The Determination of In vitro Antioxidant and Cytotoxic Activities of Resin Obtained from Cilician Fir (Abies cilicica (Antoine & Kotschy) Carrière)

Esra Uçar Sözmen; Serap Şahin Bölükbaşı; Mustafa Ulu; Aşkın Akpolat

doi:10.18016/ksutarimdoga.vi.720675

TR EN

Toros Göknarı (Abies cilicica (Antoine & Kotschy) Carrière) ’ndan Elde Edilen Reçinenin In vitro Antioksidan ve Sitotoksik Aktivitelerinin Belirlenmesi

Öz

Bu çalışmada, Toros Göknarı bitkisinden elde edilen reçinenin antioksidan ve sitotoksik aktivite değerleri değerlendirilmiştir. Verilere göre, reçinenin in vitro antioksidan aktivitesi, radikal süpürücü aktivite yöntemine bağlı olarak değişmiştir. Bu reçine, 2,2′-Azino-bis- (3-etilbenzotiazolin-6-sülfonik asit) (ABTS) radikal süpürme testine göre antioksidan aktiviteye sahiptir. Reçinenin in vitro sitotoksik aktivitesi, bir dizi insan kanser hücresine (MDA-MB-231, Hep G2, PC-3, U-87, MCF-7, HT-29) karşı MTT (3- (4,5-dimetiltiyazol-2-il) -2,5-difeniltetrazolium bromid) analizi ile 48 saat boyunca araştırıldı. Sağlıklı hücreler olarak normal insan akciğer fibroblast hücreleri (WI-38) kullanıldı. Sonuçlar, reçinenin in vitro sitotoksik aktivitesinin hücre hattı tipine ve numunenin konsantrasyonuna bağlı olduğunu göstermiştir. IC50 değerlerine göre, reçine diğer kanser hücrelerine kıyasla endometriyal adenokarsinom kanser hücreleri üzerinde en fazla sitotoksik aktiviteye sahiptir (IC50 = 8.94 ± 0.03 µg mL-1). Sonuçlar ayrıca seçicilik indeks (SI) değeri> 2 olan Ishikawa endometriyal adenokarsinom hücrelerinin reçineye karşı en yüksek hassasiyete sahip olduğunu göstermiştir. Bu çalışma, reçinenin farklı kanser hücrelerinin büyümesini engellediğine dair ilk kanıtları sunmaktadır.

Anahtar Kelimeler

Toros Göknarı,Abies,Antioksidan,Antikanser,Sitotoksisite

Thanks

The authors would like to acknowledge the contribution of the late Hanife Öğütlü for plant resin support in the realization of these studies. We hope that she rests in peace. Cytotoxic activity studies were conducted at Sivas Cumhuriyet University Advanced Technology Application and Research Center (CUTAM).

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Esra Uçar Sözmen ^*
0000-0001-6327-4779
Andorra

Serap Şahin Bölükbaşı
0000-0003-1057-2558
Türkiye

Mustafa Ulu
0000-0003-4503-3036
Türkiye

Aşkın Akpolat
0000-0001-8394-2746
Türkiye

Publication Date

December 31, 2020

Submission Date

April 16, 2020

Acceptance Date

May 31, 2020

Published in Issue

Year 1970 Volume: 23 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.720675

IZ

https://izlik.org/JA69LN98XN

Cite

RIS / Bibtex

APA

Uçar Sözmen, E., Şahin Bölükbaşı, S., Ulu, M., & Akpolat, A. (2020). The Determination of In vitro Antioxidant and Cytotoxic Activities of Resin Obtained from Cilician Fir (Abies cilicica (Antoine & Kotschy) Carrière). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(6), 1503-1509. https://doi.org/10.18016/ksutarimdoga.vi.720675

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The Determination of In vitro Antioxidant and Cytotoxic Activities of Resin Obtained from Cilician Fir (Abies cilicica (Antoine & Kotschy) Carrière)

Toros Göknarı (Abies cilicica (Antoine & Kotschy) Carrière) ’ndan Elde Edilen Reçinenin In vitro Antioksidan ve Sitotoksik Aktivitelerinin Belirlenmesi

Öz

Anahtar Kelimeler