Evaluation of F5 Individuals Obtained from B28×Kunduru-1149 Reciprocal Cross Population by Functional Markers

Gülan Aydemir; Ziya Dumlupınar; İlker Yüce; Tuğba Narlı; Selçuk Sunulu; Hüseyin Güngör

doi:10.18016/ksutarimdoga.vi.687935

TR EN

B28×Kunduru-1149 Resiproklu Melez Popülasyonundan Elde Edilen F5 Bireylerinin Fonksiyonel Markörlerle Değerlendirilmesi

Öz

Bu çalışmada, B28 ve Kunduru-1149 makarnalık buğday genotipleri 2012-2013 ürün sezonunda resiproklu olarak melezlenmiş ve elde edilen 13 B28/Kunduru-1149-Kunduru-1149/B28 F5 kademesindeki hat materyal olarak kullanılmıştır. Melez kombinasyonları ve ebeveynler gluten dayanıklılığı (Bx7OE), sarı pas (Sun104, Xgwm18, Xwgp115 ve Xgwm47), kara pas (Sun209 ve Sun479), yüksek protein oranı (UHW89), külleme (Xgwm66) ve kahverengi pas (Xgwm130) allelleri bakımından 10 DNA markörü ile taranmıştır. Çalışmada, ortalama polimorfizmi bilgi içeriği (PIC) 0.98 olarak hesaplanmış ve en düşük PIC değeri 0.96 ile Xwgp115 marköründen elde edilirken, diğer markörler 0.99 PIC değerine sahip olmuştur. B28/Kunduru-1149_F5_4 (Sun479) ve B28/Kunduru-1149_F5_1 (Sun209) genotiplerinde kara pasa dayanıklılık alleli Sr49 tespit edilmiştir. Sarı pas dayanıklılık allellerinden Yr15 (Xgwm18) B28/Kunduru-1149_F5_2 ve B28/Kunduru-1149_F5_3 genotiplerinde bulunurken, Yr51 (Sun104) geni B28/Kunduru-1149_F5_3, B28/Kunduru-1149_F5_6, B28/Kunduru-1149_F5_7, Kunduru-1149/B28_F5_2 ve Kunduru-1149/B28_F5_6 genotiplerinde tanımlanmıştır. Ebeveynler ile genotiplerin akrabalıklarını belirlemek için bir dendrogram oluşturulmuştur. En yüksek genetik benzerlik oranı B28 / Kunduru-1149_F5_6 ve Kunduru-1149 / B28_F5_2 genotipleri arasında 0.714 olarak tespit edilirken, birbirlerine en uzak genotipler 0.10 benzerlik oranı ile Kunduru-1149 ve B28/Kunduru_F5_7 olmuştur.

Anahtar Kelimeler

Makarnalık buğday,Fonksiyonel markörler,Sarı pas,Kara pas,Gluten mukavemeti

Evaluation of F5 Individuals Obtained from B28×Kunduru-1149 Reciprocal Cross Population by Functional Markers

Abstract

In the study, B28 and Kunduru-1149 durum wheat genotypes were crossed as reciprocal in 2012-2013 cropping season. 13 (B28/Kunduru-1149 and Kunduru-1149/B28) reciprocal crosses were obtained and were used as materials at F5 stage. The cross combinations and the parents were screened with 10 DNA markers to determine alleles of gluten strength (Bx7OE), Yellow rust (Sun104, Xgwm18, Xwgp115 and Xgwm47), stem rust (Sun209 and Sun479), high protein ratio (UHW89), powdery mildew (Xgwm66) and leaf rust (Xgwm130). In the study, the average polymorphism information content (PIC) was calculated as 0.98 and the lowest PIC value was obtained from Xwgp115 marker with 0.96, while the rest of the markers had 0.99 PIC values. Stem rust resistance allele Sr49 was detected in B28/Kunduru-1149_F5_4 (Sun479) and B28/Kunduru-1149_F5_1 (Sun209) genotypes. One of the yellow rust resistance alleles Yr15 (Xgwm18) was detected in B28/Kunduru-1149_F5_2 and B28/Kunduru-1149_F5_3 genotypes, while Yr51 (Sun104) was identified in B28/Kunduru-1149_F5_3, B28/Kunduru-1149_F5_6, B28/Kunduru-1149_F5_7, Kunduru-1149/B28_F5_2 and Kunduru-1149/B28_F5_6 genotypes. A dendrogram was created to determine kinship of the individuals with the parents. The highest genetic similarity was observed between B28 / Kunduru-1149_F5_6 and Kunduru-1149 / B28_F5_2 genotypes with 0.714, while the most diverse ones were Kunduru-1149 and B28/Kunduru_F5_7 with 0.10.

Keywords

Durum wheat,Functional markers,Yellow rust,Stem rust,Gluten strength

Supporting Institution

The Scientific Research Projects Council of Kahramanmaraş Sütçü İmam University

Project Number

2017/7-175 YLS

Thanks

This study is produced from the master thesis of Gülan AYDEMİR. The other authors were responsible for making crosses and bringing generations from F1 to F5. The abstract is also published in International Biological, Agricultural & Life Science Congress November 7-8, 2019 Lviv, Ukraine. Authors also would like to thank The Scientific Research Projects Council of Kahramanmaraş Sütçü İmam University (Project Number 2017/7-175 YLS).

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Gülan Aydemir
0000-0001-6054-8893
Türkiye

Ziya Dumlupınar ^*
0000-0001-6054-8893
Türkiye

İlker Yüce
0000-0002-9761-3561
Türkiye

Tuğba Narlı
0000-0002-0744-6086
Türkiye

Selçuk Sunulu
0000-0002-1806-2547
Türkiye

Hüseyin Güngör
0000-0001-6708-6337
Türkiye

Publication Date

August 31, 2020

Submission Date

February 11, 2020

Acceptance Date

April 24, 2020

Published in Issue

Year 2020 Volume: 23 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.687935

IZ

https://izlik.org/JA75KC44SM

Cite

RIS / Bibtex

APA

Aydemir, G., Dumlupınar, Z., Yüce, İ., Narlı, T., Sunulu, S., & Güngör, H. (2020). Evaluation of F5 Individuals Obtained from B28×Kunduru-1149 Reciprocal Cross Population by Functional Markers. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(4), 1005-1011. https://doi.org/10.18016/ksutarimdoga.vi.687935

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