Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis sativus L.) Seedling via Morphological and Molecular Methods

Hüseyin Bulut; Halil İbrahim Öztürk; Atilla Dursun

doi:10.18016/ksutarimdoga.vi.754689

TR EN

Tebuconazole Uygulamalarının Hıyar (Cucumis sativus L.) Fidelerindeki Etkisinin Morfolojik ve Moleküler Yöntemler ile Belirlenmesi

Abstract

Çalışmada, Tebuconazole uygulamalarının hıyar (Cucumis sativus L.) fidelerindeki etkisinin morfolojik ve moleküler yöntemler ile belirlenmesi amaçlanmıştır. Yapraktan sprey şeklinde uygulanan Tebuconazole’ün 25, 50, 75, 100, 125 ve 150 ppm konsantrasyonları denenmiştir. Fide kalitesine olumlu etki eden en uygun dozun belirlenmesi ve muhtemel genotoksik etkilerin engellenmesi için tüm dozlardaki retrotranspozon hareketliliği tespit edilmiştir. Ayrıca oluşan polimorfizm oranları ve GTS değerlerindeki değişim belirlenmiştir. Uygulanan dozlarda sırasıyla %0, %5.55, %16.66, %38.88, %55.55 ve %61.11 oranında polimorfizm elde edilmiştir. GTS değerinin ise doza bağlı olarak %100 seviyesinden %38.89 seviyesine gerilediği görülmüştür. Yapılan istatiksel değerlendirmelere göre, farklı doz uygulamalarının fide özelliklerinde önemli derecede değişiklik meydana getirdiği tespit edilmiştir. Elde edilen verilere göre, kontrol uygulaması ile karşılaştırıldığında, fide boyu ve gövde boyunda sırası ile %57,5 ve %55,9’luk bir azalma sağlanmıştır. Gövde çapında ise uygulama sonrası %9.95’lik bir artış olmuştur. 50, 75 ve 150 ppm’lik dozların gövde kuru madde oranında artış sağladığı belirlenmiştir. Ayrıca, doz uygulaması yaprak klorofil miktarında bir artış sağlamıştır. Bu çalışmada, farklı dozlarda Tebuconazole uygulamalarının, hıyarda fide boyunu kontrol altına aldığı ve bazı fide kalite özelliklerine olumlu yönde etki ettiği belirlenmiştir.

Keywords

Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis sativus L.) Seedling via Morphological and Molecular Methods

Abstract

Aim of this study was to determine the effect of tebuconazole applications on cucumber seedling via morphological and molecular methods. In order to determine the most appropriate dose of different concentrations (25, 50, 75, 100, 125 and 150 ppm) of tebuconazole affecting the seedling quality and preventing possible genotoxic effects, retrotransposon motility at all doses was determined. Polymorphism rates and changes in GTS values were also determined. At the applied doses, polymorphism values were found as 0%, 5.55%, 16.66%, 38.88%, 55.55% and 61.11%, respectively. The GTS value was decreased from 100% to approximately 38.89% based on the applied doses. Statistical evaluations indicated that different dose applications resulted significant changes in the seedling characteristics. The seedling and stem heights were suppressed by 57.5% and 55.9%, respectively compared to the controls. The stem diameter increased 9.95% following the application. Overall, 50, 75 and 150 ppm doses were determined to achieve an increase in the dry matter and leaf chlorophyll content. In this study, applications of Tebuconazole at different doses controled the seedling height in cucumber and affected specific quality characteristics of the seedlings.

Keywords

Thanks

Thank you for the contributions of the Erzincan Horticultural Research Institute.

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Details

Primary Language

English

Subjects

Structural Biology, Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Hüseyin Bulut
0000-0003-3424-7012
Türkiye

Halil İbrahim Öztürk ^*
0000-0002-8977-0831
Türkiye

Atilla Dursun
0000-0002-8475-8534
Türkiye

Publication Date

October 31, 2021

Submission Date

June 18, 2020

Acceptance Date

January 25, 2021

Published in Issue

Year 2021 Volume: 24 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.754689

IZ

https://izlik.org/JA36AK44ES

Cite

RIS / Bibtex

APA

Bulut, H., Öztürk, H. İ., & Dursun, A. (2021). Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis sativus L.) Seedling via Morphological and Molecular Methods. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(5), 969-977. https://doi.org/10.18016/ksutarimdoga.vi.754689

AMA

1.Bulut H, Öztürk Hİ, Dursun A. Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis sativus L.) Seedling via Morphological and Molecular Methods. KSU J. Agric Nat. 2021;24(5):969-977. doi:10.18016/ksutarimdoga.vi.754689

Chicago

Bulut, Hüseyin, Halil İbrahim Öztürk, and Atilla Dursun. 2021. “Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis Sativus L.) Seedling via Morphological and Molecular Methods”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 24 (5): 969-77. https://doi.org/10.18016/ksutarimdoga.vi.754689.

EndNote

Bulut H, Öztürk Hİ, Dursun A (October 1, 2021) Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis sativus L.) Seedling via Morphological and Molecular Methods. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 24 5 969–977.

IEEE

[1]H. Bulut, H. İ. Öztürk, and A. Dursun, “Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis sativus L.) Seedling via Morphological and Molecular Methods”, KSU J. Agric Nat., vol. 24, no. 5, pp. 969–977, Oct. 2021, doi: 10.18016/ksutarimdoga.vi.754689.

ISNAD

Bulut, Hüseyin - Öztürk, Halil İbrahim - Dursun, Atilla. “Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis Sativus L.) Seedling via Morphological and Molecular Methods”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 24/5 (October 1, 2021): 969-977. https://doi.org/10.18016/ksutarimdoga.vi.754689.

JAMA

1.Bulut H, Öztürk Hİ, Dursun A. Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis sativus L.) Seedling via Morphological and Molecular Methods. KSU J. Agric Nat. 2021;24:969–977.

MLA

Bulut, Hüseyin, et al. “Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis Sativus L.) Seedling via Morphological and Molecular Methods”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 24, no. 5, Oct. 2021, pp. 969-77, doi:10.18016/ksutarimdoga.vi.754689.

Vancouver

1.Hüseyin Bulut, Halil İbrahim Öztürk, Atilla Dursun. Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis sativus L.) Seedling via Morphological and Molecular Methods. KSU J. Agric Nat. 2021 Oct. 1;24(5):969-77. doi:10.18016/ksutarimdoga.vi.754689

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