In vitro Cytotoxic Effect of Ethanol and Dimethyl Sulfoxide on Various Human Cell Lines

Elif Ayazoglu Demir; Selim Demir; Yüksel Aliyazıcıoğlu

doi:10.18016/ksutarimdoga.vi.702702

TR EN

Etanol ve Dimetil Sülfoksidin Çeşitli İnsan Hücre Hatları Üzerindeki in vitro Sitotoksik Etkisi

Öz

Literatürde etanol ve dimetil sülfoksit (DMSO)’in bazı hücre hatları üzerindeki sitotoksik etkileri gösterilmiş olmasına rağmen, bu çözücülerin melanoma (VMM917), akciğer kanseri (A549), kolon kanseri (WiDr), normal kolon (CCD 841 CoN) ve fibroblast (BJ) hücreleri üzerindeki sitotoksik etkilerini belirleyen bir çalışma bulunmamaktadır. Bu çalışmanın amacı, etanol ve DMSO'nun yukarıda belirtilen hücrelere ek olarak insan meme (MCF-7), karaciğer (HepG2) ve serviks (HeLa) kanser hücreleri üzerindeki sitotoksik etkilerini belirlemektir. Bu amaçla, hücreler farklı konsantrasyonlarda (%0.1, %0.2, %0.4, %0.6, %0.8, %1, %2 ve % 4, hacim/hacim) etanol ve DMSO ile muamele edilmiş ve daha sonra MTT prosedürü uygulanmıştır. Elde edilen sonuçlar, etanol ve DMSO’nun incelenen tüm hücrelerde doza bağlı sitotoksisite sergilediğini göstermiştir. DMSO ve etanole en duyarlı hücreler sırasıyla WiDr ve VMM917 iken, en dirençli hücreler sırasıyla BJ ve A549 olarak belirlenmiştir. Sonuçlar, her bir hücre hattında etanol ve DMSO'nun sitotoksik etki sergilediği konsantrasyon aralığının farklı olduğunu ortaya koymuştur. Yanlış pozitif ve negatif sonuçlara neden olmamak için, hücre kültürü çalışmalarında öncelikle kullanılacak çözücülerin sitotoksik etkilerinin oluşmadığı konsantrasyon aralığı belirlenmelidir.

Anahtar Kelimeler

Hücre kültürü,Sitotoksisite,Etanol,Dimetil sülfoksit,MTT yöntemi

In vitro Cytotoxic Effect of Ethanol and Dimethyl Sulfoxide on Various Human Cell Lines

Abstract

Although the cytotoxic effects of ethanol and dimethyl sulfoxide (DMSO) on some cell lines have been shown in the literature, there is no study about the cytotoxic effects of these solvents on common used human cell lines, such as melanoma (VMM917), lung cancer (A549), colon cancer (WiDr), normal colon (CCD 841 CoN) and fibroblast (BJ) cells. The aim of this study was to determine the cytotoxic effects of ethanol and DMSO on human breast (MCF-7), liver (HepG2) and cervix (HeLa) cancer cells in addition to above mentioned cells. For this purpose, the cells were treated with different concentrations (0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1%, 2%, and 4%, v/v) of ethanol and DMSO and then subjected to MTT assay. According to the results, ethanol and DMSO exhibited dose-dependent cytotoxicity in all cells studied. The most DMSO and ethanol sensitive cells were WiDr and VMM917, while the most resistant cells were determined as BJ and A549, respectively. The results revealed that the concentration range in which ethanol and DMSO exhibited cytotoxic effect in each cell line is different. In order not to cause false positive and negative results, the concentration range in which the solvents used in cell culture studies do not have cytotoxic effects should be determined.

Keywords

Cell culture,Cytotoxicity,Ethanol,Dimethyl sulfoxide,MTT assay

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Elif Ayazoglu Demir
0000-0001-9027-7633
Türkiye

Selim Demir ^*
0000-0002-1863-6280
Türkiye

Yüksel Aliyazıcıoğlu
0000-0001-9474-4307
Türkiye

Publication Date

October 31, 2020

Submission Date

March 12, 2020

Acceptance Date

April 24, 2020

Published in Issue

Year 2020 Volume: 23 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.702702

IZ

https://izlik.org/JA36ED37RX

Cite

RIS / Bibtex

APA

Ayazoglu Demir, E., Demir, S., & Aliyazıcıoğlu, Y. (2020). In vitro Cytotoxic Effect of Ethanol and Dimethyl Sulfoxide on Various Human Cell Lines. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(5), 1119-1124. https://doi.org/10.18016/ksutarimdoga.vi.702702