Determination of the Lytic Activity and Host Range of Bacteriophages Isolated Against Xanthomonas phaseoli pv. phaseoli and Xanthomonas citri pv. Fuscans

Büşran Sunyar; Mesude Figen Dönmez

doi:10.18016/ksutarimdoga.vi.1824634

TR EN

Xanthomonas phaseoli pv. phaseoli ve Xanthomonas citri pv. fuscans'a Karşı İzole Edilen Bakteriyofajların Litik Aktivitesinin ve Konakçı Aralığının Belirlenmesi

Öz

Bu çalışmada, fasulyede yaygın bakteriyel yanıklık hastalığının etkeni olan Xanthomonas phaseoli pv. phaseoli ve Xanthomonas citri pv. fuscans'a karşı bakteriyofajların in vitro biyokontrol potansiyeli araştırılmıştır. Çalışmada kullanılan 23 patojenik Xanthomonas strainlerinden dokuzu hastalıklı fasulye bitkilerinden yeni izole edilirken, geri kalan strainler laboratuvar kültür koleksiyonundan elde edilmiştir. Yağ asidi metil esteri (FAME) analizine dayanarak, yeni izole edilen strainler X. phaseoli pv. phaseoli ve X. citri pv. fuscans olarak tanımlanmıştır. Faj aracılı biyokontrol potansiyelini araştırmak amacıyla, Iğdır ilinden toplanan toprak ve atıksu örneklerinden toplam 23 bakteri strainine karşı faj izolasyonu yapılmıştır. Altı bakteriyofaj çift katmanlı agar yöntemi kullanılarak doğrulandı: üçü (Φ442-FUS, ΦMfb-50-FUS ve ΦMfb-53-FUS) Xpp strainlerine karşı etkiliyken, diğer üçü (Φ426-X, ΦMfb-17-X ve Mfb-54) Xpp ve Xcf strainlerine karşı aktivite gösterdi. Özellikle, ΦMfb-50, ΦMfb-53 ve ΦMfb-54 faj izolatları geniş konak aralıkları ve her iki patovara karşı da güçlü litik aktiviteler sergilerken, Φ426-X ve Φ442-FUS daha dar konak spektrumları gösterdi. Ek olarak, bazı faj izolatları Xanthomonas euvesicatoria ve Erwinia amylovora strainlerine karşı litik aktivite göstererek çapraz enfeksiyonlu biyolojik kontrol potansiyeline işaret etti. KVN-1 straini hariç, test edilen tüm Acidovorax citrulli strainleri faj izolatlarına dirençli bulunmuştur. Genel olarak, bulgular, çevre dostu ve hedefe özgü faj bazlı biyolojik kontrol stratejilerinin fasulyelerde yaygın görülen bakteriyel yanıklığın yönetimi için umut verici bir alternatif sunabileceğini vurgulamaktadır.

Anahtar Kelimeler

Determination of the Lytic Activity and Host Range of Bacteriophages Isolated Against Xanthomonas phaseoli pv. phaseoli and Xanthomonas citri pv. Fuscans

Abstract

In this study, the in vitro biocontrol potential of bacteriophages against Xanthomonas phaseoli pv. phaseoli and Xanthomonas citri pv. fuscans, the causal agents of common bacterial blight in common bean, was investigated. Among the 23 pathogenic Xanthomonas strains used in the study, nine were newly isolated from diseased bean plants, while the remaining strains were obtained from the laboratory culture collection. Based on fatty acid methyl ester (FAME) analysis, the newly isolated strains were identified as X. phaseoli pv. phaseoli and X. citri pv. fuscans. To explore phage-mediated biocontrol potential, phage isolations were performed from soil and wastewater samples collected from Iğdır Province against a total of 23 bacterial strains. Six bacteriophages were confirmed using the double-layer agar method: three (Φ442-FUS, ΦMfb-50-FUS, and ΦMfb-53-FUS) were effective against Xpp strains, while the other three (Φ426-X, ΦMfb-17-X, and ΦMfb-54-X) showed activity against Xcf strains. Notably, the ΦMfb-50, ΦMfb-53, and ΦMfb-54 phage isolates exhibited broad host ranges and strong lytic activities against both pathovars, whereas Φ426-X and Φ442-FUS displayed narrower host spectra. In addition, certain phage isolates demonstrated lytic activity against Xanthomonas euvesicatoria and Erwinia amylovora strains, indicating cross-infective biocontrol potential. Except for the KVN-1 strain, all Acidovorax citrulli strains tested were resistant to the phage isolates. Overall, the findings highlight that environmentally friendly and target-specific phage-based biocontrol strategies could provide a promising alternative for managing common bacterial blight in beans.

Keywords

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Details

Primary Language

English

Subjects

Phytopathology

Journal Section

Research Article

Authors

Büşran Sunyar ^*
0000-0001-8524-3308
Türkiye

Mesude Figen Dönmez
0000-0002-7992-8252
Türkiye

Early Pub Date

April 11, 2026

Publication Date

-

Submission Date

November 15, 2025

Acceptance Date

March 3, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1824634

IZ

https://izlik.org/JA22MF74GS

Cite

RIS / Bibtex

APA

Sunyar, B., & Dönmez, M. F. (2026). Determination of the Lytic Activity and Host Range of Bacteriophages Isolated Against Xanthomonas phaseoli pv. phaseoli and Xanthomonas citri pv. Fuscans. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication, 1441-1459. https://doi.org/10.18016/ksutarimdoga.vi.1824634