Overexpression of Cytochrome P450 CYP6CM1 Gene in Bemisia tabaci (Gennadius) in the Cotton Fields of the Çukurova Region, Turkey

Selçuk Ulusoy

doi:10.18016/ksutarimdoga.vi.605716

TR EN

Çukurova Bölgesi Pamuk Alanlarında Bemisia tabaci (Gennadius)’de Aşırı Düzeyde Sitokrom P450 CYP6CM1 Gen Ekspresyonu

Öz

Çukurova bölgesi polikültür tarımın yapıldığı bir bölgedir. Bu bölgede pamuk ekim alanlarında B. tabaci mücadelesinde neonikotinoid grubu insektisitler yoğun bir şekilde kullanılmakta olup, direnç problemi ile karşılaşılmaktadır. Çalışmada 2018 yılı, Adana ili pamuk alanlarından toplanmış beş farklı B. tabaci popülasyonunda imidacloprid dayanıklılığı göstergesi olan sitokrom P450 CYP6CM1 geni relatif expresyon düzeyleri incelenmiştir. Ayrıca metabolik dayanıklılıkda etkili GST geni relatif expresyon profili, sitokrom P450, karboksil esteraz(AcHE) ve Glutathion S transferaz (GST) enzim etkinlikleri belirlenmiştir. En yüksek relatif aktivite Meletmez populasyonunda CYP6CM1 geni 18.2 kat ve Gst geni 9 kat daha fazla gen regülasyonu belirlenmiştir. Ayrıca en yüksek ve en düşük sitokrom P450 enzim aktivitesi meletmez ve solaklı popülasyonlarında gözlemlenmiştir. Yapılan analizlerde Çukurova bölgesi pamuk alanlarında B. tabaci popülasyonlarında yaygın imidacloprid ve neonikotinoid dayanıklılığı durumundan bahsedilebilir. Sonuç olarak, aşırı gen expresyonu ve yüksek düzeydeki enzim etkinlikleri, bölgede güçlendirilmiş monooksigenaz etkinliğine dayanan direncin varlığını ortaya koymaktadır.

Anahtar Kelimeler

Bemisia tabaci,Pamuk,Sitokrom P450,Aşırı ekspresyon

Overexpression of Cytochrome P450 CYP6CM1 Gene in Bemisia tabaci (Gennadius) in the Cotton Fields of the Çukurova Region, Turkey

Abstract

In Cukurova region, neonicotinoid group insecticides are widely used in cotton cultivation areas for controlling B. tabaci; as a result of this use, there is a problem of resistance in these areas. In this study, cytochrome P450 CYP6CM1 relative expression levels, which are the indicators of imidacloprid resistance, were analyzed. Five different B. tabaci populations were collected from Adana city cotton cultivation areas in 2018 and the indicators were observed. On the other hand, GST gene relative expression profile, which is an efficient factor in metabolic resistance, cytochrome P450, carboxylesterase (AcHE) and Glutathion S transferase (GST) enzyme activities were determined. The highest cyp6cm1 and GST genes relative activities were determined in Meletmez population; the activity level was 18, 2 and 9 times higher than susceptible population. On the other hand, the highest and lowest cytochrome P450 enzyme activity was observed in Meletmez and Solaklı populations, respectively. According to the analyses, it can be concluded that there is extensive imidacloprid and neonicotinoid resistance of B. tabaci populations in Çukurova Region cotton cultivation areas. As a result, excessive gene expression and high-level enzyme activities indicate that there is a resistance in the studied area based on enforced monooxygenases activity.

Keywords

Bemisia tabaci,Cotton,Cytochrome P450,Overexpression

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Selçuk Ulusoy ^*
0000-0002-2278-8471
Türkiye

Publication Date

June 30, 2020

Submission Date

August 16, 2019

Acceptance Date

January 21, 2020

Published in Issue

Year 2020 Volume: 23 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.605716

IZ

https://izlik.org/JA39CM55JG

Cite

RIS / Bibtex

APA

Ulusoy, S. (2020). Overexpression of Cytochrome P450 CYP6CM1 Gene in Bemisia tabaci (Gennadius) in the Cotton Fields of the Çukurova Region, Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(3), 634-640. https://doi.org/10.18016/ksutarimdoga.vi.605716

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