Perkloretilen’in in vitro Sitotoksik Etkisinin Brine Shrimp Letalite Testi ile Araştırılması

Ümit Ünsal; Tülay Aşkın Çelik

doi:10.18016/ksudobil.407505

EN TR

Perkloretilen’in in vitro Sitotoksik Etkisinin Brine Shrimp Letalite Testi ile Araştırılması

Öz

Perkloroetilen (PERC), kuru temizleme endüstrisinde çözücü, yağ giderici ve temizleyici olarak yaygın bir şekilde kullanılmaktadır. Perkloroetilen, güçlü ve etkili bir temizleyici olup giysilerin buruşmasını ve renginin solmasını engeller. Kuru temizleme dükkanlarının en az üçte ikisi kuru temizleme işlemlerinde perkloroetilen’i kullanmaktadır. Uluslararası Kanser Araştırmaları Ajansı (IARC, 1995) Perkloroetileni insan karsinojeni olarak Grup2A içerisine almıştır ve kurutemizleme endüstrisinde çalışan insanlar için kanserojen olabileceğini belirtmiştir. PERC’in kuru temizlemede çalışan işçilerde cilt lezyonlarının yanı sıra, lenfosarkom, lösemi, kolon, akciğer ve ürogenital kanserlere neden olduğuna dair çalışmalar bulunmaktadır. Bu çalışmada saf Perkloroetilen’in Artemia salina larvaları üzerindeki potansiyel sitotoksik etkisi Brine Shrimp Letalite Testi ile araştırılmıştır. Denemelerde negatif kontrol olarak tuzlu su, çözücü kontrol olarak etanol ve pozitif kontrol olarak da Mitomisin-C (MMC) kullanılmıştır. Çalışmanın sonuçları, LC₅₀değerlerine göre, in vitro ortamda PERC’e 24 saat maruz bırakılan A.salina larvaları üzerindeki sitotoksik etkinin sadece 1000 ppm’lik PERC uygulanması sonucunda ortaya çıktığını, diğer konsantrasyon aralıklarında (10 ve 100 ppm) ise sitotoksik aktivitesinin olmadığını göstermiştir.

Anahtar Kelimeler

Artemia salina,Brine Shrimp Letalite Testi,Perkloroetilen,Sitotoksik etki

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Ümit Ünsal ^*

Tülay Aşkın Çelik

Publication Date

October 31, 2018

Submission Date

March 19, 2018

Acceptance Date

April 16, 2018

Published in Issue

Year 2018 Volume: 21 Number: 5

DOI

https://doi.org/10.18016/ksudobil.407505

IZ

https://izlik.org/JA74KP34CP

Cite

RIS / Bibtex

APA

Ünsal, Ü., & Çelik, T. A. (2018). Perkloretilen’in in vitro Sitotoksik Etkisinin Brine Shrimp Letalite Testi ile Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 21(5), 644-649. https://doi.org/10.18016/ksudobil.407505

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Perkloretilen’in in vitro Sitotoksik Etkisinin Brine Shrimp Letalite Testi ile Araştırılması

Investigation of in vitro Cytotoxic Effects of Perchloroetylene by Using Brine Shrimp Lethality Assay

Abstract

Keywords

Perkloretilen’in in vitro Sitotoksik Etkisinin Brine Shrimp Letalite Testi ile Araştırılması

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

The genotoxic effects of perchloroethylene in human peripheral blood lymphocytes and the possible ameliorative role of α-tocopherol

Perchloroethylene: Genotoxicity and cytotoxicity in human peripheral blood lymphocytes in vitro

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