Linkage Disequilibrium (LD) Analysis in Alfalfa (Medicago sativa L.) Populations Spreading in Different Geographies of the World
Abstract
Cultivated alfalfa
(Medicago sativa L.)
was derived from Medicago sativa
species complex as a result of breeding efforts. New efforts have focused on to determine the
DNA polymorphisms based on molecular markers and to link these polymorphisms
with related phenotype recently. Especially, the relationships between
genotypes and phenotypes are evaluated as Linkage Analysis and Association
Mapping Studies. On the basis of information, in this study, Linkage
Disequilibrium (LD) analysis was also performed using 31 SSR markers for the 70
populations. For the LD analysis, 23 of the 31 markers identified the physical
distances on the 8 chromosomes of the alfalfa. Distances of 103 loci on 8
chromosomes were determined based on Medicago
truncatula genome. A significant (p<0,0001) LD value
was not observed in these populations for the evaluated markers.
Keywords
References
- Alm V, Fang C, Busso CS, Devos KM, Vollan K, Grieg Z, Rognli OA 2003. A linkage map of meadow fescue (Festuca pratensis Huds.) and comparative mapping with other Poaceae species. Theor. Appl. Genet. 108:25–40.
- Branca A, Paape TD, Zhou P, Briskine R, Farmer AD, Mudge J, Bharti AK, Woodward JE, May GD, Gentzbittel L, Ben C, Denny R, Sadowsky MJ, Ronfort J, Bataillon T, Young ND, Tif- fin P 2011. Whole-genome nucleotide diversity, recombination and linkage disequilibrium in the model legume Medicago trun-catula. Proc Natl Acad Sci USA 108:E864–E870.
- Brummer EC 1999. Capturing heterosis in forage crop cultivar development. Crop Sci 39: 943–954.
- Caldwell KS, Russell J, Langridge P, Powell W 2006. Extreme population-dependent linkage disequilibrium detected in an inbreeding plant species, Hordeum vulgare. Genetics 172:557–567.
- Dabney AR, Storey JD 2007. A new approach to intensity-dependent normalization of two-channel microarrays. Biostatistics 8: 128-139.
- Doyle JJ, Doyle JL 1990. Isolation of plant DNA from fresh tissue. Focus 12:13–15.
- Flint-Garcia SA, Thornsberry JM, Buckler ES 2003. Structure of linkage disequilibrium in plants. Annu. Rev. Plant Biol. 54:357–374.
- Herrmann D, Barre P, Santoni S, Julier B 2010. Association of a CONSTANS-LIKE gene to flowering and height in autotetraploit alfalfa. Theor Appl Genet., 121: 865-876.
Details
Primary Language
English
Subjects
-
Journal Section
Research Article
Authors
Doğan İlhan
0000-0003-2805-1638
Publication Date
November 30, 2018
Submission Date
February 20, 2018
Acceptance Date
April 19, 2018
Published in Issue
Year 2018 Volume: 21 Number: 6
