Investigation of Selected Biochemical Parameters in Buffaloes (Bubalus bubalis) Naturally Infected with Anaplasma marginale

Sezai Arslan; Mesut Şenel; Taraneh Oncel

doi:10.18016/ksutarimdoga.vi.1785278

TR EN

Anaplasma marginale ile Doğal Olarak Enfekte Olmuş Mandalarda (Bubalus bubalis) Seçilmiş Biyokimyasal Parametrelerin İncelenmesi

Öz

Bu çalışma, doğal olarak Anaplasma marginale ile enfekte olmuş su mandalarında serum biyokimyasal değişikliklerini değerlendirmek amacıyla yapılmıştır. Enfeksiyon, Giemsa ile boyanmış kan yaymalarında periferik intrasitoplazmik inklüzyon cisimciklerinin saptanmasıyla teşhis edilmiş ve rekabetçi ELISA (cELISA) testi ile doğrulanmıştır. Çalışmaya A. marginale pozitif bulunan 20 manda ile kontrol grubu olarak kullanılan klinik olarak sağlıklı 15 manda dahil edilmiştir. Her iki gruptan alınan serum örneklerinde albümin, total protein, total ve direkt bilirubin, alanin aminotransferaz (ALT), aspartat aminotransferaz (AST), alkalen fosfataz (ALP), gama-glutamil transferaz (GGT), amilaz (AMYL), kan üre azotu (BUN), kreatinin (CRE), glukoz, kalsiyum (Ca), fosfor (P), sodyum (Na), potasyum (K), klor (Cl), kreatin kinaz (CK), kreatin kinaz-MB izoenzimi (CK-MB), C-reaktif protein (CRP), prokalsitonin (PCT) ve yüksek duyarlılıklı kardiyak troponin I (hs-cTnI) parametreleri analiz edilmiştir. Klinik olarak enfekte mandalarda ateş, depresyon, iştahsızlık ve süt veriminde azalma gibi belirtiler gözlenmiştir. Kontrol grubuyla karşılaştırıldığında, enfekte hayvanlarda serum kalsiyum (p<0,001) ve glukoz (p<0,05) düzeyleri düşük, hs-cTnI düzeyleri yüksek (p<0,05) bulunmuştur. Diğer biyokimyasal parametreler açısından gruplar arasında istatistiksel olarak anlamlı bir farklılık saptanmamıştır. Bu bulgular, su mandalarında Anaplasma enfeksiyonunun genellikle hafif klinik semptomlarla seyretmesine rağmen hipokalsemi, hipoglisemi ve miyokardiyal hasara yol açabileceğini göstermektedir.

Anahtar Kelimeler

Investigation of Selected Biochemical Parameters in Buffaloes (Bubalus bubalis) Naturally Infected with Anaplasma marginale

Abstract

This study was conducted to evaluate the serum biochemical changes in water buffaloes naturally infected with Anaplasma marginale. The infection was diagnosed through the detection of peripheral intraerythrocytic inclusion bodies in Giemsa-stained blood smears, and confirmed using a competitive ELISA (cELISA) test. The study included 20 buffaloes that tested positive for A. marginale and 15 clinically healthy buffaloes as the control group. Serum samples from both infected and healthy animals were analyzed for the following biochemical parameters: albumin, total protein, total and direct bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), amylase (AMYL), blood urea nitrogen (BUN), creatinine (CRE), glucose, calcium (Ca), phosphorus (P), sodium (Na), potassium (K), chloride (Cl), creatine kinase (CK), creatine kinase-MB isoenzyme (CK-MB), C-reactive protein (CRP), procalcitonin (PCT), and high-sensitivity cardiac troponin I (hs-cTnI). Clinically, infected buffaloes showed signs such as fever, depression, loss of appetite, and reduced milk production. Compared to the control group, infected animals had significantly lower serum calcium (p<0.001) and glucose levels (p<0.05), along with higher hs-cTnI levels (p<0.05). No statistically significant differences were observed in the other biochemical parameters between the groups. These findings suggest that although Anaplasma infection in buffaloes often presents with mild clinical symptoms, it can still lead to hypocalcemia, hypoglycemia, and myocardial injury.

Keywords

References

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Details

Primary Language

English

Subjects

Veterinary Sciences (Other)

Journal Section

Research Article

Authors

Sezai Arslan ^*
0000-0001-9537-6024
Türkiye

Mesut Şenel
0000-0002-1736-9299
Türkiye

Taraneh Oncel
0000-0003-1039-5256
Türkiye

Early Pub Date

March 4, 2026

Publication Date

March 4, 2026

Submission Date

September 16, 2025

Acceptance Date

January 22, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1785278

IZ

https://izlik.org/JA35AX96RN

Cite

RIS / Bibtex

APA

Arslan, S., Şenel, M., & Oncel, T. (2026). Investigation of Selected Biochemical Parameters in Buffaloes (Bubalus bubalis) Naturally Infected with Anaplasma marginale. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication, 1119-1126. https://doi.org/10.18016/ksutarimdoga.vi.1785278