Investigation of the Cytotoxicity of Fig, Lemon, Olive, and Turnip Exosomes on MCF-7 Cells

Oğuzhan Karaosmanoğlu

doi:10.18016/ksutarimdoga.v22i49454.589845

EN TR

Investigation of the Cytotoxicity of Fig, Lemon, Olive, and Turnip Exosomes on MCF-7 Cells

Abstract

With their nano-sized structure, exosomes are involved in a wide variety of cellular processes such as genetic information flow, immune system modulations, intercellular communication, and pathophysiological changes. The use of exosomes are exponentially growing particularly in the areas of identification of biomarkers, development of nanocarriers for effective drug delivery, and vaccine production. In recent years, edible plant derived exosomes gained much interest with their strong antimicrobial activities, modulatory activities on the intestinal stem cells, and the anticancer activities. In this study, the cytotoxic effects of fig, lemon, olive and turnip derived exosomes were investigated through the neutral red uptake assay. It was observed that 100 µg/mL protein containing lemon and turnip derived exosomes inhibited the cell proliferation significantly, on the other hand, fig and olive derived exosomes did not alter the proliferation of MCF-7 cells. Since the results for the cytotoxic activity of turnip exosomes are original in this research, it was found worthy to emphasis the utility of turnip exosomes for the development of new anticancer agents or new drug delivery nanocarriers.

Keywords

İncir, Limon, Zeytin ve Turp Ekzozomlarının MCF-7 Hücrelerinde Sitotoksik Etkisinin Araştırılması

Öz

Nano-boyutlu yapılarıyla ekzozomlar genetik bilgi akışı, immun sistem düzenlenmesi ve patofizyolojik değişiklikler gibi çok çeşitli hücresel süreçlere dahil olurlar. Ekzozomların, özellikle biyoişaret tanımlanması, etkin ilaç dağıtımı için nanotaşıyıcıların geliştirilmesi ve aşı üretilmesi alanlarında kullanımları hızla artmaktadır. Son yıllarda yenilebilir bitkilerden türevlenen ekzozomlar yüksek antimikrobiyal etkileri, bağırsak kök hücreleri üzerinde düzenleyici etkileri ve antikanser etkileriyle ilgi çekmektedir. Bu çalışmada incir, limon, zetin ve turp türevli ekzozomların sitotoksik etkileri nötral kırmızısı alım testiyle araştırılmıştır. Protein miktarı 100 µg/mL olan limon ve turp ekzozomlarının hücre çoğalmasını engellediği gözlenirken incir ve zeytin türevli ekzozomların MCF-7 çoğalmasını etkilemediği görülmüştür. Turp ekzozomlarının sitotoksistik aktivitesi sonuçları, bu araştırmada orijinal olduğu için, yeni antikanser ajanlarının veya yeni ilaç dağıtım nanotaşıyıcılarının geliştirilmesi için turp ekzozomları kullanımına vurgu yapmanın değerli olabileceği düşünülmüştür.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Oğuzhan Karaosmanoğlu ^*
0000-0003-2028-7339
Türkiye

Publication Date

December 31, 2019

Submission Date

July 10, 2019

Acceptance Date

August 23, 2019

Published in Issue

Year 2019 Volume: 22

DOI

https://doi.org/10.18016/ksutarimdoga.v22i49454.589845

IZ

https://izlik.org/JA67TT79FM

Cite

RIS / Bibtex

APA

Karaosmanoğlu, O. (2019). Investigation of the Cytotoxicity of Fig, Lemon, Olive, and Turnip Exosomes on MCF-7 Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 22, 382-387. https://doi.org/10.18016/ksutarimdoga.v22i49454.589845

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AAPS PharmSciTech

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Investigation of the Cytotoxicity of Fig, Lemon, Olive, and Turnip Exosomes on MCF-7 Cells

Investigation of the Cytotoxicity of Fig, Lemon, Olive, and Turnip Exosomes on MCF-7 Cells

Abstract

Keywords

İncir, Limon, Zeytin ve Turp Ekzozomlarının MCF-7 Hücrelerinde Sitotoksik Etkisinin Araştırılması

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

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