Detection of Extracellular Lipases and Genotypic Identification from Yeast Causing Spoilage of Some Dairy Products Produced in Gaziantep

Semih Tokak; İbrahim Halil Kılıç; Hüsniye Tansel Yalçın; Tuğçe Duran

doi:10.18016/ksutarimdoga.vi.555727

TR EN

Detection of Extracellular Lipases and Genotypic Identification from Yeast Causing Spoilage of Some Dairy Products Produced in Gaziantep

Abstract

Yeasts are usually found in high amounts in dairy products, which show their ability to adapt to substrates rich in protein, lipid, sugar and organic acids. A wide distribution of yeast in dairy products is a result of proteolytic and lipolytic activities. Spoilage yeasts and molds can grow in most processed and raw foods, where environmental conditions are not suitable for most bacteria (low pH, low water activity, aw). Nutrients and oxygen in food are the main factors that determine the type of fungal spoilage. In this study, dairy products samples (yoghurt, cream, butter, curd cheese, Antep cheese) were collected from local markets in Gaziantep province. In our study, a total of 20 yeast strains were isolated from dairy products and investigated for lipase activities in solid media containing tributyrin. Twenty yeast isolates identified by amplification and sequencing of the ITS region using ITS1 and ITS4 primers Yeasts were identified as Kluyveromyces marxianus (8 isolates), Candida intermedia (8 isolates), Pichia fermentans (2 isolates), Yarrowia lipolytica (1 isolate), Kluyveromyces lactis (1 isolate).

Keywords

Spoilage yeast,Extracellular lipase,Genotypic identification

Supporting Institution

Gaziantep University

Project Number

(FEF. 15.03)

Thanks

This work was supported by the Scientific Research Unit of Gaziantep University (FEF. 15.03). We would like to sincerely thank the Scientific Research Unit of Gaziantep University for providing funding and necessary logistics for this research work

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Semih Tokak ^*
0000-0003-2239-0014
Türkiye

İbrahim Halil Kılıç
0000-0002-0272-5131
Türkiye

Hüsniye Tansel Yalçın
0000-0003-4870-6267
Türkiye

Tuğçe Duran
0000-0002-7353-4527
Türkiye

Publication Date

October 31, 2019

Submission Date

April 18, 2019

Acceptance Date

May 31, 2019

Published in Issue

Year 2019 Volume: 22

DOI

https://doi.org/10.18016/ksutarimdoga.vi.555727

IZ

https://izlik.org/JA59YR45TN

Cite

RIS / Bibtex

APA

Tokak, S., Kılıç, İ. H., Yalçın, H. T., & Duran, T. (2019). Detection of Extracellular Lipases and Genotypic Identification from Yeast Causing Spoilage of Some Dairy Products Produced in Gaziantep. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 22, 206-211. https://doi.org/10.18016/ksutarimdoga.vi.555727

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Detection of Extracellular Lipases and Genotypic Identification from Yeast Causing Spoilage of Some Dairy Products Produced in Gaziantep

Gaziantep İline Ait Bazı Süt Ürünlerinde Bozulmaya Neden Olan Mayalardan Ekstraselüler Lipaz Enzimi Aranması ve Lipaz Aktivitesine Sahip Suşların Genotipik İdentifikasyonu

Öz

Anahtar Kelimeler

Detection of Extracellular Lipases and Genotypic Identification from Yeast Causing Spoilage of Some Dairy Products Produced in Gaziantep

Abstract

Keywords

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

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