Chemical Composition of Laurel, Cypress, and Eucalyptus Essential Oils and Their Larvicidal and Ovulation-Inhibiting Effects Against Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)

Murat Öztürk; Muhammet Mustafa Akıner; Kübra Demirtaş; Fatih Şaban Beriş; Hilal Bedir

doi:10.18016/ksutarimdoga.vi.1949740

TR EN

Defne, Selvi ve Okaliptüs Esansiyel Yağlarının Kimyasal Kompozisyonu ve Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)'e Karşı Larva Öldürücü ve Yumurtlamayı Engelleyici Etkileri

Abstract

Aedes albopictus (Skuse, 1894) (Diptera: Culicidae), dünya çapında çeşitli arbovirüslerin taşınmasından sorumlu, oldukça istilacı bir vektördür. Bu çalışma, Türkiye'nin Doğu Karadeniz bölgesinden toplanan Laurus nobilis L. (Lauraceae), Cupressus macrocarpa Hartw. (Cupressaceae) ve Eucalyptus globulus Labill. (Myrtaceae)'den elde edilen uçucu yağların kimyasal bileşimini, Aedes albopictus'a karşı larva öldürücü aktivitesini ve yumurtlamayı engelleyici etkilerini değerlendirmeyi amaçlamıştır. Uçucu yağlar hidroddistillasyon yöntemiyle elde edilmiş ve kimyasal bileşenleri GC-MS kullanılarak tanımlanmıştır. Esansiyel yağ çözeltileri, %96 etanol kullanılarak 1:1, 1:8, 1:16, 1:32 ve 1:64 ; v:v konsantrasyonlarında hazırlandı. Öldürücü konsantrasyonları belirlemek için larva öldürücü biyolojik testleri yapılmış ve yumurtlama tercihi, birden fazla konsantrasyonda değerlendirilerek yumurtlamayı engelleyici indeksi hesaplanmıştır. GC-MS analizi, Laurus nobilis için ana bileşenlerin p-simen (%31,81) ve ökaliptol (%15,88), Cupressus macrocarpa için sabinen (%27,25) ve terpinen-4-ol (%25,01) ve Eucalyptus globulus için ökaliptol (%39,09) olduğunu ortaya koymuştur. Larva öldürücü testlerde, Laurus nobilis 53,19 ppm'lik LC50 değeriyle en yüksek toksisiteyi göstermiş, bunu Cupressus macrocarpa (58,42 ppm) ve Eucalyptus globulus (62,15 ppm) izlemiştir. Ayrıca, tüm uçucu yağlar en yüksek yüzey konsantrasyonunda -0,95 ile -1,00 arasında değişen yumurtlamayı engelleyici etki değerleriyle olağanüstü yumurtlamayı engelleyici etki göstermiştir. Laurus nobilis düşük dozlarda tutarlı bir engelleyicilik gösterirken, Cupressus macrocarpa iki fazlı bir tepki gösterdi; 14,9 ppm'de hafif bir çekici etki gösterdikten sonra daha yüksek konsantrasyonlarda güçlü bir engelleyici haline gelmiştir. Doğu Karadeniz florasından, özellikle Laurus nobilis'ten elde edilen uçucu yağlar, Aedes albopictus popülasyonlarıyla mücadele için çevre dostu larva öldürücü ve davranış yönlendiriciler olarak önemli bir potansiyele sahiptir.

Keywords

Ethical Statement

Çalışmanın tüm süreçlerinin araştırma ve yayın etiğine uygun olduğunu, etik kurallara ve bilimsel atıf gösterme ilkelerine uyduğumu beyan ederim.

Chemical Composition of Laurel, Cypress, and Eucalyptus Essential Oils and Their Larvicidal and Ovulation-Inhibiting Effects Against Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)

Abstract

Aims: Aedes albopictus (Skuse, 1895) (Diptera: Culicidae) is a highly invasive vector responsible for the transmission of several arboviruses worldwide. This study aimed to evaluate the chemical composition, larvicidal activity, and oviposition deterrent effects of EOs extracted from Laurus nobilis L. (Lauraceae), Cupressus macrocarpa Hartw. (Cupressaceae), and Eucalyptus globulus Labill. (Myrtaceae) harvested from the Eastern Black Sea region of Türkiye against Ae. albopictus. Materials and Methods: Essential oils were obtained by hydrodistillation and their chemical constituents were identified using GC-MS. Larvicidal bioassays were conducted to determine the median lethal concentrations LC50, and oviposition preference was evaluated across multiple concentrations to calculate the Oviposition Deterrence Index (ODI). Results: GC-MS analysis revealed that the major constituents were p-cymene (%31.81) and eucalyptol (%15.88) for L. nobilis, sabinene (%27.25) and terpinen-4-ol (%25.01) for C. macrocarpa, and eucalyptol (%39.09) for E. globulus. In larvicidal assays, L. nobilis exhibited the highest toxicity with an LC50 of 53.19 ppm, followed by C. macrocarpa (58.42 ppm) and E. globulus (62.15 ppm). Furthermore, all EOs demonstrated exceptional oviposition deterrence at the highest surface concentration (1.2738 µL/cm²), with ODI values ranging from -0.95 to -1.00. While L. nobilis maintained consistent deterrence at lower doses, C. macrocarpa showed a biphasic response, acting as a slight attractant at 14.9 ppm before becoming a potent deterrent at higher concentrations. Discussion: Our findings suggest that EOs from the Eastern Black Sea flora, particularly L. nobilis, possess significant potential as eco-friendly larvicides and behavioral modifiers for the management of Ae. albopictus populations.

Keywords

Ethical Statement

I declare that all processes of this study comply with research and publication ethics, and that I have adhered to ethical rules and principles of scientific citation.

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Details

Primary Language

English

Subjects

Entomology, Animal Behaviour, Animal Developmental and Reproductive Biology, Invertebrate Biology, Medicinal and Aromatic Plants

Journal Section

Research Article

Authors

Murat Öztürk ^*
0000-0001-7022-5158
Türkiye

Muhammet Mustafa Akıner
0000-0002-7658-3236
Türkiye

Kübra Demirtaş
0009-0004-0441-7492
Türkiye

Fatih Şaban Beriş
0000-0002-0535-943X
Türkiye

Hilal Bedir
0000-0002-6260-9132
Türkiye

Early Pub Date

October 23, 2026

Publication Date

-

Submission Date

May 12, 2026

Acceptance Date

June 26, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1949740

IZ

https://izlik.org/JA35YX94ZS

Cite

RIS / Bibtex

APA

Öztürk, M., Akıner, M. M., Demirtaş, K., Beriş, F. Ş., & Bedir, H. (2026). Chemical Composition of Laurel, Cypress, and Eucalyptus Essential Oils and Their Larvicidal and Ovulation-Inhibiting Effects Against Aedes albopictus (Skuse, 1894) (Diptera: Culicidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1949740

AMA

1.Öztürk M, Akıner MM, Demirtaş K, Beriş FŞ, Bedir H. Chemical Composition of Laurel, Cypress, and Eucalyptus Essential Oils and Their Larvicidal and Ovulation-Inhibiting Effects Against Aedes albopictus (Skuse, 1894) (Diptera: Culicidae). KSU J. Agric Nat. 2026;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1949740

Chicago

Öztürk, Murat, Muhammet Mustafa Akıner, Kübra Demirtaş, Fatih Şaban Beriş, and Hilal Bedir. 2026. “Chemical Composition of Laurel, Cypress, and Eucalyptus Essential Oils and Their Larvicidal and Ovulation-Inhibiting Effects Against Aedes Albopictus (Skuse, 1894) (Diptera: Culicidae)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1949740.

EndNote

Öztürk M, Akıner MM, Demirtaş K, Beriş FŞ, Bedir H (October 1, 2026) Chemical Composition of Laurel, Cypress, and Eucalyptus Essential Oils and Their Larvicidal and Ovulation-Inhibiting Effects Against Aedes albopictus (Skuse, 1894) (Diptera: Culicidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication

IEEE

[1]M. Öztürk, M. M. Akıner, K. Demirtaş, F. Ş. Beriş, and H. Bedir, “Chemical Composition of Laurel, Cypress, and Eucalyptus Essential Oils and Their Larvicidal and Ovulation-Inhibiting Effects Against Aedes albopictus (Skuse, 1894) (Diptera: Culicidae)”, KSU J. Agric Nat., no. Advanced Online Publication, Oct. 2026, doi: 10.18016/ksutarimdoga.vi.1949740.

ISNAD

Öztürk, Murat - Akıner, Muhammet Mustafa - Demirtaş, Kübra - Beriş, Fatih Şaban - Bedir, Hilal. “Chemical Composition of Laurel, Cypress, and Eucalyptus Essential Oils and Their Larvicidal and Ovulation-Inhibiting Effects Against Aedes Albopictus (Skuse, 1894) (Diptera: Culicidae)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (October 1, 2026). https://doi.org/10.18016/ksutarimdoga.vi.1949740.

JAMA

1.Öztürk M, Akıner MM, Demirtaş K, Beriş FŞ, Bedir H. Chemical Composition of Laurel, Cypress, and Eucalyptus Essential Oils and Their Larvicidal and Ovulation-Inhibiting Effects Against Aedes albopictus (Skuse, 1894) (Diptera: Culicidae). KSU J. Agric Nat. 2026. doi:10.18016/ksutarimdoga.vi.1949740.

MLA

Öztürk, Murat, et al. “Chemical Composition of Laurel, Cypress, and Eucalyptus Essential Oils and Their Larvicidal and Ovulation-Inhibiting Effects Against Aedes Albopictus (Skuse, 1894) (Diptera: Culicidae)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication, Oct. 2026, doi:10.18016/ksutarimdoga.vi.1949740.

Vancouver

1.Murat Öztürk, Muhammet Mustafa Akıner, Kübra Demirtaş, Fatih Şaban Beriş, Hilal Bedir. Chemical Composition of Laurel, Cypress, and Eucalyptus Essential Oils and Their Larvicidal and Ovulation-Inhibiting Effects Against Aedes albopictus (Skuse, 1894) (Diptera: Culicidae). KSU J. Agric Nat. 2026 Oct. 1;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1949740