Siklofosfazen Türevlerinin Maya Kültür Ortamlarında Malondialdehit, Glutatyon ve Total Protein Miktarları Üzerine Etkileri

Ayşe Dilek Özşahin; Derya Beşer; Ali İhsan Öztürk; Fatih Aslan; Ökkeş Yılmaz

doi:10.18016/ksutarimdoga.vi.600569

EN TR

Siklofosfazen Türevlerinin Maya Kültür Ortamlarında Malondialdehit, Glutatyon ve Total Protein Miktarları Üzerine Etkileri

Öz

Bu çalışmada; yeni sentezlenmiş beş schiff bazının S. cerevisiae kültür ortamlarında toplam protein, glutatyon (GSH) ve malondialdehit (MDA) analizleri yapıldı. S. cerevisiae’nın gelişimi ve çoğalması için YEPD besiyeri ortamı hazırlandı. Uygulama grupları için; schiff bazların her birinden 10 mg/mL ’lık ve 20 mg/mL ’lık konsantrasyonları kültür ortamına ilave edildi. İnkübasyon sonunda elde edilen besiyeri ile toplam protein, glutatyon (GSH), okside-glutatyon (GSSG) ve MDA analizleri yapıldı. Kontrol grubu ile schiff bazı gruplarının sonuçları karşılaştırıldığında; yeni sentezlenmiş schiff bazlarının S. cerevisiae’da; toplam protein, GSH ve MDA miktarlarını arttırdığı belirlendi. Sonuç olarak; yeni sentezlenmiş schiff bazlarının özellikle antioksidan savunma etkisi ve sitotoksik özelliği ile gelecekte hastalık tedavilerine yönelik yapılacak olan çalışmalara destek olacağı ve literatüre katkı sağlayacağı düşünülmektedir.

Anahtar Kelimeler

Glutatyon,MDA,Saccharomyces cerevisiae,Schiff bazı,Toplam Protein

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Details

Primary Language

Turkish

Subjects

Structural Biology

Journal Section

Research Article

Authors

Ayşe Dilek Özşahin ^*
0000-0002-1832-7082
Türkiye

Derya Beşer
0000-0002-8592-6261
Türkiye

Ali İhsan Öztürk
0000-0002-3912-0670
Türkiye

Fatih Aslan
0000-0002-5948-6979
Türkiye

Ökkeş Yılmaz
0000-0002-8276-4498
Türkiye

Publication Date

April 30, 2020

Submission Date

August 2, 2019

Acceptance Date

November 28, 2019

Published in Issue

Year 2020 Volume: 23 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.600569

IZ

https://izlik.org/JA74ZC33EY

Cite

RIS / Bibtex

APA

Özşahin, A. D., Beşer, D., Öztürk, A. İ., Aslan, F., & Yılmaz, Ö. (2020). Siklofosfazen Türevlerinin Maya Kültür Ortamlarında Malondialdehit, Glutatyon ve Total Protein Miktarları Üzerine Etkileri. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(2), 281-288. https://doi.org/10.18016/ksutarimdoga.vi.600569

Cited By

Saccharomyces cerevisiae L. Hücre Kültürü Oksidatif Stres Modelinde Bazı Fosfazen Türevlerinin Biyokimyasal Aktiviteleri

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1051663

Siklofosfazen Türevlerinin Maya Kültür Ortamlarında Malondialdehit, Glutatyon ve Total Protein Miktarları Üzerine Etkileri

Effects of Cyclophosphazene Derivatives on Malondialdehyde, Glutathione and Total Protein Content in Yeast Culture Media

Abstract

Keywords

Siklofosfazen Türevlerinin Maya Kültür Ortamlarında Malondialdehit, Glutatyon ve Total Protein Miktarları Üzerine Etkileri

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Saccharomyces cerevisiae L. Hücre Kültürü Oksidatif Stres Modelinde Bazı Fosfazen Türevlerinin Biyokimyasal Aktiviteleri