Isolation and Identification of Antagonistic and Plant Growth-Promoting Endophytic Bacteria for the Management of Pectobacterium betavasculorum in Sugar Beet

Şeval Koç; Murat Öztürk; Emin Aydin

doi:10.18016/ksutarimdoga.vi.1891433

EN TR

Isolation and Identification of Antagonistic and Plant Growth-Promoting Endophytic Bacteria for the Management of Pectobacterium betavasculorum in Sugar Beet

Abstract

This study aimed to isolate and identify endophytic bacteria from sugar beet (Beta vulgaris L.) grown in Yozgat province and to evaluate their biological control and plant growth-promoting (PGPB) potential against Pectobacterium betavasculorum, the causal agent of bacterial vascular necrosis and root rot. A total of 120 sugar beet tuber samples were collected, yielding 325 bacterial isolates. After preliminary screening, 221 isolates were retained. Pectolytic activity on potato, hypersensitive reaction on tobacco, and growth at 37 °C were tested to exclude potentially pathogenic isolates. Ultimately, 55 endophytic isolates were evaluated for antagonistic activity and PGP traits, including protease production, phosphate solubilization, indole-3-acetic acid (IAA) production, and ammonia production. Promising isolates were identified by MALDI-TOF MS. Among the isolates, 7 showed antagonistic activity against plant pathogens, 24 produced protease, 7 solubilized phosphate, 19 produced IAA, and 30 produced ammonia. Twelve promising isolates were identified, including Raoultella ornithinolytica, Bacillus thuringiensis israelensis, Paenibacillus polymyxa, and Serratia rubidaea. S. rubidaea SB 65.2 demonstrated the highest multifunctional potential. The results indicate that sugar beet harbors diverse endophytic bacteria with multiple biocontrol and PGP mechanisms, highlighting their ecological and functional diversity. Endophytic bacteria isolated from sugar beet represent promising candidates for sustainable management of P. betavasculorum and may contribute to environmentally friendly agricultural practices.

Keywords

Şeker Pancarında Pectobacterium betavasculorum ile Mücadelede Antagonistik ve Bitki Gelişimini Teşvik Edici Endofitik Bakterilerin İzolasyonu ve Tanılanması

Abstract

Bu çalışmada, Yozgat ilinde yetiştirilen şeker pancarından (Beta vulgaris L.) endofitik bakterilerin izolasyonu ve tanılanması ile bu izolatların şeker pancarında bakteriyel vasküler nekroz ve kök çürüklüğüne neden olan Pectobacterium betavasculorum’a karşı biyolojik kontrol ve bitki gelişimini teşvik edici (PGPB) potansiyellerinin belirlenmesi amaçlanmıştır. Farklı üretim alanlarından toplam 120 şeker pancarı yumru örneği toplanmış ve izolasyon çalışmaları sonucunda 325 bakteriyel izolat elde edilmiştir. Ön eleme sonrası 221 izolat değerlendirmeye alınmıştır. Olası patojen izolatları elemek amacıyla patates dilimlerinde pektolitik aktivite, tütünde hipersensitif reaksiyon ve 37 °C’de gelişim testleri uygulanmıştır. Değerlendirmeler sonucunda 55 endofitik izolat biyolojik kontrol ve PGP özellikleri bakımından incelenmiştir. Antagonistik etki, proteaz üretimi, fosfat çözünürlüğü, indol-3-asetik asit (IAA) üretimi ve amonyak üretimi test edilmiştir. Ümitvar izolatlar MALDI-TOF MS tekniği ile tanılanmıştır. İzolatların 7’si antagonistik aktivite göstermiş, 24’ü proteaz üretmiş, 7’si fosfat çözmüş, 19’u IAA üretmiş ve 30’u amonyak üretmiştir. Fonksiyonel özellikleri bakımından öne çıkan 12 izolat Raoultella ornithinolytica, Bacillus thuringiensis israelensis, Paenibacillus polymyxa ve Serratia rubidaea gibi türler olarak tanılanmıştır. S. rubidaea SB 65.2 izolatı çoklu etki mekanizmaları ile en yüksek potansiyeli göstermiştir. Elde edilen bulgular, şeker pancarı endofitik bakterilerinin zengin biyolojik çeşitliliğe ve çok yönlü biyokontrol mekanizmalarına sahip olduğunu ortaya koymaktadır. Şeker pancarından izole edilen endofitik bakteriler, P. betavasculorum’un sürdürülebilir yönetiminde umut verici biyolojik ajanlar olarak değerlendirilebilir ve çevre dostu tarımsal uygulamalara katkı sağlayabilir.

Keywords

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Details

Primary Language

English

Subjects

Phytopathology

Journal Section

Research Article

Authors

Şeval Koç
0009-0005-5892-0517
Türkiye

Murat Öztürk ^*
0000-0002-9677-3651
Türkiye

Emin Aydin
0000-0002-7853-8855
Türkiye

Early Pub Date

October 23, 2026

Publication Date

-

Submission Date

February 17, 2026

Acceptance Date

April 29, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1891433

IZ

https://izlik.org/JA62ND78NN

Cite

RIS / Bibtex

APA

Koç, Ş., Öztürk, M., & Aydin, E. (2026). Isolation and Identification of Antagonistic and Plant Growth-Promoting Endophytic Bacteria for the Management of Pectobacterium betavasculorum in Sugar Beet. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1891433

AMA

1.Koç Ş, Öztürk M, Aydin E. Isolation and Identification of Antagonistic and Plant Growth-Promoting Endophytic Bacteria for the Management of Pectobacterium betavasculorum in Sugar Beet. KSU J. Agric Nat. 2026;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1891433

Chicago

Koç, Şeval, Murat Öztürk, and Emin Aydin. 2026. “Isolation and Identification of Antagonistic and Plant Growth-Promoting Endophytic Bacteria for the Management of Pectobacterium Betavasculorum in Sugar Beet”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1891433.

EndNote

Koç Ş, Öztürk M, Aydin E (October 1, 2026) Isolation and Identification of Antagonistic and Plant Growth-Promoting Endophytic Bacteria for the Management of Pectobacterium betavasculorum in Sugar Beet. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication

IEEE

[1]Ş. Koç, M. Öztürk, and E. Aydin, “Isolation and Identification of Antagonistic and Plant Growth-Promoting Endophytic Bacteria for the Management of Pectobacterium betavasculorum in Sugar Beet”, KSU J. Agric Nat., no. Advanced Online Publication, Oct. 2026, doi: 10.18016/ksutarimdoga.vi.1891433.

ISNAD

Koç, Şeval - Öztürk, Murat - Aydin, Emin. “Isolation and Identification of Antagonistic and Plant Growth-Promoting Endophytic Bacteria for the Management of Pectobacterium Betavasculorum in Sugar Beet”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (October 1, 2026). https://doi.org/10.18016/ksutarimdoga.vi.1891433.

JAMA

1.Koç Ş, Öztürk M, Aydin E. Isolation and Identification of Antagonistic and Plant Growth-Promoting Endophytic Bacteria for the Management of Pectobacterium betavasculorum in Sugar Beet. KSU J. Agric Nat. 2026. doi:10.18016/ksutarimdoga.vi.1891433.

MLA

Koç, Şeval, et al. “Isolation and Identification of Antagonistic and Plant Growth-Promoting Endophytic Bacteria for the Management of Pectobacterium Betavasculorum in Sugar Beet”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication, Oct. 2026, doi:10.18016/ksutarimdoga.vi.1891433.

Vancouver

1.Şeval Koç, Murat Öztürk, Emin Aydin. Isolation and Identification of Antagonistic and Plant Growth-Promoting Endophytic Bacteria for the Management of Pectobacterium betavasculorum in Sugar Beet. KSU J. Agric Nat. 2026 Oct. 1;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1891433