In vitro Micropropagation of Anacamptis laxiflora Growing Naturally in Elazığ Region

Dilek Nur Ekinoğlu; Tuba Türkoğlu

doi:10.18016/ksutarimdoga.vi.1914943

EN TR

In vitro Micropropagation of Anacamptis laxiflora Growing Naturally in Elazığ Region

Abstract

In this study, it was aimed to obtain tubers in vitro by using shoots obtained from the tubers of the Anacamptis laxiflora plant, which is under threat of extinction due to unconscious collection for salep production and whose micropropagation is intended, as an explant source. Murashige Skoog (MS) nutrient medium was used to obtain shoots from tubers. Shoots were obtained 45 days after the tubers were planted in MS nutrient medium after being sterilized by applying different concentrations of surface sterilizers for different periods of time and these shoots were observed for tuber formation by culturing in MS nutrient media containing two different concentrations of Benzyl Amino Purine (BAP) (0.5 mg L-1 and 1 mg L-1) and different concentrations and combinations of Naphthalene Acetic Acid (NAA), Picloram (P), Indole Acetic Acid (IAA), Indole Butyric Acid (IBA) and Dichlorophenoxy acetic acid (2,4D) plant growth regulators. As a result, a lump began to form after 30 days. The most effective concentration in terms of tuber formation was achieved with the combined use of 0.5 mg L-1 BAP and 0.5 mg L-1 NAA plant growth regulators, while the least effective plant growth regulators were 2,4D and IBA. The most important aspect that distinguishes this study from the limited number of micropropagation studies carried out with salep tubers in the literature is the use of shoots obtained from the tubers as an explant source and the observation of successful tuber regeneration as a result.

Keywords

Ethical Statement

The experiments conducted within the scope of this research were based on plant tissue culture, and no applications were made on humans or animals, and no human-derived biological material was used.

Thanks

This study was derived from Dilek Nur EKİNOĞLU’s master’s thesis. It was supported by Fırat University Scientific Research Projects Management Unit. I respectfully commemorate my advisor, the late Assoc. Prof. Dr. Nazmi GÜR, who provided me with invaluable assistance throughout the process of preparing this study

Elazığ Bölgesinde Doğal Olarak Yetişen Anacamptis laxiflora’nın İn vitro Mikroçoğaltımı

Abstract

Bu çalışmada, salep üretimi için doğadan bilinçsizce toplanması nedeniyle nesli tükenme tehlikesi altında olan ve mikroçoğaltılması amaçlanan Anacamptis laxiflora bitkisinin yumrularından elde edilen sürgünler kullanılarak in vitro yumru elde edilmesi amaçlanmıştır. Yumrulardan sürgün elde etmek için Murashige Skoog (MS) besin ortamı kullanılmıştır. Yumrular, farklı konsantrasyonlardaki yüzey sterilizatörlerinin farklı sürelerde uygulanması ile sterilize edildikten sonra MS besin ortamına ekilmiş ve 45 gün sonra sürgünler elde edilmiştir. Oluşan bu sürgünler, Benzil Amino Purin (BAP)’in iki farklı konsantrasyonu (0,5 mg L-1 ve 1 mg L-1) ile farklı konsantrasyon ve kombinasyonlarda Naftalen Asetik Asit (NAA), Pikloram (P), İndol Asetik Asit (IAA), İndol Bütirik Asit (IBA) ve Diklorofenoksi asetik asit (2,4D) bitki büyüme düzenleyicileri içeren MS besin ortamında kültüre alınarak yumru oluşumu takip edilmiştir. Sonuç olarak, 30 gün sonra yumru gelişmeye başlamıştır. Yumru oluşumu açısından en etkili konsantrasyon, 0,5 mg L-1 BAP ve 0,5 mg L-1 NAA bitki büyüme düzenleyicilerinin birlikte kullanımıyla elde edilirken, en az etkili bitki büyüme düzenleyicileri 2,4D ve IBA olmuştur. Bu çalışmayı literatürde salep yumrularıyla yapılan sınırlı sayıdaki mikroçoğaltım çalışmalarından ayıran en önemli özellik, yumrulardan elde edilen sürgünlerin eksplant kaynağı olarak kullanılması ve bunun sonucunda başarılı yumru rejenerasyonunun gözlemlenmesidir.

Keywords

Ethical Statement

Bu araştırma kapsamında yürütülen deneyler bitki doku kültürü temelli olup, insan veya hayvanlar üzerinde herhangi bir uygulama yapılmamış ve insan kaynaklı biyolojik materyal kullanılmamıştır

Thanks

Bu çalışma, Dilek Nur EKİNOĞLU'nun yüksek lisans tezinden türetilmiştir. Fırat Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi tarafından desteklenmiştir. Bu çalışmanın hazırlanması sürecinde bana paha biçilmez yardımlarda bulunan danışmanım, merhum Doç. Dr. Nazmi GÜR'ü saygıyla anıyorum.

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Details

Primary Language

English

Subjects

Plant Biotechnology

Journal Section

Research Article

Authors

Dilek Nur Ekinoğlu ^*
0000-0002-6311-7228
Türkiye

Tuba Türkoğlu
0000-0001-6481-6218
Türkiye

Early Pub Date

November 7, 2026

Publication Date

-

Submission Date

March 24, 2026

Acceptance Date

June 29, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1914943

IZ

https://izlik.org/JA25GA62NB

Cite

RIS / Bibtex

APA

Ekinoğlu, D. N., & Türkoğlu, T. (2026). In vitro Micropropagation of Anacamptis laxiflora Growing Naturally in Elazığ Region. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1914943

AMA

1.Ekinoğlu DN, Türkoğlu T. In vitro Micropropagation of Anacamptis laxiflora Growing Naturally in Elazığ Region. KSU J. Agric Nat. 2026;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1914943

Chicago

Ekinoğlu, Dilek Nur, and Tuba Türkoğlu. 2026. “In Vitro Micropropagation of Anacamptis Laxiflora Growing Naturally in Elazığ Region”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1914943.

EndNote

Ekinoğlu DN, Türkoğlu T (November 7, 2026) In vitro Micropropagation of Anacamptis laxiflora Growing Naturally in Elazığ Region. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication

IEEE

[1]D. N. Ekinoğlu and T. Türkoğlu, “In vitro Micropropagation of Anacamptis laxiflora Growing Naturally in Elazığ Region”, KSU J. Agric Nat., no. Advanced Online Publication, Nov. 2026, doi: 10.18016/ksutarimdoga.vi.1914943.

ISNAD

Ekinoğlu, Dilek Nur - Türkoğlu, Tuba. “In Vitro Micropropagation of Anacamptis Laxiflora Growing Naturally in Elazığ Region”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (November 7, 2026). https://doi.org/10.18016/ksutarimdoga.vi.1914943.

JAMA

1.Ekinoğlu DN, Türkoğlu T. In vitro Micropropagation of Anacamptis laxiflora Growing Naturally in Elazığ Region. KSU J. Agric Nat. 2026. doi:10.18016/ksutarimdoga.vi.1914943.

MLA

Ekinoğlu, Dilek Nur, and Tuba Türkoğlu. “In Vitro Micropropagation of Anacamptis Laxiflora Growing Naturally in Elazığ Region”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication, Nov. 2026, doi:10.18016/ksutarimdoga.vi.1914943.

Vancouver

1.Dilek Nur Ekinoğlu, Tuba Türkoğlu. In vitro Micropropagation of Anacamptis laxiflora Growing Naturally in Elazığ Region. KSU J. Agric Nat. 2026 Nov. 7;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1914943