Oil Hydrocarbon Degradation Capability of Bacterial Strains Isolated from the Sapanca Lake, Turkey

Pelin S Çiftçi Türetken; Gülşen Altuğ; Mine Çardak; Kemal Güneş

doi:10.18016/ksudobil.344593

EN TR

Oil Hydrocarbon Degradation Capability of Bacterial Strains Isolated from the Sapanca Lake, Turkey

Abstract

In this study, the analyses were carried out to determine oil-degradation capacity and oil-resistance levels of bacteria isolated from water surface (0-30 cm) of nine stations of the Sapanca Lake from September of 2008 to May of 2010. Twenty-seven bacteria species belonging three classes and six families total of eighty-five wild bacteria were identified and screened against crude oil with respect to Minimum Inhibitory Concentration (MIC). Bacterial isolates showing resistance against crude oil were chosen for Emulsification Index (E₂₄) test. Isolates displaying higher E₂₄ values were selected for further degradation tests regarding pH and oil thickness values in experimental setups. Fifty bacterial strains of eighty-five isolates were recorded to be resistant against oil hydrocarbon. Positive reactions percentages of the isolates against crude oil were detected in variable ranges between 25% and 100%. The results of the emulsification index test for crude oil indicated that the isolates of S49-Stenotrophomonas maltophila, S50-Aeromonas hydrophila, S59-E. coli1, S38-Aeromonas hydrophila and S43-Enterobacter cloaceae have higher emulsification percentages. The results of the degradation test showed that isolate of S59-E. coli1 has higher degradation ability than all isolates tested. The bacteria screened against crude oil were detected to be sensitive against crude oil during the first year of the study. Presence of petroleum-resistant bacteria in subsequent tests was associated with the unexpected oil spill occurred in the Sapanca Lake at the time. Detected crude oil resistant bacteria isolates were stocked for a possible use in upcoming bioremediation related studies.

Keywords

Crude oil,wild bacterial isolates,minimum inhibitory concentration,Sapanca Lake

Sapanca Gölü’nden İzole Edilen Bakteri Suşlarının Petrol Hidrokarbonlarını Parçalama Yetenekleri

Öz

Bu çalışmada, Eylül 2008 ile Mayıs 2010 tarihleri arasında Sapanca Gölü’nde belirlenen dokuz istasyonda yüzey sularından (0-30 cm) izole edilen bakterilerin petrol parçalama kapasiteleri ve direnç düzeylerini belirlemek amacıyla analizler yapılmıştır. Üç sınıf yedi familyaya ait yirmi yedi bakteri türünden oluşan toplam seksen beş doğal bakteri izolatı tanımlanmış ve bu izolatların hepsi Minimum İnhibitör Konsantrasyon (MİK) açısından ham petrole karşı taranmıştır. Emülsifikasyon indeksi (E₂₄) testi için ham petrole karşı direnç gösteren bakteri izolatları seçilmiştir. E₂₄testlerinde yüksek değerler gösteren izolatlar ileri parçalama testlerinde pH ve petrol katman kalınlığı değerleri kaydedilmek üzere seçilmiştir. Seksen beş izolattan elli tanesi petrol hidrokarbonuna karşı dirençli olarak kaydedilmiştir. Ham petrole karşı pozitif reaksiyon gösteren izolatların yüzdeleri %25 ile %100 arasında değişiklik göstermiştir. S49-Stenotrophomonas maltophila, S50-Aeromonas hydrophila, S59-E. coli1, S38-Aeromonas hydrophila and S43-Enterobacter cloaceae suşları en yüksek Emülsifikasyon İndeksi yüzdeleri gösteren izolatlar olarak kaydedilmiştir. Parçalanma testinin sonuçları, S59-E coli1 izolatının test edilen tüm izolatlardan daha yüksek parçalama kabiliyetine sahip olduğunu göstermiştir. Çalışmanın ilk yılında yapılan ham petrole karşı dirençlilik testlerinde taranan bakterilerin tümünün duyarlı olduğu kaydedilmiştir. Daha sonraki testlerde petrole karşı direnç gösteren bakterilerin tespit edilmesi aynı dönemde Sapanca Gölü’nde meydana gelen beklenmedik petrol sızıntısı ile ilişkilendirilmiştir. Ham petrole dirençli izolatlar, aday bakteriler olarak belirlenmiş ve ileride olası biyoremediasyon çalışmalarında kullanılmak amacıyla stoklanmıştır.

Anahtar Kelimeler

Ham petrol,doğal bakteri izolatları,minimum inhibisyon konsantrasyonu,Sapanca Gölü

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Pelin S Çiftçi Türetken

Gülşen Altuğ

Mine Çardak

Kemal Güneş

Publication Date

August 31, 2018

Submission Date

October 20, 2017

Acceptance Date

February 26, 2018

Published in Issue

Year 2018 Volume: 21 Number: 4

DOI

https://doi.org/10.18016/ksudobil.344593

IZ

https://izlik.org/JA47SH42JS

Cite

RIS / Bibtex

APA

Çiftçi Türetken, P. S., Altuğ, G., Çardak, M., & Güneş, K. (2018). Oil Hydrocarbon Degradation Capability of Bacterial Strains Isolated from the Sapanca Lake, Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 21(4), 535-544. https://doi.org/10.18016/ksudobil.344593

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