Bazı Schiff Bazlarının Saccharomyces cerevisiae BY4741 Kültür Ortamlarında Biyokimyasal Parametreler Üzerine Etkileri

Ayşe Dilek Özşahin; Nesrin Bozhan

TR EN

Bazı Schiff Bazlarının Saccharomyces cerevisiae BY4741 Kültür Ortamlarında Biyokimyasal Parametreler Üzerine Etkileri

Öz

Yeni sentezlenmiş bazı Schiff bazlarının kullanılmadan önce canlılar üzerindeki biyokimyasal etkilerinin incelenmesi gerekir. Bu amaçla Saccharomyces cerevisiae en önemli hücre modeli içinde yer alır. S. cerevisiae’daki metabolik özellikler, yüksek yapılı organizmalara benzerlik gösterdiği için elde edilen sonuçlar da paralellik göstermektedir.

Çalışmada; yeni sentezlenmiş Schiff bazlarının S. cerevisiae BY4741 kültür ortamlarında lipit peroksidasyon (MDA), total protein ve glutatyon analizleri yapılmıştır. Bu amaçla; deneyde kullanılan S. cerevisiae BY4741’in gelişimi ve çoğalması için YEDP besiyeri ortamı hazırlandı. Uygulama grupları için; schiff bazların her birinden 2ppm, 4 ppm ve 8 ppm olacak şekilde kültür ortamına ilave edildi. Elde edilen süpernatant ile; GSH,total protein ve MDA analizleri yapıldı.

Lipid peroksidayon sonuçları kontrol grubu ile karşılaştırıldığında schiff bazı gruplarının lipid peroksidasyon önleme etkilerinin olmadığı belirlenirken, yeni sentezlenen schiff bazlarının kontrol grubuna göre glutatyon ve total protein miktarlarının oldukça belirgin düzeyde yüksek olduğu saptandı (p<0.0001).

Sonuç olarak; yeni sentezlemiş Schiff bazlarının S. cerevisiae’nın biyokimyasal ve savunma sistemi üzerinde farklı etkilere sahip olduğu gözlemlendi. Özellikle antioksidan savunma sistemi üzerinde açığa çıkan sonuçların diğer canlı modelleri üzerindeki benzer çalışmalara kaynak olacağı ve in vivo sistemler kullanılarak yapılacak ileriki çalışmalara destek olarak, literatür bilgisine katkıda bulunulacağı düşünülmektedir.

Anahtar Kelimeler

Schiff bazı,Saccharomyces cerevisiae,MDA,GSH,Toplam Protein

The Effects of Some Schiff Bases on Biochemical Parameters in Saccharomyces cerevisiae BY4741 Cultural Environments

Abstract

Biochemical effects of newly synthesized some Schiff bases on living creatures need to be investigated before using them. For this purpose, Saccharomyces cerevisiae is involved in the most important cell model. Because metabolic properties of S. cerevisiae are similar to the highly organized organisms, the results show parallelism, as well.

In the present study; lipid peroxidation (MDA), total protein, and glutathione analyses of newly synthesized Schiff bases were conducted in S. cerevisiae BY4741 culture media. For this purpose, YEDP medium was prepared for development and growth of S. cerevisiae BY4741 used in the experiment. 2ppm, 4 ppm, and 8 ppm from each of Schiff bases were added in to culture medium for application groups. GSH, total protein and MDA analyses were carried out with the obtained supernatant.

When the results of lipid peroxidation were compared with the control group, it was determined that the schiff base groups had no lipid peroxidation inhibition effects, and the newly synthesized schiff bases were found to have significantly higher amounts of glutathione and total protein than the control group (p<0.0001).

As a result, newly synthesized Schiff bases were determined to have different effects on biochemical and defense system of S. cerevisiae. In particular, the results of the antioxidant defense system will be a source for similar studies on other living models and will contribute to the knowledge of the literature as support for future studies using in vivo systems.

The data obtained was then evaluated in ThermaCAM software program. The results show that thermal camera imaging technique offers prospects as a practical method for assessing the aerobic stability of silages on farm.

Keywords

Schiff base,Saccharomyces cerevisiae,MDA,GSH,Total Protein

References

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Ayşe Dilek Özşahin ^*

Nesrin Bozhan

Publication Date

April 30, 2018

Submission Date

January 20, 2017

Acceptance Date

April 27, 2017

Published in Issue

Year 2018 Volume: 21 Number: 2

IZ

https://izlik.org/JA73UW56GL

Cite

RIS / Bibtex

APA

Özşahin, A. D., & Bozhan, N. (2018). Bazı Schiff Bazlarının Saccharomyces cerevisiae BY4741 Kültür Ortamlarında Biyokimyasal Parametreler Üzerine Etkileri. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 21(2), 131-140. https://izlik.org/JA73UW56GL