The Relation between Biochemical Parameters, Milk Amyloid A, Somatic Cell Count, and Some Pathogens in Buffalo Milks

Yağmur Nil Doğan; Mürüvvet Düz; İlkay Doğan; Zeki Gürler

doi:10.18016/ksutarimdoga.vi.689535

TR EN

Manda Sütlerindeki Somatik Hücre Sayısının Bazı Patojenler, Biyokimyasal Parametreler ve Süt Amiloid A ile İlişkisi

Öz

Mandaların olumsuz çevre koşullarına, hastalıklara karşı dirençli yapısı sütünü de değerli kılmaktadır. Manda sütlerine mastitis, çevresel bulaşma, stres gibi istenmeyen bazı durumlara bağlı olarak mikrobiyel bulaşma olabilir. Bu mikroorganizmaların sütten uzaklaştırılamaması sonucunda yetersiz üretim, fermantasyonun gerçekleşmemesi, ürünlerin raf ömrünün kısalması gibi üretim sorunlarına neden olmaktadır. Bu nedenle, manda sütlerindeki somatik hücre sayısının bazı patojenlerin varlığı ve süt amiloid A ile ilişkisinin belirlenmesi amaçlanmıştır. Buna ilaveten manda sütlerinde meydana gelen oksidatif stres değerlendirilmiştir. Bu amaç doğrultusunda, toplanan manda sütlerinde Enterobacteriaceae, koliform mikroorganizmalar, Escherichia coli ve Salmonella spp. analizleri yapılmıştır. Biyokimyasal parametrelerden malondialdehit (MDA), glutatyon (GSH), nitrik oksit (NOx), katalaz (KAT), süperoksit dismutaz (SOD), total antioksidan seviye (TAS) ölçülmüştür. Serolojik olarak ise süt amiloid A (SAA) seviyesi belirlenmiştir. Bu analiz sonuçlarına göre Enterobacteriaceae, koliform mikroorganizma, E. coli gruplarına göre SHS ortalamaları istatistiksel olarak anlamlı farklılık göstermemiştir. MDA, NOx, SOD, TAS değerleri SHS gruplarına göre istatistiksel farklılık göstermezken; GSH ve KAT değerlerinin SHS gruplarına göre istatistiksel olarak farklı olduğu tespit edilmiştir. SAA değeri SHS gruplarına göre istatistiksel olarak anlamlı farklılık göstermiştir. Buna ilaveten SAA ile MDA ve SHS değeri arasında istatistiksel olarak anlamlı, düşük düzeyde, pozitif yönde bir korelasyon tespit edilmiştir. Manda sütlerinde süt amiloid A’nın tespiti ile, hastalıklı hayvan sütlerinin sağlıklı sütlerle karışmasına engel olunarak daha güvenilir manda süt ve süt ürünleri tüketime sunulma olanağı kazandırır.

Anahtar Kelimeler

The Relation between Biochemical Parameters, Milk Amyloid A, Somatic Cell Count, and Some Pathogens in Buffalo Milks

Abstract

Being resistant to hard environmental conditions and diseases makes Buffalo to have its valuable milk. Microbial contamination may occur due to undesirable conditions such as mastitis, environmental contamination, and stress. If microorganisms are not removed from the milk, it causes many production disadvantages including inadequacy of production, failure of fermentation and shortening of the shelf life. This study was conducted to determine the relationship between somatic cells count (SCC), the presence of some pathogens, and milk amyloid A (MAA) in the buffalo milk. In addition, oxidative stress in buffalo milk was evaluated. For this purpose, 70 samples were collected and Enterobacteriaceae, coliform microorganisms, Escherichia coli, Salmonella spp. analyses were performed. Biochemical parameters [Malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO), catalase, superoxide dismutase (SOD), and total antioxidant capacity (TAC)] and serological parameter (MAA) were measured. The SCC was not statistically different according to Enterobacteriaceae, coliform microorganisms, E. coli groups. While MDA, NO, SOD, and TAC values were not statistically different according to the SCC groups, GSH and catalase values were statistically different. MAA values were statistically significant compared to the SCC groups. Moreover, there was a positive correlation between MAA and MDA/SCC. Detection of MAA may prevent the mixing of healthy and mastitic milk. Therefore, more reliable buffalo milk products will be presented to consumption.

Keywords

Supporting Institution

Afyon Kocatepe Üniversitesi

Project Number

17.VF.01

Thanks

This study was financially supported by Afyon Kocatepe University, Scientific Projects Research Coordination Centre as 17.VF.01 project number.

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Details

Primary Language

English

Subjects

Structural Biology , Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Yağmur Nil Doğan ^*
0000-0002-1309-0936
Türkiye

Mürüvvet Düz
0000-0001-8032-4280
Türkiye

İlkay Doğan
0000-0001-7552-6478
Türkiye

Zeki Gürler
0000-0002-9037-2945
Türkiye

Publication Date

October 31, 2020

Submission Date

February 14, 2020

Acceptance Date

April 9, 2020

Published in Issue

Year 2020 Volume: 23 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.689535

IZ

https://izlik.org/JA29CD26UT

Cite

RIS / Bibtex

APA

Doğan, Y. N., Düz, M., Doğan, İ., & Gürler, Z. (2020). The Relation between Biochemical Parameters, Milk Amyloid A, Somatic Cell Count, and Some Pathogens in Buffalo Milks. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(5), 1379-1385. https://doi.org/10.18016/ksutarimdoga.vi.689535

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