae) Prostat Kanseri Üzerindeki Sitotoksik, Koloni Oluşturucu ve Anti-migratör Etkilerinin Araştırılması

Abstract

Bu çalışma Inula heterolepis'ten elde edilen farklı ekstre ve fraksiyonların sitotoksisitesini, koloni oluşumunu ve anti-migratör etkilerini araştırmayı amaçlamaktadır. Bu çalışmada ekstre ve fraksiyonların LNCaP ve HGF-1 hücrelerine karşı sitotoksik aktiviteleri MTT yöntemi ile araştırılmıştır. Ayrıca LNCaP hücreleri üzerinde 2 boyutlu koloni oluşumu ve in vitro hücre migrasyon deneyleri yapılmıştır. Sonuçlara göre kloroform fraksiyonu en aktif örnek olup, LNCaP hücrelerine karşı 12.91 ± 0,61 μg mL-1'lik bir IC50 değeriyle umut verici sitotoksik aktiviteye sahiptir. 10 μg mL-1'de değerlendirilen tüm ekstre ve fraksiyonlar 2 boyutlu koloni oluşumu deneyinde koloni oluşumunu azaltmıştır (p<.0001). Kloroform fraksiyonu ayrıca 24 ve 36. saatlerde hücre migrasyonunu da engellemiştir (p<.027). Sonuç olarak, n-butanol fraksiyonu hariç, test edilen tüm numuneler, LNCaP hücreleri üzerinde konsantrasyona bağlı olarak sitotoksik aktivite göstermiştir. Bu, Inula heterolepis'in LNCaP ve HGF-1 hücrelerine karşı ilk sitotoksik aktivite çalışmasıdır. Bu çalışma, Inula heterolepis ekstrelerinin prostat kanserine karşı daha ileri çalışmalar için mükemmel bir potansiyele sahip olduğunu göstermektedir.

Keywords

Project Number

TLO-224-13814

Investigation of Cytotoxic, Colony-forming, and Anti-migratory Effects of Inula heterolepis Boiss. (Asteraceae) on Prostate Cancer

Abstract

This study aimed to investigate the cytotoxicity, colony formation, and anti-migratory effects of different extracts and fractions derived from Inula heterolepis. In this study, the cytotoxic activities of the extracts against LNCaP and HGF-1 cells were investigated by the MTT method. It was also performed as a 2-dimensional colony formation and in vitro cell migration assay on LNCaP cells. According to the results, the chloroform fraction is the most active sample, and it has promising cytotoxic activity with an IC50 value of 12.91±0.61 μg mL-1 against the LNCaP cells. All extracts and fractions evaluated at 10 μg mL-1 decreased colony formation in the 2-dimensional colony formation experiment (p<.0001). The chloroform fraction also inhibited cell migration at 24 and 36 hours (p<.027). In conclusion, all the tested extracts and fractions, except for the n-butanol fraction, showed cytotoxic activity on LNCaP cells in a concentration-dependent manner. It is the first cytotoxic activity study of Inula heterolepis against LNCaP and HGF-1 cells. This observation suggests that Inula heterolepis extracts have excellent potential for further studies against prostate cancer.

Keywords

Supporting Institution

Erciyes University Scientific Research Projects

Project Number

TLO-224-13814

Ethical Statement

A decision from the ethics committee is not necessary for the work. We, the authors of this article, affirm that the data, information, and documents herein were acquired in adherence to academic and ethical standards, and that all information, documents, evaluations, and results are presented in compliance with scientific ethics and moral principles.

Thanks

The financial support from Erciyes University Scientific Research Projects (under project code TLO-2024-13814) is gratefully acknowledged. This study covers the undergraduate thesis at Erciyes University Faculty of Pharmacy. Also many thanks to Dr. Yalçın ERZURUMLU and Dr. İrem ÖNAL, who kindly gifted the cell lines.

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Kübra Uzun ^*
0000-0003-0381-5152
Türkiye

Aslıhan Sena Köşker
0009-0002-8540-6802
Türkiye

Early Pub Date

November 7, 2026

Publication Date

-

Submission Date

August 19, 2025

Acceptance Date

July 9, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1768714

IZ

https://izlik.org/JA39LB72ZJ

Cite

RIS / Bibtex

APA

Uzun, K., & Köşker, A. S. (2026). Investigation of Cytotoxic, Colony-forming, and Anti-migratory Effects of Inula heterolepis Boiss. (Asteraceae) on Prostate Cancer. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1768714

AMA

1.Uzun K, Köşker AS. Investigation of Cytotoxic, Colony-forming, and Anti-migratory Effects of Inula heterolepis Boiss. (Asteraceae) on Prostate Cancer. KSU J. Agric Nat. 2026;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1768714

Chicago

Uzun, Kübra, and Aslıhan Sena Köşker. 2026. “Investigation of Cytotoxic, Colony-Forming, and Anti-Migratory Effects of Inula Heterolepis Boiss. (Asteraceae) on Prostate Cancer”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1768714.

EndNote

Uzun K, Köşker AS (November 7, 2026) Investigation of Cytotoxic, Colony-forming, and Anti-migratory Effects of Inula heterolepis Boiss. (Asteraceae) on Prostate Cancer. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication

IEEE

[1]K. Uzun and A. S. Köşker, “Investigation of Cytotoxic, Colony-forming, and Anti-migratory Effects of Inula heterolepis Boiss. (Asteraceae) on Prostate Cancer”, KSU J. Agric Nat., no. Advanced Online Publication, Nov. 2026, doi: 10.18016/ksutarimdoga.vi.1768714.

ISNAD

Uzun, Kübra - Köşker, Aslıhan Sena. “Investigation of Cytotoxic, Colony-Forming, and Anti-Migratory Effects of Inula Heterolepis Boiss. (Asteraceae) on Prostate Cancer”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (November 7, 2026). https://doi.org/10.18016/ksutarimdoga.vi.1768714.

JAMA

1.Uzun K, Köşker AS. Investigation of Cytotoxic, Colony-forming, and Anti-migratory Effects of Inula heterolepis Boiss. (Asteraceae) on Prostate Cancer. KSU J. Agric Nat. 2026. doi:10.18016/ksutarimdoga.vi.1768714.

MLA

Uzun, Kübra, and Aslıhan Sena Köşker. “Investigation of Cytotoxic, Colony-Forming, and Anti-Migratory Effects of Inula Heterolepis Boiss. (Asteraceae) on Prostate Cancer”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication, Nov. 2026, doi:10.18016/ksutarimdoga.vi.1768714.

Vancouver

1.Kübra Uzun, Aslıhan Sena Köşker. Investigation of Cytotoxic, Colony-forming, and Anti-migratory Effects of Inula heterolepis Boiss. (Asteraceae) on Prostate Cancer. KSU J. Agric Nat. 2026 Nov. 7;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1768714