Astragalus diphtherites FENZL var. diphtherites ve Astragalus gymnalopecias RECH. FIL’in Gövde ve Kök Kısımlarından Farklı Çözücüler ile Elde Edilen Özütlerin İnvitro Antioksidan ve Antimikrobiyal Özelliklerinin Belirlenmesi

Cumali Keskin; Hasan Çetin Özen; Zuhal Toker; Göksel Kızıl; Murat Kızıl

doi:10.18016/ksudobil.322478

EN TR

Determination of in vitro antioxidant and antimicrobial properties of shoot and root extracts of Astragalus diphtherites FENZL var. diphtherites and Astragalus gymnalopecias RECH. FIL. obtained by different solvents.

Abstract

The aim of this study is to investigate the antioxidant and antimicrobial properties of shoot and root parts of Astragalus diphtherites var. diphtherites (A. diphtherites) and Astragalus gymnalopecias (A. gymnalopecias) species which were subjected to solvent series having different polarity. Total phenolic, total flavonoid, 2.2-diphenyl-1-picryl-hydrazyl (DPPH), metal chelating, reducing power and hydroxy radicals scavenging activities of the extracts were tested in order to determine antioxidant properties. While the highest amount of total phenolic contents was obtained from the methanol extract in the shoot part of A. diphtherites and from the acetone extract in the root part of it, it was obtained from the methanol extract in the shoot part of A. gymnalopecias and from the ethyl acetate extract in the root part of it. The highest amount of total flavonoids and reducing power activities of A. diphtherites and A. gymnalopecias were obtained from the acetone extracts in the shoot part and from the ethyl acetate extracts in the root part. While the highest DPPH radical scavenging activity was determined in the methanol extract in the shoot part of A. diphtherites and in the acetone extract in the root part of it, it was determined in the methanol extract in the shoot part of A. gymnalopecias and in the ethyl acetate extract in the root part of it. The highest metal chelating activity was seen in the methanol extracts that were obtained from shoot parts of the A. diphtherites, and in the hexane extracts that were obtained from of the root part of it, whereas it was seen in the methanol extracts that were obtained from the shoot and root part of the A. gymnalopecias. The ethyl acetate extracts of the shoot and root part in both species showed the highest hydroxyl radical scavenging activity. It was determined that acetone and methanol extracts of the shoot part of A. gymnalopecias have inhibition effect on the growth of Streptococcus pyogenes

Keywords

Astragalus diphtherites FENZL var. diphtherites,Astragalus gymnalopecias RECH. FIL.,Antioxidant,Antimicrobial,Biological activity

Astragalus diphtherites FENZL var. diphtherites ve Astragalus gymnalopecias RECH. FIL’in Gövde ve Kök Kısımlarından Farklı Çözücüler ile Elde Edilen Özütlerin İnvitro Antioksidan ve Antimikrobiyal Özelliklerinin Belirlenmesi

Öz

Bu çalışmanın amacı farkı polariteye sahip çözücü serilerinden geçiren Astragalus diphtherites var. diphtherites (A. diphtherites) ve Astragalus gymnalopecias (A. gymnalopecias) türlerinin gövde ve kök kısımlarının antioksidan ve antimikrobiyal özelliklerinin araştırılmasıdır. Antioksidan özellikleri belirlemek için özütlerin total fenolik, total flavonoid, DPPH, metal şelatlama, indirgeme gücü ve hidroksi radikalini söndürme aktiviteleri test edildi. En yüksek total fenolik bileşen miktarı A. diphtherites’ de gövdede metanol, kökte ise aseton özütünden elde edilirken A. gymnalopecias’ ta gövdede metanol kökte ise etil asetat özütünde tespit edildi. A. diphtherites ve A. gymnalopecias’ ta en yüksek total flavonoid miktarları ve indirgeme gücü aktiviteleri gövdede aseton kökte ise etil asetat özütlerinden elde edildi. A. diphtherites için en yüksek DPPH radikalini söndürme aktivitesi, gövdede metanol kökte ise aseton özütünde, A. gymnalopecias’ ta gövdede metanol kökte ise etil asetat özütünde tespit edildi. A. diphtherites’ te gövde kısmından elde edilen metanol ve kök kısmından elde edilen hekzan özütleri, A. gymnalopecias’ ta ise gövde ve kök kısımlarının metanol özütleri en yüksek metal şelatlama aktivitesi gösterdi. Her iki türde de gövde ve kök etil asetat özütleri en yüksek hidroksil radikali söndürme aktivitesi gösterdi. A. gymnalopecias’ın gövde aseton ve metanol özütlerinin Streptococcus pyogenes’in büyümesi üzerinde inhibisyon etkisine sahip olduğu tespit edildi

Anahtar Kelimeler

Astragalus diphtherites FENZL var. diphtherites,Astragalus gymnalopecias RECH. FIL.,Antioksidan,Antimikrobiyal,Biyolojik aktivite

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Cumali Keskin ^*
0000-0003-3758-0654

Hasan Çetin Özen
0000-0001-6670-6469

Zuhal Toker
0000-0003-1038-9091

Göksel Kızıl
0000-0001-9603-0631

Murat Kızıl
0000-0003-2648-4915

Publication Date

April 30, 2018

Submission Date

June 19, 2017

Acceptance Date

August 27, 2017

Published in Issue

Year 2018 Volume: 21 Number: 2

DOI

https://doi.org/10.18016/ksudobil.322478

IZ

https://izlik.org/JA29MK32BN

Cite

RIS / Bibtex

APA

Keskin, C., Özen, H. Ç., Toker, Z., Kızıl, G., & Kızıl, M. (2018). Astragalus diphtherites FENZL var. diphtherites ve Astragalus gymnalopecias RECH. FIL’in Gövde ve Kök Kısımlarından Farklı Çözücüler ile Elde Edilen Özütlerin İnvitro Antioksidan ve Antimikrobiyal Özelliklerinin Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 21(2), 157-166. https://doi.org/10.18016/ksudobil.322478

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