Diyarbakır İlinde Yetişen Badem Ağaçlarından Endofit ve Epifit Bakteri Türlerinin İzolasyonu ve Bitki Gelişimini Teşvik Eden Mekanizmalarının Karakterizasyonu

Zekiye Ceren Aktan; Soner Soylu

doi:10.18016/ksutarimdoga.vi.659802

EN TR

Prevalence and Characterization of Plant Growth Promoting Mechanisms of Endophytic and Epiphytic Bacterial Species Isolated from Almond Trees Growing in Diyarbakır Province of Turkey

Abstract

The aims of this study were to identify endophytic and epiphytic plant growth promoting bacteria (PGPB) from healthy almond trees and to characterize plant growth promoting mechanisms. By using MALDI-TOF analyses, 19 Gram-negative isolates, belonging to Serratia, Pseudomonas, Ochrobactrum and Enterobacter genus and 26 Gram-positive isolates, belonging to Bacillus and Arthrobacter genus were identified. Among the tested bacterial isolates, 46 isolates were positive for siderophore production, 45 isolates for ammonium production, 35 isolates solubilized phosphorus varying ratios, and all isolates produced IAA between 5.48-127.81 µg/ml concentrations. Isolates of Pseudomonas spp and Enterobacter cloacae were found highly effective for siderophore production, S. marcescens for solubilisation of phosphorus, E. cloacae for IAA production, Bacillus spp for ammonia production. Bacterial isolates tested showed significant differences in siderophore, ammonia and IAA production and phosphorus solubilisation efficiency. It was found that the efficacies of endophytic bacterial isolates were lower compared to the same species of epiphytic bacterial isolates. Overall results suggest that use of the most efficient PGPB isolates have an excellent potential to be used as biofertilizer for cultivation of economically important crops.

Keywords

Almond,Biological control,Plant Growth Promoting Bacteria (PGPB),Bio-fertiliser

Diyarbakır İlinde Yetişen Badem Ağaçlarından Endofit ve Epifit Bakteri Türlerinin İzolasyonu ve Bitki Gelişimini Teşvik Eden Mekanizmalarının Karakterizasyonu

Öz

Bu çalışmanın amacı, sağlıklı badem ağaçlarından izole edilen bitki gelişimini teşvik eden endofit ve epifit bakteri (PGPB) türlerin tanılanması ve bitki gelişimini teşvik eden mekanizmalarının belirlemektir. MALDI-TOF tanılama analizleri sonucu, 19 izolat Gram-negatif Serratia, Pseudomonas, Ochrobactrum ve Enterobacter, 26 izolat ise Gram-pozitif Bacillus ve Arthrobacter cinsine dahil türler olarak teşhis edilmiştir. Test edilen izolatlardan, 46 izolat siderofor, 45 izolat amonyak üretiminde pozitif, 35 izolat ise fosforu değişen oranlarda çözebilme yeteneğinde olurken, bakterilerin tamamı 5.48-127.81 µg/ml konsantrasyon aralığında IAA üretmişlerdir. Pseudomonas spp and Enterobacter cloacae izolatları siderofor, Serratia marcescens izolatları fosforu çözme, Enterobacter cloacae izolatları IAA üretme, Bacillus spp ise amonyak üretme etkinliğinin yüksek düzeylerde olduğu görülmüştür. Aynı türe bağlı endofit bakteri izolatları epifit bakteri izolatlarına kıyasla bu mekanizmaları daha düşük düzeyde teşvik ettiği belirlenmiştir. Elde edilen sonuçlar yüksek düzeyde siderofor, amonyak, IAA üretimi ile fosfor çözme gibi bitki gelişimini teşvik edici özellikleri sahip PGPB izolatlarının ekonomik öneme sahip kültür bitkilerin yetiştiriciliğinde biyolojik gübre olarak kullanılmada potansiyeline sahip olduğunu göstermiştir.

Anahtar Kelimeler

Badem,Biyolojik Mücadele,Bitki Gelişimini Teşvik Eden Bakteri (PGPB),Biyogübre

Supporting Institution

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı

Project Number

MKU BAP-16444

Thanks

Bu çalışma Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı tarafından finansal olarak desteklenmiştir (Proje Numarası: MKU BAP-16444).

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Details

Primary Language

Turkish

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Zekiye Ceren Aktan
0000-0003-1031-8314
Türkiye

Soner Soylu ^*
0000-0003-1002-8958
Türkiye

Publication Date

June 30, 2020

Submission Date

December 16, 2019

Acceptance Date

February 24, 2020

Published in Issue

Year 2020 Volume: 23 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.659802

IZ

https://izlik.org/JA38YJ35ZD

Cite

RIS / Bibtex

APA

Aktan, Z. C., & Soylu, S. (2020). Diyarbakır İlinde Yetişen Badem Ağaçlarından Endofit ve Epifit Bakteri Türlerinin İzolasyonu ve Bitki Gelişimini Teşvik Eden Mekanizmalarının Karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(3), 641-654. https://doi.org/10.18016/ksutarimdoga.vi.659802