Determination of The Antimicrobial, Antioxidant and Cytotoxic Activity of Paulownia tomentosa Steud.

Şule İnci; Lütfiye Kadıoğlu Dalkılıç; Sevda Kırbag; Semih Dalkılıç

doi:10.18016/ksutarimdoga.vi.804177

TR EN

Paulownia tomentosa Steud.’un Antimikrobiyal, Antioksidan ve Sitotoksik Etkilerinin Belirlenmesi

Öz

Geleneksel Çin tıbbında kullanılan Pawlonia tomentosa sahip olduğu biyolojik aktiviteler sayesinde bronşit ve astım gibi hastalıkların tedavisinde kullanılmaktadır. Bunun yanı sıra antimikrobiyal, antioksidan, antikanser gibi etkilerinin olduğu ve dizanteri, bel soğukluğu, hemoroid gibi hastalıkların tedavisinde de kullanıldığı bilinmektedir. Bu çalışmada P. tomentosa’nın çiçek, petal, kabuk, yaprak ve odun kısımlarının metanol çözücüsünden elde edilen ekstraklarının antimikrobiyal aktivitesi disk difüzyon metoduna göre belirlenmiştir. Çiçek ve petal kısımlarının metanolden elde edilen ekstraktının farklı konsantrasyonlarının antioksidan aktivitesi 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürücü kapasitesi yöntemine göre tespit edilmiştir. Metanol, etanol ve hekzan gibi çözücülerden elde edilen ekstraktlarının farklı konsantrasyonlarının antikanser aktivitesi 3- (4,5-dimetiltiyazol-2-iil) -2,5-difenil tetrazolium bromür (MTT) test yöntemi kullanılarak belirlenmiştir. Sonuçta en iyi antimikrobiyal aktiviteyi P. tomentosa’nın çiçek kısmının metanol özütü S. aureus’a (18 mm) karşı gösterdiği tespit edilmiştir. P. tomentosa’nın antioksidan aktivitesinin artan konsantrasyonlara bağlı olarak arttığı belirlenmiştir. P. tomentosa’nın en iyi sitotoksik etkiyi hekzan ekstresinde gösterdiği tespit edilmiştir.

Anahtar Kelimeler

Determination of The Antimicrobial, Antioxidant and Cytotoxic Activity of Paulownia tomentosa Steud.

Abstract

Paulownia tomentosa, used in traditional Chinese medicine, is used in the treatment of diseases such as bronchitis and asthma thanks to its biological activities. In addition, it is known to have antimicrobial, antioxidant, anticancer effects and it is also used in the treatment of diseases such as dysentery, gonorrhea, hemorrhoids. In this study, the antimicrobial activity of extracts obtained from methanol solvent of flower and petal parts of P. tomentosa was determined according to the disk diffusion method. The antioxidant activity of the different concentrations of the extracts obtained from methanol solvent of flower and petal extracts was determined via the 2,2-diphenyl-1-picrilhydrazyl (DPPH) radical scavenging capacity method. Anticancer activity of different concentrations of extracts obtained from solvents such as methanol, ethanol and hexane was determined using the 3- (4,5-dimethylthiazole-2-yl) -2,5-diphenyl tetrazolium bromide (MTT) test method. As a result, it was found that methanol extract of the flower part of P. tomentosa showed the best antimicrobial activity against S. aureus (18 mm). It was determined that antioxidant activity of P. tomentosa increased depend to increasing concentrations. It was concluded that P. tomentosa has the best cytotoxic effect in hexane extract.

Keywords

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Şule İnci ^*
0000-0002-4022-5269
Türkiye

Lütfiye Kadıoğlu Dalkılıç
0000-0002-6791-3811
Türkiye

Sevda Kırbag
0000-0002-4337-8236
Türkiye

Semih Dalkılıç
0000-0002-6892-247X
Türkiye

Publication Date

August 31, 2021

Submission Date

October 2, 2020

Acceptance Date

December 3, 2020

Published in Issue

Year 2021 Volume: 24 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.804177

IZ

https://izlik.org/JA97BH65YT

Cite

RIS / Bibtex

APA

İnci, Ş., Kadıoğlu Dalkılıç, L., Kırbag, S., & Dalkılıç, S. (2021). Determination of The Antimicrobial, Antioxidant and Cytotoxic Activity of Paulownia tomentosa Steud. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(4), 701-706. https://doi.org/10.18016/ksutarimdoga.vi.804177

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